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Team:Paris Liliane Bettencourt/Notebook/2011/07/04/
From 2011.igem.org
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<ul><li>well 1: ladder 1kb | <ul><li>well 1: ladder 1kb | ||
</li><li>well 2: BBa_B0015 cut by EcoR1 and XbaI | </li><li>well 2: BBa_B0015 cut by EcoR1 and XbaI | ||
| - | </li><li>well 3: BBa_E1010 cut by EcoR1 and SpeI | + | </li><li>well 3: BBa_E1010 cut by EcoR1 and SpeI didn't work |
</li><li>well 4: BBa_K145001 cut by EcoR1 and SpeI | </li><li>well 4: BBa_K145001 cut by EcoR1 and SpeI | ||
</li><li>well 5: BBa_K090403 cut by EcoR1 and XbaI | </li><li>well 5: BBa_K090403 cut by EcoR1 and XbaI | ||
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</p> | </p> | ||
<ul><li>well 1: ladder 1kb | <ul><li>well 1: ladder 1kb | ||
| - | </li><li>from well 2: BBa_B0015 cut by EcoR1 and XbaI, concentration of DNA= | + | </li><li>from well 2: BBa_B0015 cut by EcoR1 and XbaI, concentration of DNA=10.9ng/µL |
| - | + | </li><li>from well 4: BBa_K145001 cut by EcoR1 and SpeI, concentration of DNA=8.5ng/µL | |
| - | </li><li>from well 4: BBa_K145001 cut by EcoR1 and SpeI, concentration of DNA= | + | </li><li>from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL |
| - | </li><li>from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA= | + | </li><li>from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL |
| - | </li><li>from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA= | + | |
</li></ul> | </li></ul> | ||
Revision as of 10:24, 5 July 2011
Minipreps, digestion, electrophoresis and purification
Axel
Minipreps
- on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
- on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
- on BBa_B0015, concentration of DNA=408.1ng/µL
- on BBa_E1010 concentration of DNA=330.8ng/µL
- on BBa_K145001, concentration of DNA=572.8ng/µL
digestion
- BBa_K090403 cut by EcoR1 and XbaI
- BBa_K143079 cut by EcoR1 and SpeI
- BBa_B0015 cut by EcoR1 and XbaI
- BBa_E1010 cut by EcoR1 and SpeI
- BBa_K145001 cut by EcoR1 and SpeI
gel electrophoresis at 0.6% of Agarose
- well 1: ladder 1kb
- well 2: BBa_B0015 cut by EcoR1 and XbaI
- well 3: BBa_E1010 cut by EcoR1 and SpeI didn't work
- well 4: BBa_K145001 cut by EcoR1 and SpeI
- well 5: BBa_K090403 cut by EcoR1 and XbaI
- well 6: BBa_K143033 cut by EcoR1 and SpeI
gel purification
- well 1: ladder 1kb
- from well 2: BBa_B0015 cut by EcoR1 and XbaI, concentration of DNA=10.9ng/µL
- from well 4: BBa_K145001 cut by EcoR1 and SpeI, concentration of DNA=8.5ng/µL
- from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL
- from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL
