Team:WHU-China/Project

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This is a template page. READ THESE INSTRUCTIONS.
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#overview
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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#modeling
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#design
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#results
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{|align="justify"
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:WHU-China_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:WHU-China_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:WHU-China | Team Example]]
 
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<!--- The Mission, Experiments --->
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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<div id="overview">
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!align="center"|[[Team:WHU-China|Home]]
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<img src="/wiki/images/6/65/Whu-Overview.png"/>
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!align="center"|[[Team:WHU-China/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=WHU-China Official Team Profile]
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!align="center"|[[Team:WHU-China/Project|Project]]
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!align="center"|[[Team:WHU-China/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:WHU-China/Modeling|Modeling]]
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!align="center"|[[Team:WHU-China/Notebook|Notebook]]
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!align="center"|[[Team:WHU-China/Safety|Safety]]
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!align="center"|[[Team:WHU-China/Attributions|Attributions]]
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<img style="position:absolute;top:50px;width:400px;left:20px;" src="/wiki/images/a/a4/Whu-project3.jpg"/>
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<div id="introsub" style="position:absolute;top:70px;width:520px;right:40px;font-size:15px;">
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&nbsp;&nbsp;&nbsp;&nbsp;Being born, growing, aging and embracing death, the life process that every creature must go through is just like a movie. However, as one of the simplest life forms, E.coli's life story is too evanescent and monotonous. How can we utilize E.coli to show more complicated processes, such as the growing and withering of a tree? </br>
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&nbsp;&nbsp;&nbsp;&nbsp; Numerous problems stand in the way to this goal. So we just take two steps towards this direction. Firstly, we only focus on one pixel of this picture. As time goes by, the color of this point changes periodically. This effect is achieved by a strain of E.coli working as an oscillator which can yield different kinds of pigment periodically with the help of a signal transformation system....
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<a href="https://2011.igem.org/Team:WHU-China/Project/Overview"><img style="position:absolute;top:380px;right:100px;width:80px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a>
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<img src="/wiki/images/1/16/Whu-Design.png"/>
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== '''Overall project''' ==
 
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Stepping into  the IGEM world for the first time, we studied a huge amount of previous  projects and found that light sensing bacteria kept arousing general interest  but remain an unsettled issue. Despite the great efforts made by previous teams, little progress was made in this area. Therefore we decide to make some  novel attempts.
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&nbsp;&nbsp;&nbsp;&nbsp;This summer we designed two projects: color film and oscillator. One is about time and another is about space.  The oscillator is to construct one strain colorful E.coli changing color periodically. The color film project is to construct three strains of E.coli which can form a colorful image. Here is the detailed design of these two projects.     
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<a href="https://2011.igem.org/Team:WHU-China/Project/Design"><img style="position:absolute;top:380px;right:100px;width:80px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a>
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Firstly, we plan  to establish inter-bacteria communication with light as the media. We designed  three separate systems and try to coordinate them: the oscillator system, the  signal transformation system, and the reaction system. After chemical signals  are generated by the oscillator system periodically, the transformation system  transforms them into light signals and the reaction system act differently  according to the signals. Compared with chemical signals, light signals have  several advantages: they can travel without liquid media and can be more easily  tested. In a word, we hope to construct a whole system in which different  strains of E.coli communicate with each other through three types of light. This is of great significance in automatically manipulating bacteria&rsquo;s  behaviors.
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<div id="modeling">
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<img src="/wiki/images/0/0d/Whu-Modeling.png"/>
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<a href="https://2011.igem.org/Team:WHU-China/Project/Modeling"><img style="position:absolute;top:380px;right:100px;width:80px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a>
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<img style="position:absolute;top:50px;width:420px;left:20px;" src="https://static.igem.org/mediawiki/2011/e/e3/Model-2.png"/>
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&nbsp;&nbsp;&nbsp;&nbsp;In the part of waving time , there is a two nodes system , using the method of the mathematic theory of the ODE function and the random analysis. We get our model.</br>
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&nbsp;&nbsp;&nbsp;&nbsp;To stimulate ,taking the fluctuations arising from the molecules into account, we need to resort to stochastic simulations. We use here the Gillespie algorithm to simulate a stochastic version of the model.Suppose the system size Ω=1000,then we can get the result of simulation...
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Secondly, we came  up with the idea of &ldquo;colorful E.film&rdquo; based on previous research of  bio-imaging. We hope to create a colorful film instead of a black-and-white one  by constructing three E.coli strains which can produce and secrete three  primary colors respectively in the presence of different lights. The  combination of various concentrations of the primary colors will yield a  colorful picture. Therefore, if we use a color picture as an input signal, the  output will be the copy of it on the plate. The whole system works as a camera.
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<div id="results">
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<img src="/wiki/images/3/3e/Whu-Results.png"/>
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<img style="position:absolute;top:50px;width:400px;left:20px;" src="https://static.igem.org/mediawiki/2011/9/9a/Shuangban.png"/>
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<a href="https://2011.igem.org/Team:WHU-China/Project/Results"><img style="position:absolute;top:350px;right:100px;width:80px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a>
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<div id="introsub" style="position:absolute;top:70px;width:520px;right:40px;font-size:15px;">
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<p>&nbsp;&nbsp;&nbsp;Blue light induced bacteria with part BBa_K560000, blue light sensing and reporting system. E.coli cultures from the same clone were grown in the dark under the blue light. Control were under the dark. Bacteria under blue light expressed little RFP and the bacterial colony did not turn red. Control bacterial colony turned red obviously..... </p>
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The two parts of  our project have several components in common, such as the light sensors and  pigment producers. And the key word of our project is light sensing. Currently,  we have already done pre-experiments and in the next period we will try our  best to make the system work. We hope that our colorful E.coli that weaves time  and space will shine in this year.
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We will keep  updating information about our experiments.
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<image src="/wiki/images/5/58/Whu-backgroundbottom.png"/>
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'''''Look forward to  your attention!'''''
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<div id="contact">
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<a href="https://2011.igem.org/wiki/index.php?title=Team:WHU-China/Project&action=edit"><img class="whucontact" style="position:relative;left:636px;top:38px;z-index:10;" src="/wiki/images/2/2a/Whu-Home_r1_c1.png"/></a>
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== Project Details==
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<a href="Mailto:gubrian890512@gmail.com"><img class="whucontact" style="position:relative;left:660px;top:38px;z-index:10;" src="/wiki/images/9/97/Whu-Home_r1_c3.png"/></a>
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<a href="https://igem.org/Main_Page"><img class="whucontact" style="position:relative;left:676px;top:38px;z-index:10;" src="/wiki/images/0/03/Whu-Home_r1_c5.png"/></a>
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=== System One_Oscillator===
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{{WHUcss}}
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=== System Two_Converter===
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=== System Three_Prodction Line===
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=== System Four_Color Film ===
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== Results ==
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Latest revision as of 03:10, 29 October 2011

    Being born, growing, aging and embracing death, the life process that every creature must go through is just like a movie. However, as one of the simplest life forms, E.coli's life story is too evanescent and monotonous. How can we utilize E.coli to show more complicated processes, such as the growing and withering of a tree?
     Numerous problems stand in the way to this goal. So we just take two steps towards this direction. Firstly, we only focus on one pixel of this picture. As time goes by, the color of this point changes periodically. This effect is achieved by a strain of E.coli working as an oscillator which can yield different kinds of pigment periodically with the help of a signal transformation system....

    This summer we designed two projects: color film and oscillator. One is about time and another is about space. The oscillator is to construct one strain colorful E.coli changing color periodically. The color film project is to construct three strains of E.coli which can form a colorful image. Here is the detailed design of these two projects.

    In the part of waving time , there is a two nodes system , using the method of the mathematic theory of the ODE function and the random analysis. We get our model.
    To stimulate ,taking the fluctuations arising from the molecules into account, we need to resort to stochastic simulations. We use here the Gillespie algorithm to simulate a stochastic version of the model.Suppose the system size Ω=1000,then we can get the result of simulation...

   Blue light induced bacteria with part BBa_K560000, blue light sensing and reporting system. E.coli cultures from the same clone were grown in the dark under the blue light. Control were under the dark. Bacteria under blue light expressed little RFP and the bacterial colony did not turn red. Control bacterial colony turned red obviously.....

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