"

From 2011.igem.org

Revision as of 09:45, 5 July 2011 (view source) AxelS (Talk | contribs) (→Minipreps, digestion, purification and ligation) ← Older edit		Revision as of 09:45, 5 July 2011 (view source) AxelS (Talk | contribs) (→Minipreps, digestion, purification and ligation) Newer edit →
Line 33:		Line 33:
	</li></ul>		</li></ul>
-	<p style="display:block;"><a href="/wiki/Image:electrophoresis 040711.jpg" class="image" title="Image:electrophoresis 040711.jpg"><img alt="Image:electrophoresis 040711.jpg" src="/images/9/99/electrophoresis 040711.jpg" width="425" height="539" border="0" /></a>	+	[[File:electrophoresis 040711.jpg]]

---

Revision as of 09:45, 5 July 2011

Minipreps, digestion, purification and ligation

Axel

Minipreps

• on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
• on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
• on BBa_B0015, concentration of DNA=408.1ng/µL
• on BBa_E1010 concentration of DNA=330.8ng/µL
• on BBa_K145001, concentration of DNA=572.8ng/µL

10 µL of minipreped DNA corresponds to approximately 125ng of DNA (see fluorescence of the 3Kb band of the ladder)

</p>

digestion

• BBa_K090403 cut by EcoR1 and XbaI
• BBa_K143079 cut by EcoR1 and SpeI
• BBa_B0015 cut by EcoR1 and XbaI
• BBa_E1010 cut by EcoR1 and SpeI
• BBa_K145001 cut by EcoR1 and SpeI

gel electrophoresis at 0.6% of Agarose

• well 1: ladder 1kb
• well 2: BBa_B0015 cut by EcoR1 and XbaI
• well 3: BBa_E1010 cut by EcoR1 and SpeI
• well 4: BBa_K145001 cut by EcoR1 and SpeI
• well 5: BBa_K090403 cut by EcoR1 and XbaI
• well 6: BBa_K143033 cut by EcoR1 and SpeI