Copenhagen/4 July 2011

From 2011.igem.org

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===Lab Work===
===Lab Work===
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* On the A1 plate from friday, there were one colony. Big succes. We made an overnightculture.
* We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out.
* We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out.

Revision as of 11:13, 5 July 2011

Monday

The rest of the team have returned, happy, well rested and eager to begin working.

Lab Work

  • On the A1 plate from friday, there were one colony. Big succes. We made an overnightculture.
  • We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out.
  • We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program.

Afterwards we digested our mutants with Dnp - see protocols Mutations


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