Team:Paris Liliane Bettencourt/Notebook/2011/07/04/

From 2011.igem.org

(Difference between revisions)
(→Minipreps, digestion, purification and ligation)
(→Minipreps, digestion, purification and ligation)
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<br />10 µL of minipreped DNA corresponds to approximately 125ng of DNA (see fluorescence of the 3Kb band of the ladder)
 
</p><p style="display:block;"><u>digestion</u>
</p><p style="display:block;"><u>digestion</u>
-
 
<ul><li>BBa_K090403 cut by EcoR1 and XbaI
<ul><li>BBa_K090403 cut by EcoR1 and XbaI
</li><li>BBa_K143079 cut by EcoR1 and SpeI
</li><li>BBa_K143079 cut by EcoR1 and SpeI
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[[File:electrophoresis 040711.jpg|thumb]]
[[File:electrophoresis 040711.jpg|thumb]]
</p><p style="display:block;"><u>gel electrophoresis at 0.6% of Agarose</u>
</p><p style="display:block;"><u>gel electrophoresis at 0.6% of Agarose</u>
 +
</p>
 +
<ul><li>well 1: ladder 1kb
 +
</li><li>well 2: BBa_B0015 cut by EcoR1 and XbaI
 +
</li><li>well 3: BBa_E1010 cut by EcoR1 and SpeI
 +
</li><li>well 4: BBa_K145001 cut by EcoR1 and SpeI
 +
</li><li>well 5: BBa_K090403 cut by EcoR1 and XbaI
 +
</li><li>well 6: BBa_K143033 cut by EcoR1 and SpeI
 +
</li></ul>
 +
 +
</p><p style="display:block;"><u>gel purification</u>
</p>
</p>
<ul><li>well 1: ladder 1kb
<ul><li>well 1: ladder 1kb

Revision as of 09:48, 5 July 2011

Minipreps, digestion, purification and ligation

Axel

Minipreps

  • on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
  • on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
  • on BBa_B0015, concentration of DNA=408.1ng/µL
  • on BBa_E1010 concentration of DNA=330.8ng/µL
  • on BBa_K145001, concentration of DNA=572.8ng/µL


</p>

digestion

  • BBa_K090403 cut by EcoR1 and XbaI
  • BBa_K143079 cut by EcoR1 and SpeI
  • BBa_B0015 cut by EcoR1 and XbaI
  • BBa_E1010 cut by EcoR1 and SpeI
  • BBa_K145001 cut by EcoR1 and SpeI
Electrophoresis 040711.jpg

gel electrophoresis at 0.6% of Agarose

  • well 1: ladder 1kb
  • well 2: BBa_B0015 cut by EcoR1 and XbaI
  • well 3: BBa_E1010 cut by EcoR1 and SpeI
  • well 4: BBa_K145001 cut by EcoR1 and SpeI
  • well 5: BBa_K090403 cut by EcoR1 and XbaI
  • well 6: BBa_K143033 cut by EcoR1 and SpeI
</p>

gel purification

  • well 1: ladder 1kb
  • well 2: BBa_B0015 cut by EcoR1 and XbaI
  • well 3: BBa_E1010 cut by EcoR1 and SpeI
  • well 4: BBa_K145001 cut by EcoR1 and SpeI
  • well 5: BBa_K090403 cut by EcoR1 and XbaI
  • well 6: BBa_K143033 cut by EcoR1 and SpeI
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