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Team:KAIST-Korea/Projects/report 4-Test
From 2011.igem.org
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<h1> Introduction </h1><br /> | <h1> Introduction </h1><br /> | ||
| - | <p | + | <p> The human eyes cannot perceive objects that are smaller than a certain size. Also, they cannot recognize light whose intensity is lower than an inherent threshold. We take these limitations into account to determine the number of fluorescent proteins that must accumulate before we can notice any fluorescence, and establish the minimum circular area required for us to perceive any fluorescence.</p> |
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| - | <h1 | + | <h1 style="border-top:1px solid white; padding-bottom:2px;"> Objective </h1><br /> |
| - | + | <p> Investigate concentration required for an amount of fluorescent protein in the E.coli that makes light from E.coli be visible for human.</p> | |
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| - | <h1 | + | <h1 style="border-top:1px solid white; padding-bottom:2px;"> Background </h1><br /> |
| - | + | <p> Human has the limits in vision. For our objective, we have to know about the limit of recognizing size of objects in human vision. This limit is called the ‘Minimum visible acuity’. The exact definition of minimum visible acuity is the minimum size of object that the human eyes can discern. In the table 1 Types of visual acuity(reference 1), the value of detection acuity(red box), ~1.0 arc second, is the minimum visible acuity that we take.</p> | |
<div style="border:2px solid gray; padding-right:7px; "> | <div style="border:2px solid gray; padding-right:7px; "> | ||
<img src="https://static.igem.org/mediawiki/2011/d/dc/Table_report4.jpg" width=667; /> | <img src="https://static.igem.org/mediawiki/2011/d/dc/Table_report4.jpg" width=667; /> | ||
<p> Table : Types of visual acuity <sup> 1 </sup></p> | <p> Table : Types of visual acuity <sup> 1 </sup></p> | ||
</div> | </div> | ||
| - | <p | + | <p> We assume that the E. coli is in a darkroom for discovering the minimum number of fluorescent protein. In this reason, we use 0.1 lx, the minimum intensity of light that cone cells in human can perceive. <sup>2</sup> In fig1 (a), There is a brief picture for minimum visible acuity. We choose that 1 second is the basic time scale, so have to know the number of fluorescent protein per 1 second. By our research, The range of the photon emitted time is wide, from |
<img src="https://static.igem.org/mediawiki/2011/3/35/%EC%88%98%EC%8B%9D1.png" width=45; height=20; /> | <img src="https://static.igem.org/mediawiki/2011/3/35/%EC%88%98%EC%8B%9D1.png" width=45; height=20; /> | ||
to <img src="https://static.igem.org/mediawiki/2011/b/b4/%EC%88%98%EC%8B%9D2.png" width=45; height=24; /> | to <img src="https://static.igem.org/mediawiki/2011/b/b4/%EC%88%98%EC%8B%9D2.png" width=45; height=24; /> | ||
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</p> | </p> | ||
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| - | <h1> Analysis & Result </h1><br /> | + | <h1 style="border-top:1px solid white; padding-bottom:2px;"> Analysis & Result </h1><br /> |
</div> | </div> | ||
Revision as of 16:43, 11 July 2011
Introduction
The human eyes cannot perceive objects that are smaller than a certain size. Also, they cannot recognize light whose intensity is lower than an inherent threshold. We take these limitations into account to determine the number of fluorescent proteins that must accumulate before we can notice any fluorescence, and establish the minimum circular area required for us to perceive any fluorescence.
Objective
Investigate concentration required for an amount of fluorescent protein in the E.coli that makes light from E.coli be visible for human.
Background
Human has the limits in vision. For our objective, we have to know about the limit of recognizing size of objects in human vision. This limit is called the ‘Minimum visible acuity’. The exact definition of minimum visible acuity is the minimum size of object that the human eyes can discern. In the table 1 Types of visual acuity(reference 1), the value of detection acuity(red box), ~1.0 arc second, is the minimum visible acuity that we take.
Table : Types of visual acuity 1
We assume that the E. coli is in a darkroom for discovering the minimum number of fluorescent protein. In this reason, we use 0.1 lx, the minimum intensity of light that cone cells in human can perceive. 2 In fig1 (a), There is a brief picture for minimum visible acuity. We choose that 1 second is the basic time scale, so have to know the number of fluorescent protein per 1 second. By our research, The range of the photon emitted time is wide, from
to 
Although it hasn’t any theoretical reason, we choose the photon emitted time by a fluorescent protein,
.3
We will proceed this modeling by using the energy of photons for relating the light and the number of fluorescent protein. One of the unit for light is lm(lumen) and this unit is transformed to J(joule) in
4
In fig1 (b), There are two values that we will use.
