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Team:Nevada/Notebook/Temp
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<a href="https://2011.igem.org/Team:Nevada/Notebook/Temp"><font size=4>Weeks 1-4</font></a> | <a href="https://2011.igem.org/Team:Nevada/Notebook/Temp"><font size=4>Weeks 1-4</font></a> | ||
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<font size=6>Temp Calender</font> | <font size=6>Temp Calender</font> | ||
Revision as of 19:56, 7 July 2011
To edit on a specific week. Click on the edit button corresponding to the week.
Contents |
Week 1 - June 1st-5th
E. Coli
Comment Here
Cyano
Comment Here
Enzymology
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
* Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP
* 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH
Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations. Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme.
Media
Week 2 - June 6th-12th
E. Coli
Comment Here
Cyano
Comment Here
Enzymology
Comment Here
Media
Comment Here
Week 3 - June 13th-19th
E. Coli
Comment Here
Cyano
Comment Here
Enzymology
Comment Here
Media
Comment Here
Week 4 - June 20th-26th
E. Coli
Comment Here
Cyano
Comment Here
Enzymology
Comment Here
Media
