Copenhagen/7 July 2011

(Difference between revisions)

Latest revision as of 12:34, 7 July 2011

The transformation of Cyp79B1 went well, we have a lot of colonies. The sequentation of A1 showed that it was not mutated.

Mutation and transformation of A1 and A2 with an other amount of template (from 10/50 ng to 25 ng)

We have answered many e-mails from danish companies associated with the biotech industry. Some supported us morally and a few economically. We a looking forward to recieve positive answers from the rest of the companies.

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-==Wednesday==
+==Thursday==
+The transformation of Cyp79B1 went well, we have a lot of colonies.
+The sequentation of A1 showed that it was not mutated.
-We continued our quest to make mutant cyp's. We still need CYP79A2 and CYP79B1 (maybe CYP79A1 but we certaintly hope not)
-We still haven't gotten our sequence from the CYP79A1 we purified yesterday so we are not completely sure what it is, but we believe in positive thinking and have added prefix and suffix to it, in order to make our first BioBrick.
 ===Lab Work===
-* [[Team:Copenhagen/Protocol#Mutations|Digested]] our template plasmid
+* PCR purification of A1
+* [[Team:Copenhagen/Protocol#Mutations|Mutation]] and [[Team:Copenhagen/Protocol#Transformations|transformation]] of A1 and A2 with an other amount of template (from 10/50 ng to 25 ng)
+* Overnight culture of B1 (2 of each template concentration).
-* [[Team:Copenhagen/Protocol#Transformations|Transformed]] XL1-Blue with mutants CYP79A1, CYP79A2 and CYP79B1
+===Other Work===
-* Did a [[Team:Copenhagen/Protocol#PCR_Reaction|PCR]] with mutant CYP79A1 and primers designed with prefix and suffix
+*We have answered many e-mails from danish companies associated with the biotech industry. Some supported us morally and a few economically. We a looking forward to recieve positive answers from the rest of the companies.
-Back to [[Team:Copenhagen/Notebook|Notebook]]
+Back to [[Team:Copenhagen/Notebook|notebook]]