Team:Lethbridge/Notebook

From 2011.igem.org

(Difference between revisions)
(→April 9th - April 30th, 2011)
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====Group 1 (JV, DG, AV, IW)====
====Group 1 (JV, DG, AV, IW)====
#<u>Assembly Round 1</u>
#<u>Assembly Round 1</u>
-
##K331033 + K331035
+
##K331033 + K331035  
-
##R0040 + K331023
+
##R0040 + K331023 ==> K331031 (Already Made)
-
##K331025 + B0015
+
##K331025 + B0015  
-
##R0040 + K331039
+
##R0040 + K331039  
-
##K331039 + B0015
+
##K331039 + B0015  
#<u>Assembly Round 2</u>
#<u>Assembly Round 2</u>
## 1.2 + 1.3
## 1.2 + 1.3
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====Group 2 (HB, BL, MC, BV)====
====Group 2 (HB, BL, MC, BV)====
#<u>Assembly Round 1</u>
#<u>Assembly Round 1</u>
-
##R0010 + B0034
+
##R0010 + B0034  
-
##K249002 + B0015
+
##K249002 + B0015  
##I13452 + B0034
##I13452 + B0034
##C0040 + B0015
##C0040 + B0015

Revision as of 22:48, 9 April 2011




Contents

Assignments

April 9th - April 30th, 2011

Group 1 (JV, DG, AV, IW)

  1. Assembly Round 1
    1. K331033 + K331035
    2. R0040 + K331023 ==> K331031 (Already Made)
    3. K331025 + B0015
    4. R0040 + K331039
    5. K331039 + B0015
  2. Assembly Round 2
    1. 1.2 + 1.3
    2. 1.4 + B0015
    3. R0040 + 1.4

Characterize Fluorescence of Parts 1.1, 2.1, 2.2, 2.3

Group 2 (HB, BL, MC, BV)

  1. Assembly Round 1
    1. R0010 + B0034
    2. K249002 + B0015
    3. I13452 + B0034
    4. C0040 + B0015
  2. Assembly Round 2
    1. 1.1 + 1.2
    2. 1.3 + 1.4
  3. Assembly Round 3
    1. 2.1 + 2.2

Group 3 (JS, RP, KG, SD)

  • Use PCR to alter suffixes to include fusion standards for xylE and mms6
  • Assemble fusion-ready xylE and mms6 with S04261 (C-terminal Arginine tail + B0015)
  • Move these BioBricks from synthesis vectors (either pMA-T or pMA-RQ) into pSB1C3
    • K331007
    • K331008
    • K331009
    • K331012
    • K331022
    • K331024
    • K331025

Group Member Pages

JV & DG

AV & IW

JS & RP

HB & BL

MC & BV

KG & SD

Protocols

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