"
Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme
From 2011.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 2: | Line 2: | ||
{{Lyon-INSA-ENS/blocStyle}} | {{Lyon-INSA-ENS/blocStyle}} | ||
{{INSA-Lyon/styletestaurelie}} | {{INSA-Lyon/styletestaurelie}} | ||
| - | {{Lyon-INSA-ENS/ | + | {{Lyon-INSA-ENS/menuhorizontal}} |
| - | {{Lyon-INSA-ENS/menuNotebookProtocoles}} | + | <!--{{Lyon-INSA-ENS/menuNotebookProtocoles}}--> |
| - | + | {{Lyon-INSA-ENS/menuNotebookVertical|Protocols = actif}} | |
<html> | <html> | ||
Latest revision as of 10:20, 10 January 2012
Ozyme digestion
This protocol aims at cutting plasmids on specific restriction sites using restriction enzymes.
Procedure
1. In an eppendorf tube, add the following solutions in any order :
-11µL water
-10µL DNA
-2.5µL NE Buffer 2
-0.5µL BSA X50
2. Add 0.5µL of each one of the desired enzymes.
3. Incubate at 37°C for 10mn
4. Inactivate the enzymes by a 20mn incubation at 80°C
