"
Team:UTP-Panama/Results
From 2011.igem.org
(→Spectrophotometry Results) |
(→Discussion of Results) |
||
| (14 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
[[Team:UTP-Panama/Experiments2| '''Experiment 2 ''']] | | [[Team:UTP-Panama/Experiments2| '''Experiment 2 ''']] | | ||
[[Team:UTP-Panama/Experiments3| '''Experiments 3 ''']] | | [[Team:UTP-Panama/Experiments3| '''Experiments 3 ''']] | | ||
| + | [[Team:UTP-Panama/Experiments4| '''Experiments 4 ''']] | | ||
| + | [[Team:UTP-Panama/Experiments5| '''New Results''']] | | ||
[[Team:UTP-Panama/Results|'''Results''']]| | [[Team:UTP-Panama/Results|'''Results''']]| | ||
| Line 10: | Line 12: | ||
<br> | <br> | ||
| - | '''Experiments with BBa_K381001''' | + | <div align="center">'''Experiments with BBa_K381001''' |
| - | [[File:unonuevo.jpg]]<br> | + | [[File:unonuevo.jpg|center|490px|]]<br> |
| - | From these data we could conclude that: | + | |
| + | <div align="justify">From these data we could conclude that: | ||
• The BB grows best in LB. | • The BB grows best in LB. | ||
| Line 28: | Line 31: | ||
| - | '''Experiments with BBa_K410000''' | + | <div align="center">'''Experiments with BBa_K410000''' |
| - | [[File:dosnuevo.jpg]]<br> | + | [[File:dosnuevo.jpg|center|490px|]]<br> |
| - | From these data we could conclude that: | + | |
| + | <div align="justify">From these data we could conclude that: | ||
• The BB grows best in LB. | • The BB grows best in LB. | ||
| Line 46: | Line 50: | ||
| - | '''Experiments with BBa_K672000''' | + | <div align="center">'''Experiments with BBa_K672000'''<div align="center"> |
| - | [[File:tresnuevo.jpg]]<br> | + | [[File:tresnuevo.jpg|center|490px|]]<br> |
| - | From these data we could conclude that: | + | |
| + | <div align="justify">From these data we could conclude that: | ||
• The BB grows best in LB. | • The BB grows best in LB. | ||
| Line 62: | Line 67: | ||
• It shows almost no growth @8°C | • It shows almost no growth @8°C | ||
| + | |||
| + | |||
| + | We also studied the behavior of BBa_K381001 @23°C and @37°C in LB and Minimal Media; we detected the GFP expression using the spectophotometer, the graphs are shown below. | ||
| + | |||
| + | [[File:bristolnuevo.jpg|center|490px|]]<br> | ||
== Graphs: == | == Graphs: == | ||
| + | |||
| + | <div align="center">'''Experiments 1, 2 & 3:'''</div> | ||
| + | |||
[[Image:8grados.jpg|center|700px]] | [[Image:8grados.jpg|center|700px]] | ||
| Line 74: | Line 87: | ||
[[Image:37grados.jpg|center|700px]] | [[Image:37grados.jpg|center|700px]] | ||
| + | |||
| + | |||
| + | <div align="center">'''Other Experiments with Bristol:'''</div> | ||
| + | |||
| + | |||
| + | [[Image:023grados.jpg|center|600px]] | ||
| + | |||
| + | |||
| + | [[Image:037grados.jpg|center|600px]] | ||
==Discussion of Results== | ==Discussion of Results== | ||
| Line 80: | Line 102: | ||
Looking at the results for BBa_410000, it decreases the capability of BBa_381001, under a cold shock. | Looking at the results for BBa_410000, it decreases the capability of BBa_381001, under a cold shock. | ||
| + | |||
| + | From these results we could determine sources of errors during the experiments and learn from these for our further investigations. | ||
''The next step in our project:''<br> | ''The next step in our project:''<br> | ||
Latest revision as of 03:42, 29 October 2011
 Home |
Experiment 1 | Experiment 2 | Experiments 3 | Experiments 4 | New Results | Results| Spectrophotometry Results
Experiments with BBa_K381001
 From these data we could conclude that:
• The BB grows best in LB. • It grows less in Minimal Media. • It shows almost no growth in Saline Solution. • Il grows best @37°C • It grows good @23°C • It shows almost no growth @8°C
Experiments with BBa_K410000
 From these data we could conclude that:
• The BB grows best in LB. • It grows well in Minimal Media. • It shows almost no growth in Saline Solution. • Il grows best @37°C • It grows well @23°C (best in Minimal Media than LB) • It shows almost no growth @8°C
Experiments with BBa_K672000
 From these data we could conclude that:
• The BB grows best in LB. • It shows almost no growth in Minimal Media. • It shows almost no growth in Saline Solution. • Il grows best @37°C • It shows almost no growth @23°C, just in LB. • It shows almost no growth @8°C
 Graphs:Experiments 1, 2 & 3:
Other Experiments with Bristol:
 Discussion of ResultsAs we expected, our Renbo grew well at 37°C, grew regular under cold shock stress in LB (23°C) and did not grow at 8°C. Looking at the results for BBa_410000, it decreases the capability of BBa_381001, under a cold shock. From these results we could determine sources of errors during the experiments and learn from these for our further investigations. The next step in our project: |
