"
Copenhagen/11 July 2011
From 2011.igem.org
(Difference between revisions)
(→Other Work) |
|||
| (7 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
==Monday== | ==Monday== | ||
| + | ===Lab Work=== | ||
| - | + | *2 overnight cultures of A2 was put in a shaking cabinet in 37 degrees | |
| + | |||
| + | *Biobrick A1 og A2 was [[Team:Copenhagen/Protocol#Mutations|mutated]] (again with template amounts 10,25 and 50 nm) and XL1-Blue competent cells were [[Team:Copenhagen/Protocol#Transformation|transformed]] | ||
| + | |||
| + | |||
| + | * We extended our cyp B1 with prefix and suffix by a [[Team:Copenhagen/Protocol#PCR Reaction|PCR approach]]. | ||
| + | |||
| + | The DNA obtained was purified by using a PCR purification kit, followed by gel purification and then [[Team:Copenhagen/Protocol#Gel Extraction Protocol|extracted]] the DNA from the gel. | ||
| + | The B1 DNA is now ready to be transfected into expression vectors. | ||
| + | |||
| + | ===Other Work=== | ||
| + | |||
| + | Our [[Team:Copenhagen/Safety|safety page]] has now been published!!! | ||
| + | |||
| + | [[Team:Copenhagen/Notebook|Back to Notebook]] | ||
Latest revision as of 15:03, 11 July 2011
Monday
Lab Work
- 2 overnight cultures of A2 was put in a shaking cabinet in 37 degrees
- Biobrick A1 og A2 was mutated (again with template amounts 10,25 and 50 nm) and XL1-Blue competent cells were transformed
- We extended our cyp B1 with prefix and suffix by a PCR approach.
The DNA obtained was purified by using a PCR purification kit, followed by gel purification and then extracted the DNA from the gel. The B1 DNA is now ready to be transfected into expression vectors.
Other Work
Our safety page has now been published!!!
