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Team:Freiburg/Notebook/12 July
From 2011.igem.org
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SophieCramer (Talk | contribs) (→Sequence Analysis) |
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===LOV3=== | ===LOV3=== | ||
S35: is K322999. This sequence is the LOVtap with the tryptophane promoter, RBS, mRFP and two terminators. | S35: is K322999. This sequence is the LOVtap with the tryptophane promoter, RBS, mRFP and two terminators. | ||
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| + | =Plastic binding site= | ||
| + | Picking colonies (M2a, M2b, M3), inoculating LB (Cm) and let grow overnight. Start: 17:15 | ||
Revision as of 15:15, 12 July 2011
Contents |
Sequence Analysis
Lysis cassette and heat induced promoter
Lysis cassette
S15b: (Lysis cassette K124014) is named K124017 in the registry Part without RBS at the beginning Part without antiholin the sequece is also mutated leading to wrong amino acids if combined with RBS there would be too many bases (8) between RBS and coding sequence part is likely to be not effective
Green light receptor
CcaR
S17: (CcaR with Prefix and RBS) is ok. Check RBS. S18: seems to be ok too.
pycA+terminator
S24: prefix and suffix not complete
cph8
S29: Sequence without part. Has to be done again.
LOV1
S33: LOVtap only
LOV3
S35: is K322999. This sequence is the LOVtap with the tryptophane promoter, RBS, mRFP and two terminators.
Plastic binding site
Picking colonies (M2a, M2b, M3), inoculating LB (Cm) and let grow overnight. Start: 17:15
