"
Copenhagen/4 July 2011
From 2011.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
* We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out. | * We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out. | ||
| - | * We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program. | + | * We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program. |
| + | Afterwards we digested our mutants with Dnp [[Team:Copenhagen/Protocol#Mutations]] | ||
| - | * | + | * Transformed ''XL1-Blue'' competent cells - protocols [[Team:Copenhagen/Protocol##Transformations]] |
Back to [[Team:Copenhagen/Notebook|Notebook]] | Back to [[Team:Copenhagen/Notebook|Notebook]] | ||
Revision as of 12:18, 4 July 2011
Monday
The rest of the team have returned, happy, well rested and eager to begin working.
Lab Work
- We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out.
- We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program.
Afterwards we digested our mutants with Dnp Team:Copenhagen/Protocol#Mutations
- Transformed XL1-Blue competent cells - protocols Team:Copenhagen/Protocol##Transformations
Back to Notebook
