"
Team:MIT
From 2011.igem.org
| Line 11: | Line 11: | ||
<h3>Tissues by Design</h3> | <h3>Tissues by Design</h3> | ||
| - | <p>Our project focuses on genetically programming tissue self-construction to achieve specific patterns of cell differentiation (initially with fluorescence, ultimately with cell fate regulators). | + | <p>Our project focuses on genetically programming tissue self-construction to achieve specific patterns of cell differentiation (initially with fluorescence, ultimately with cell fate regulators). To accomplish we focused on three components: cell-cell communication pathways, intracellular information processing circuits, and cell-cell adhesion. Through engineered control of these mechanisms, we are investigating how programmed local rules of interactions between cells can lead to the emergence of desired global patternings.</p> |
<br> | <br> | ||
<p><img src="https://static.igem.org/mediawiki/2011/5/51/Simulation.jpg" style="max-width:800px; margin-right:10px;"/></p></br> | <p><img src="https://static.igem.org/mediawiki/2011/5/51/Simulation.jpg" style="max-width:800px; margin-right:10px;"/></p></br> | ||
| - | + | <p>This is the result of a simulation run, starting with undifferentiated cells and ending with a pattern.</p> | |
<br><p>Specifically, we've developed a modular juxtacrine cell-cell signaling platform, using Notch and Delta proteins. | <br><p>Specifically, we've developed a modular juxtacrine cell-cell signaling platform, using Notch and Delta proteins. | ||
Revision as of 03:49, 29 October 2011
Tissues by Design
Our project focuses on genetically programming tissue self-construction to achieve specific patterns of cell differentiation (initially with fluorescence, ultimately with cell fate regulators). To accomplish we focused on three components: cell-cell communication pathways, intracellular information processing circuits, and cell-cell adhesion. Through engineered control of these mechanisms, we are investigating how programmed local rules of interactions between cells can lead to the emergence of desired global patternings.
This is the result of a simulation run, starting with undifferentiated cells and ending with a pattern.
Specifically, we've developed a modular juxtacrine cell-cell signaling platform, using Notch and Delta proteins. Below(left) is a simulation of pattern formation of cells with different genetic circuits. Below(right) is an animation depicting showing the Notch-Delta interaction leading to the cleavage of the Notch intracellular domain, which enters the nucleus and leads to the expression of cadherins, which cause cells to adhere to similarly expressing cells.
