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Team:Paris Bettencourt/Experiments/Lambda switch
From 2011.igem.org
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<li>We successfully BioBricked both the YFP:TetR (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K606025">BBa_K606025</a>) and the TetO Array (<a href="http://partsregistry.org/Part:BBa_K606026">BBa_K606026</a>) constructs and sent them to the registry</li> | <li>We successfully BioBricked both the YFP:TetR (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K606025">BBa_K606025</a>) and the TetO Array (<a href="http://partsregistry.org/Part:BBa_K606026">BBa_K606026</a>) constructs and sent them to the registry</li> | ||
<li>We integrated our construct both in <i>E.coli</i> and <i>B.subtilis</i></li> | <li>We integrated our construct both in <i>E.coli</i> and <i>B.subtilis</i></li> | ||
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<li>We've done <i>E.coli</i> to <i>B.subtilis</i> diffusion experiments (with negative results)</li> | <li>We've done <i>E.coli</i> to <i>B.subtilis</i> diffusion experiments (with negative results)</li> | ||
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<h2>Integration into <i>B. subtilis</i></h2> | <h2>Integration into <i>B. subtilis</i></h2> | ||
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| + | [[File:cIbsubtilis.jpg|center|Integration of cI (<partinfo>BBa_K606068</partinfo>)]] | ||
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Revision as of 03:00, 29 October 2011
Building the Lambda switch design
Abstract
Results for the YFP concentrator:
- We successfully BioBricked both the YFP:TetR (BBa_K606025) and the TetO Array (BBa_K606026) constructs and sent them to the registry
- We integrated our construct both in E.coli and B.subtilis
- We've done E.coli to B.subtilis diffusion experiments (with negative results)
Design overview
Integration into B. subtilis
