Team:Grenoble/Projet/Design

From 2011.igem.org

(Difference between revisions)
 
(96 intermediate revisions not shown)
Line 7: Line 7:
<html>
<html>
-
<div class="body">
 
-
 
-
<div class="left">
 
-
<h1>Design and principle</h1>
 
-
<div class="blocbackground">
 
-
<h2><a href="#toggle">Toggle Switch</a></h2>
 
-
<h2><a href="#qs">Quorum Sensing</a></h2>
 
-
</div>
 
-
 
<!--
<!--
-
contenu de la page
+
<img src="http://www.clickartists.org/clicksite/html/images/square.jpg"/>
 +
 
 +
Si vous éditez la page commencez par décommenter ces lignes, publier, PUIS commencer à faire ce que vous avez à faire et quand vous avez fini de publier remettez en commentaire.
 +
 
 +
Ne laissez pas le carré trop longtemps si vous n'éditez pas, chaque fois reprenez ce qui est sur internet plutôt que ce que vous avez sur votre PC
 +
ps: dans Geany selectionner une ou plusieurs lignes et appuyer sur "Ctrl + E" pour commenter ou décommenter
-->
-->
-
<h2 id="toggle">Toggle switch explanation</h2>
+
<div class="body">
-
+
 
-
<h3>In a few words</h3>
+
<div class="left">
 +
 +
<div  class="blocbackground">
-
<div class="blocbackground">
+
<h1>Genetic Network</h1>
<p>
<p>
-
Our project is based on a toggle switch system. So we have to explain how it works. Basically it corresponds to an irreversible choice between two pathways depending on the environment. In reality, the reversibility is possible but very difficult to obtain because of the strength of the interactions and the stability of the system (defined by hysteresis * insérer un lien ici*).  
+
we design the following circuit, based on Gardner and al. Gardner's <a href="#1">[1]</a> work and balagadd and al. on Predator–Prey ecosystem <a href="#2">[2]</a>.
-
<br/>
+
-
<a href="https://static.igem.org/mediawiki/2011/a/a8/Event_tree.png"><img src="https://static.igem.org/mediawiki/2011/a/a8/Event_tree.png" alt="Event tree, bacteria out of the lab" title="Event Tree, bacteria are out of the lab ! What would happen ?"></a>
+
-
<!--
+
-
width="610"
+
-
></a>
+
-
-->
+
-
<br/>
+
</p>
</p>
-
</div>
+
-
+
<center><a href="https://static.igem.org/mediawiki/2011/8/80/Reseau_regulation_compl2_%281%29.png"><img height="400" src="https://static.igem.org/mediawiki/2011/8/80/Reseau_regulation_compl2_%281%29.png"></a></center>
-
<h3>Genetically, what is happening ?</h3>
+
<br/>
-
+
<br/>
-
<div class="blocbackground">
+
<p>
<p>
-
Contrary to us, bacteria can’t think or make voluntary choices. So how can they choose a pathway? Through our project, we are creating a genetic system which helps bacteria to take such a decision. For that, we exploit chemical components of the environment which influence the bacterial behaviour. By taking into account two proteins repressing each other’s expression (LacI and XR), we create the fundaments of a toggle system that can switch when using two inducers (IPTG and X) in the medium. 
+
It can be divided into four parts: the Toggle Switch, the Quorum Sensing, the Coloration and the post-transcriptional regulation. (Coloration is included in Quorum Sensing page) </p>
-
<a href="https://static.igem.org/mediawiki/2011/a/a8/Event_tree.png"><img src="https://static.igem.org/mediawiki/2011/a/a8/Event_tree.png" alt="Event tree, bacteria out of the lab" title="Event Tree, bacteria are out of the lab ! What would happen ?"></a>
+
<br/>
-
</p>
+
<br/>
</div>
</div>
-
<div class="blocbackground">
+
        <div class="blocbackground">
-
<table>
+
<h2>Table of content</h2>
-
<tr>
+
-
  <td></td>
+
-
  <td></td>
+
-
</tr>
+
-
<tr>
+
-
  <td></td>
+
-
  <td></td>
+
-
</tr> 
+
-
</table>
+
-
</div>
+
 +
<p>
 +
In the following pages, you can find the explanation about the differents sub-parts of our genetic network. Moreover we explane to you the importance of both to our project.
 +
</p>
-
<h2 id="qs">Quorum Sensing</h2>
+
For an introduction to the genetic circuit, see our tutorials in the human practice
-
+
section or download them here :
-
<div class="blocbackground">
+
<ul>
-
<p>
+
<li>
-
<dl>
+
<a href="https://static.igem.org/mediawiki/2011/9/91/M_for_B.png">
-
<dt>Ultra violet lamp: </dt>
+
Modelling for biologist</a>
-
<dd>
+
</li>
-
There is a risk for the eyes and the skin, but the UV lamp is only used to take a picture of our gel after electrophoresis, so we are never directly exposed because there is a protective cover and we wear a mask that shields from UV.
+
<li>
-
</dd>
+
<a href="https://static.igem.org/mediawiki/2011/5/54/B_for_M.png">
-
<dt>Centrifuge:</dt>
+
Synthetic Biology for modellers</a>
-
<dd>
+
</li>
-
The centrifuges have to be perfectly balanced. All the centrifuges in our lab have detectors that warn the operator in case of imbalance.  
+
</ul>
-
</dd>
+
 
-
<dt>Autoclave:</dt>
+
<br/><br/>
-
<dd>
+
 
-
The operation of the autoclave require a specific training and has only be performed by trained people.
+
<a href="https://2011.igem.org/Team:Grenoble/Projet/Design/toggle"><img class="icon" src="https://static.igem.org/mediawiki/2011/2/20/Bouton_toggle.png"/></a>
-
</dd>
+
-
</dl>
+
<big><big><a href="https://2011.igem.org/Team:Grenoble/Projet/Design/toggle" class="menu">Toggle Switch:</a></big></big><br/>
 +
<br/>
 +
<br/>
 +
<br/>
 +
<br/>
 +
<a href="https://2011.igem.org/Team:Grenoble/Projet/Design/quorum" class="menu"><img class="icon" src="https://static.igem.org/mediawiki/2011/f/f8/Bouton_QS_color.png"/></a>
 +
 +
<big><big><a href="https://2011.igem.org/Team:Grenoble/Projet/Design/quorum" class="menu">Quorum Sensing :</a></big></big><br/>
 +
 
 +
 +
<a  href="https://2011.igem.org/Team:Grenoble/Projet/Design/quorum" class="menu">The Quorum Sensing</a><br/>
 +
<a  href="https://2011.igem.org/Team:Grenoble/Projet/Design/quorum#coloration" class="menu">Coloration</a><br/>
 +
 
 +
<br/>
 +
<br/>
 +
<a href="https://2011.igem.org/Team:Grenoble/Projet/regulation" ><img class="icon" src="https://static.igem.org/mediawiki/2011/9/97/Bouton_regulation.png"/></a>
 +
 +
<big><big><a href="https://2011.igem.org/Team:Grenoble/Projet/regulation" class="menu">Post-transcriptional regulation :</a></big></big><br/>
 +
 
 +
<a href="https://2011.igem.org/Team:Grenoble/Projet/regulation" class="menu">A post-transcriptional regulation system</a><br/>
 +
<a  href="https://2011.igem.org/Team:Grenoble/Projet/regulation#rsma" class="menu">The RsmA translational regulation system</a><br/>
 +
 
 +
<a  href="https://2011.igem.org/Team:Grenoble/Projet/regulation#rpos" class="menu">The rpoS regulation system</a><br/>
 +
<br/><br/><br/>
 +
</p>
</p>
 +
<p>
 +
You can find the results of our biological experiments in the
 +
<a href="https://2011.igem.org/Team:Grenoble/Projet/Results">results page
 +
</a>.
 +
        </p>
</div>
</div>
-
 
+
 +
<!-- <div class="blocbackground">
 +
 +
<h2>References :</h2>
 +
 +
<ol>
 +
<li id="1">
 +
GARDNER'S WORK: "Construction of a genetic toggle switch in Escherichia coli", Timothy S. Gardner, Charles R. Cantor and James J. Collins, Nature, 2000, Vol.403, p339-42.
 +
</li>
 +
<li id="2">
 +
PREY AND PREDATOR: "A synthetic Escherichia coli predator–prey ecosystem", Frederick K Balagadd, Hao Song, Jun Ozaki, Cynthia H Collins, Matthew Barnet, Frances H Arnold, Stephen R Quake and Lingchong You, Molecular Systems Biology, 2008;4:187.
 +
</li>
 +
</ol>
 +
 +
</div> -->
 +
</div>
</div>
</div>
</div>
 +
<center>
 +
<form method="get" >
 +
  <input type="button" value="< PREVIOUS <" onclick="document.location = 'https://2011.igem.org/Team:Grenoble/Projet/Design';" />
 +
  <select name="id" onchange="document.location = '/Team:Grenoble/Projet' + this.options[this.selectedIndex].value ;">
 +
 +
    <optgroup label="Genetic network Homepage">
 +
   
 +
    <option value="#Content" selected="selected">General network</option>
 +
 +
    </optgroup>
 +
 +
 +
    <optgroup label="Toggle Switch" >
 +
                               
 +
                                    <option value="/Design/toggle" >The Toggle Switch</option>
 +
 +
                            </optgroup>
 +
                       
 +
                       
 +
                            <optgroup label="Quorum Sensing">
 +
                               
 +
                                    <option value="/Design/quorum">The Quorum Sensing</option>
 +
                               
 +
                                    <option value="/Design/quorum#coloration">The Coloration</option>
 +
                               
 +
 +
                            </optgroup>
 +
 +
                            <optgroup label="Post Transcriptional Regulation">
 +
                           
 +
    <option value="/regulation">A Post-Transcriptional Regulation System</option>
 +
    <option value="/regulation#rsma">The RsmA Translational Regulation System</option>
 +
    <option value="/regulation#rpos">The rpoS regulation system</option>
 +
 +
    </optgroup>
 +
   
 +
   
 +
                    </select>
 +
                    <input type="hidden" name="id2" value="0" />
 +
                    <input type="submit" value="Go!" />
 +
  <input type="button" value="> NEXT >" onclick="document.location = '/Team:Grenoble/Projet/Design/toggle';" />
 +
               
 +
            </form></center>
 +
</html>
</html>
 +
 +
{{:Team:Grenoble/Design/pied}}

Latest revision as of 18:24, 31 January 2012

Grenoble 2011, Mercuro-Coli iGEM


Genetic Network

we design the following circuit, based on Gardner and al. Gardner's [1] work and balagadd and al. on Predator–Prey ecosystem [2].



It can be divided into four parts: the Toggle Switch, the Quorum Sensing, the Coloration and the post-transcriptional regulation. (Coloration is included in Quorum Sensing page)



Table of content

In the following pages, you can find the explanation about the differents sub-parts of our genetic network. Moreover we explane to you the importance of both to our project.

For an introduction to the genetic circuit, see our tutorials in the human practice section or download them here :

Toggle Switch:




Quorum Sensing :
The Quorum Sensing
Coloration


Post-transcriptional regulation :
A post-transcriptional regulation system
The RsmA translational regulation system
The rpoS regulation system



You can find the results of our biological experiments in the results page .

logo iGEM
iGEM 2011 Main Page


Contact Us:

Clic here !

Retrieved from "http://2011.igem.org/Team:Grenoble/Projet/Design"