Team:Paris Liliane Bettencourt/Notebook/2011/07/05/

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(→ligation and transformation)
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<ul><li>BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
<ul><li>BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
</li><li>BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL
</li><li>BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL
 +
</li></ul>
 +
 +
<p style="display:block;"><font color="red">Babak</font>
 +
</p><p style="display:block;"><u>Gel purification</u>
 +
</p>
 +
<ul><li>well 1: ladder 100pb
 +
</li><li>from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL
 +
</li></ul>
 +
 +
<p style="display:block;"><u>Digestion</u><ul><li>BBa_K090403 cut by EcoR1 and XbaI
 +
</li><li>BBa_K143079 cut by EcoR1 and SpeI
 +
</li><li>BBa_B0015 cut by EcoR1 and XbaI
 +
</li><li>BBa_E1010 cut by EcoR1 and SpeI
 +
</li><li>B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI
</li></ul>
</li></ul>

Revision as of 13:51, 7 July 2011

ligation and transformation

ligation

  • BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
  • BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL

Babak

Gel purification

  • well 1: ladder 100pb
  • from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL

Digestion

  • BBa_K090403 cut by EcoR1 and XbaI
  • BBa_K143079 cut by EcoR1 and SpeI
  • BBa_B0015 cut by EcoR1 and XbaI
  • BBa_E1010 cut by EcoR1 and SpeI
  • B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI

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