Team:Grenoble/Projet/Design/quorum
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- | < | + | <h1 >The Quorum Sensing</h1> |
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- | If | + | If LacI pathway is activated, CinI proteins are expressed providing to the bacteria the ability to release in the medium Quorum Sensing molecules, AHL. |
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- | Whereas if | + | Whereas if MerR pathway is activated, CinR proteins are expressed in the inner bacteria. Thus, these bacteria will be able to received AHL molecule. |
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We make use of this complex to visualize the boundary between the two different bacterial behavior areas. | We make use of this complex to visualize the boundary between the two different bacterial behavior areas. | ||
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<img height="300" src="https://static.igem.org/mediawiki/2011/1/1b/Details_lycopene.png"/> | <img height="300" src="https://static.igem.org/mediawiki/2011/1/1b/Details_lycopene.png"/> | ||
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+ | Both of these parts of the genetic network are modelled in the <a href="https://2011.igem.org/Team:Grenoble/Projet/Modelling/Deterministic#Our_EquationsQS">same module</a>. | ||
+ | Coupled with the toggle switch module, this model gives us the behavior of <a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Toggle#QS">quorum sensing production and complexation</a>. | ||
+ | From which we can deduce some <a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Device#Optimization">optimizations</a> for our device. | ||
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+ | <form method="get" > | ||
+ | <input type="button" value="< PREVIOUS <" onclick="document.location = '/Team:Grenoble/Projet/Design/toggle';" /> | ||
+ | <select name="id" onchange="document.location = '/Team:Grenoble/Projet' + this.options[this.selectedIndex].value ;"> | ||
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+ | <option value="/Design/toggle" >The Toggle Switch</option> | ||
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+ | <optgroup label="Quorum Sensing"> | ||
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+ | <option value="/Design/quorum" selected="selected">The Quorum Sensing</option> | ||
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+ | <option value="/Design/quorum#coloration">The Coloration</option> | ||
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+ | {{:Team:Grenoble/Design/pied}} |
Latest revision as of 02:31, 29 October 2011
The Quorum Sensing
By coupling Toggle Switch with Quorum Sensing genes, the switch in a pathway defines a specific behavior: receiving or sending bacteria.
Put together, both bacterial behaviors allow the formation of AHL/CinR complex in the inner receiving bacteria.
![]() |
We make use of this complex to visualize the boundary between the two different bacterial behavior areas. |
![](https://static.igem.org/mediawiki/2011/3/3e/QS_details_bis.png)
Coloration
The two behaviors encounter each other at the boundary. At this location cells emit AHL closely to receivers.
The AHL molecules enter into the receiving cells and bind receptor protein to form a complex which up-regulates the promoter pCin. Thus, the expression of the three Lycopene synthesise enzymes is allowed. These three enzymes (crtB, crtE, crtI) produce the lycopene pigment so we can observe a red stripe at the interface between receiving and senders.
![](https://static.igem.org/mediawiki/2011/1/1b/Details_lycopene.png)
Both of these parts of the genetic network are modelled in the same module. Coupled with the toggle switch module, this model gives us the behavior of quorum sensing production and complexation. From which we can deduce some optimizations for our device.
![]() iGEM 2011 Main Page Contact Us:Clic here ! |
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