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Team:Freiburg/Notebook/11 June
From 2011.igem.org
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<span style="color:blue;">Comments:For ligation digested samples were used from yesterday.</span> | <span style="color:blue;">Comments:For ligation digested samples were used from yesterday.</span> | ||
| - | Every promotor was ligated with every RBS | + | Every promotor was ligated with every RBS using the Ginko Bioworks 3A-assembly protocol |
PR1: S1 with S4 (660bp)<br/> | PR1: S1 with S4 (660bp)<br/> | ||
Revision as of 13:00, 8 July 2011
??.Labday
Ligation of promotor, RBS in vector
Investigators: Sophie, Theo, Sandra
Comments:For ligation digested samples were used from yesterday.
Every promotor was ligated with every RBS using the Ginko Bioworks 3A-assembly protocol
PR1: S1 with S4 (660bp)
PR2: S1 with S5 (1000bp)
PR3: S1 with S6 (426bp)
PR4: S2 with S4 (233bp)
PR5: S2 with S5 (940bp)
PR6: S2 with S6 (981bp)
PR7: S3 with S4
PR8: S3 with S5
PR9: S3 with S6
Name for each construct:
| sample | name | |
| Promotor | BBa_J23104 | S1 |
| Promotor | BBa_J23110 | S2 |
| Promotor | BBa_J23116 | S3 |
| RBS | BBa_B0034 | S4 |
| RBS | BBa_B0032 | S5 |
| RBS | BBa_B0031 | S6 |
| Vector | pSB1C3 | S7 |
