Team:Grenoble/Projet/Results

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<P>The RsmA system has a homologous in Escherichia Coli named CsrA. We know these two system are extremely closed on structural and functional sides. The most difference between this two regulation systems is on target regulon. For example, in Pseudomonas aeruginosa Rsma regulate many virulence genes as type III secretion system (anja Brencic and Stephen Lory). In Escherichia coli, RsmA homologous regulate metabolic network. Our goal is to integrate this translational regulation system in the toggle switch. We need to know whether it influence the bacteria’s life.</P><a href="https://static.igem.org/mediawiki/2011/4/46/Growth_cur_final.png"><img
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    <h1>Achievements</h1>
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<strong><b>Figure 1 RsmA influence on DH5α growth.</b> Bacteria from overnight culture were reseeding in rich medium supplemented with tetracycline at 20mg/ml and also IPTG at the concentration given in the legend.</strong><p>Figure 1 present growth curve of DH5α carries into a plasmid pVLT31 with or without rsmA and Natural RBS cloned downstream the Plac promoter. Two triads can be seen. The triad containing the strains with empty plasmid shows an upper growth curve compared to the second triad carries pVLT31-rsmA. But it’s important to say that the two groups start their growth not at the same value. That explains a time lag between these two groups. After normalization of all curves, no differences between these two kind of bacteria could be seen. We conclude that the RsmA overexpression hasn’t effects on the growth of bacteria.</P>
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<h2>Table of content</h2>
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<p>
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We describe here the modeling and experimental results obtained for each network module,  
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in order to validate our model and optimize the device: the toggle switch, the quorum sensing
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and the translational regulation system RsmA.  
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<ol>
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<li>Validation of the genetic network</li>
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<li>The essential role of rmsA regulation system</li>
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<li>Device optimization and specifications</li>
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</ol>
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In each part, we tried to speak about both experiment and modelling, in order to validate our model and optimized
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by simulation the device.<br/><br/>
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  <a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Toggle" ><img class="icon" src="https://static.igem.org/mediawiki/2011/2/20/Bouton_toggle.png"/></a>
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      <big><big><a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Toggle" class="menu">Validation of the network</a></big></big><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/Toggle#TS_QS">Toggle Switch and Quorum sensing behavior</a><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/Toggle#Validation">Validation of the model</a><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/Toggle#Dynamic">Dynamic study of the stability</a><br/><br/><br/>
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  <a href="https://2011.igem.org/Team:Grenoble/Projet/Results/rmsA" ><img class="icon" src="https://static.igem.org/mediawiki/2011/9/97/Bouton_regulation.png"/></a>
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<big><big><a href="https://2011.igem.org/Team:Grenoble/Projet/Results/rmsA" class="menu">The essential role of rsmA regulation system</a></big></big><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/rmsA#Necessity">Why do we need this system ?</a><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/rmsA#fha">Leader sequence characterization</a><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/rmsA#rsma">rsmA characterization</a><br/><br/><br/>
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  <a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Device" ><img class="icon" src="https://static.igem.org/mediawiki/2011/7/70/Bouton_general_modeling.png"/></a>
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<big><big><a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Device" class="menu">Device specificities and optimization</a></big></big><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/Device#Optimization">Optimization of the device</a><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/Device#Limit">Determination of the limit of quantification</a><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/Device#Statistic">Stochastic study for statistic determination of several device specificities</a><br/><br/><br/>
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  <a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Sensitivity" ><img class="icon" src="https://static.igem.org/mediawiki/2011/f/fd/Bouton_model_sensitivity.png"/></a>
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<big><big><a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Sensitivity" class="menu">Sensitivity to parameters study</a></big></big><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/Sensitivity#Robustness">Robustness of our system</a><br/>
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<a class="menu" href="https://2011.igem.org/Team:Grenoble/Projet/Results/Sensitivity#Mercury">Applicable to mercury</a><br/><br/><br/><br/>
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    </p>
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  <input type="button" value="< PREVIOUS <" onclick="document.location = '/Team:Grenoble/Projet/Results';" />
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  <select name="id" onchange="document.location = '/Team:Grenoble/Projet/Results' + this.options[this.selectedIndex].value ;">
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    <option value="#Content" selected="selected">Table of content</option>
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    <optgroup label="Toggle Switch" >
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                                    <option value="/Toggle#TS_QS" >Toggle Switch and Quorum Sensing</option>
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                                    <option value="/Toggle#Validation" >Validation of the model</option>
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                                    <option value="/Toggle#Dynamic" >Dynamic study of the stability</option>
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                            </optgroup>
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                            <optgroup label="Post-transcriptional regulation (RsmA)">
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                                    <option value="/rmsA#Necessity">Necessity of this system</option>
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                                    <option value="/rmsA#fha">Leader sequence characterization</option>
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                                    <option value="/rmsA#rsma">rsmA characterization</option>
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                            </optgroup>
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                            <optgroup label="Device">
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    <option value="/Device#Optimization" >Optimization of the device</option>
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    <option value="/Device#Limit">Determination of the limit of quantification</option>
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    <option value="/Device#Statistic">Statistic study of device specificities</option>
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    </optgroup>
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                            <optgroup label="Sensitivity to parameters">
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    <option value="/Sensitivity#Robustness">Robustness of the system</option>
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    <option value="/Sensitivity#Mercury">Applicable to mercury</option>
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Latest revision as of 03:20, 29 October 2011

Grenoble 2011, Mercuro-Coli iGEM


Achievements

Table of content

We describe here the modeling and experimental results obtained for each network module, in order to validate our model and optimize the device: the toggle switch, the quorum sensing and the translational regulation system RsmA.

  1. Validation of the genetic network
  2. The essential role of rmsA regulation system
  3. Device optimization and specifications
In each part, we tried to speak about both experiment and modelling, in order to validate our model and optimized by simulation the device.

Validation of the network
Toggle Switch and Quorum sensing behavior
Validation of the model
Dynamic study of the stability


The essential role of rsmA regulation system
Why do we need this system ?
Leader sequence characterization
rsmA characterization


Device specificities and optimization
Optimization of the device
Determination of the limit of quantification
Stochastic study for statistic determination of several device specificities


Sensitivity to parameters study
Robustness of our system
Applicable to mercury



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