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Revision as of 09:05, 11 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→PCR for Ccas and CcaR) ← Older edit		Revision as of 09:09, 11 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→PCR for Ccas and CcaR) Newer edit →
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	1µl dNTPs<br/>		1µl dNTPs<br/>
	0,5 µl Phusion<br/>		0,5 µl Phusion<br/>
		+	<br/>
		+	<br/>
		+	==Miniprep==
		+
		+	'''Investigators: Julia, Sandra'''
		+
		+	<span style="color:blue;">Comments: Miniprep from june,14 was showed bad results. Grew over night at 37°C.</span>
		+
		+	Miniprep PR4a, PR4b, PR8a, PR8b, PR9a, PR9b<br/>
		+	measurement of nanodrop was ok<br/>

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Revision as of 09:09, 11 July 2011

??.Labday

PCR for Ccas and CcaR

Investigators: Julia

Ccas: cyanobacteriochrome is a green light receptor that induces the expression of pycobillisome linker protein
Ccar: cognate response regulator

Primer diluted, PCR for CcaS with P(rimer) 5, P7 and CcaR with P4, P6

32,5 µl H2O
2µl Primer fw
2µl Primer rev
1µl DNA ( synechocystis from Hess-Lab)
1µl dNTPs
0,5 µl Phusion

Miniprep

Investigators: Julia, Sandra

Comments: Miniprep from june,14 was showed bad results. Grew over night at 37°C.

Miniprep PR4a, PR4b, PR8a, PR8b, PR9a, PR9b
measurement of nanodrop was ok