Team:Freiburg/Notebook/11 June

From 2011.igem.org

(Difference between revisions)
(Ligation of promotor, RBS in vector)
(Ligation of promotor, RBS in vector)
Line 19: Line 19:
PR8: S3 with S5<br/>
PR8: S3 with S5<br/>
PR9: S3 with S6<br/>
PR9: S3 with S6<br/>
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{| cellpadding="10" cellspacing="0" border="1"
 +
|
 +
|sample
 +
|name
 +
|-
 +
|Promotor
 +
|BBa_J23104
 +
|S1
 +
|-
 +
|Promotor
 +
|BBa_J23110
 +
|S2
 +
|-
 +
|Promotor
 +
|BBa_J23116
 +
|S3
 +
|-
 +
|RBS
 +
|BBa_B0034
 +
|S4
 +
|-
 +
|RBS
 +
|BBa_B0032
 +
|S5
 +
|-
 +
|RBS
 +
|BBa_B0031
 +
|S6
 +
|-
 +
|Vector
 +
|pSB1C3
 +
|S7
 +
|}

Revision as of 12:53, 8 July 2011

??.Labday

Ligation of promotor, RBS in vector

Investigators: Sophie, Theo, Sandra


Comments:For ligation digested samples were used from yesterday.

Every promotor was ligated with every RBS.

PR1: S1 with S4 (660bp)
PR2: S1 with S5 (1000bp)
PR3: S1 with S6 (426bp)
PR4: S2 with S4 (233bp)
PR5: S2 with S5 (940bp)
PR6: S2 with S6 (981bp)
PR7: S3 with S4
PR8: S3 with S5
PR9: S3 with S6

sample name
Promotor BBa_J23104 S1
Promotor BBa_J23110 S2
Promotor BBa_J23116 S3
RBS BBa_B0034 S4
RBS BBa_B0032 S5
RBS BBa_B0031 S6
Vector pSB1C3 S7