Team:IIT Madras/Dry lab/Modelling/Validation

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<p align="center"><u><i> <b>Table 1</b></i>: .</p>
<p align="center"><u><i> <b>Table 1</b></i>: .</p>
<p align="center"><img src="https://static.igem.org/mediawiki/2011/5/5a/Table001.jpg" align="middle" width="500" height="400" align="center"/></p><br/>
<p align="center"><img src="https://static.igem.org/mediawiki/2011/5/5a/Table001.jpg" align="middle" width="500" height="400" align="center"/></p><br/>
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<p align="center"><img src="https://static.igem.org/mediawiki/2011/e/eb/No_azide.jpg" align="middle" width="500" height="400" align="center"/></p>
 
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<p align="center"><u> <i><b>Figure 1</b><i>: Plot for % increase in growth due to Proteorhodopsin at varying glucose concentration in the absence of Azide.</u></p><br/>
 
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<p> <b>According to the model the following reactions showed major flux changes due to Proteorhodopsin :</b>
 
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<ol><li>'adentylate kinase GTP ' </li>
 
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<li>'adenosine hydrolase' </li>
 
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<li>'dihydroorotic acid menaquinone 8  </li>
 
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<li>'3 isopropylmalate dehydrogenase'  </li>
 
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<li>'psicoselysine transport via proton symport periplasm </li>
 
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<li>'purine nucleoside phosphorylase Deoxyadenosine '  </li>
 
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<li>'L threonine via sodium symport periplasm ' </li></ol>
 
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<u>Reaction Knockout Analysis of    '3 isopropylmalate dehydrogenase'  was lethal and  the other reactions didn’t have major effects on growth rates .</u></p><br/>
 
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<p align="center"><img src="https://static.igem.org/mediawiki/2011/8/8c/70p_inhib.jpg" align="middle" width="500" height="400" align="center"/></p>
<p align="center"><img src="https://static.igem.org/mediawiki/2011/8/8c/70p_inhib.jpg" align="middle" width="500" height="400" align="center"/></p>

Revision as of 02:37, 29 October 2011

bar iGEM 2011 - Home Page Indian Institute of Technology - Madras



Validation of Model


The validation was done with negative regulation of the cytochrome oxidase reaction by comparing with literature available for inhibition using azide[3]


Table 1: .


Figure 2: Plot for % increase in growth due to Proteorhodopsin at varying glucose concentration for 70% inhibition of Oxidative phosphorylation (ETC) on addition of azide.


According to the model the following reactions showed major flux changes due to Proteorhodopsin :

  1. 'adentylate kinase GTP '
  2. 'adenosine hydrolase'
  3. 'dihydroorotic acid menaquinone 8 '
  4. 'Glycolate oxidase' Needs to be done
  5. 'psicoselysine transport via proton symport periplasm '
  6. 'purine nucleoside phosphorylase Deoxyadenosine '
  7. 'L threonine via sodium symport periplasm '

Reaction Knockout Analysis of all the reactions didn’t have major effects on growth rates .



Figure 3: Plot for % increase in growth due to Proteorhodopsin at varying glucose concentration for complete inhibition of Oxidative phosphorylation (ETC) on addition of high concentration of azide.



According to the model the following reactions showed major flux changes due to Proteorhodopsin :

  1. adentylate kinase GTP
  2. adenosine hydrolase
  3. dihydroorotic acid menaquinone 8
  4. 3'isopropylmalatedehydrogenase'
  5. psicoselysine transport via proton symportperiplasm
  6. purine nucleoside phosphorylaseDeoxyadenosine
  7. L threonine via sodium symportperiplasm
Reaction Knockout Analysis of '3 isopropylmalate dehydrogenase' was lethal and the other reactions didn’t have major effects on growth rates .





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