Team:Grenoble/Projet/Design/quorum

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<h1 >The Quorum Sensing</h1>
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By coupling Toggle Switch with Quorum Sensing genes, the switch in a pathway defines a specific behavior: receiving or sending bacteria.
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<a href="https://static.igem.org/mediawiki/2011/a/a3/Toggle_Switch.png"><img src="https://static.igem.org/mediawiki/2011/a/a3/Toggle_Switch.png"  width="300px"></a>
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<a href="http://2011..org/wiki/images/4/4f/TS_QS_send.png"><img src="https://static.igem.org/mediawiki/2011/4/4f/TS_QS_send.png"  width="450px"></a>
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If LacI pathway is activated, CinI proteins are expressed providing to the bacteria the ability to release in the medium Quorum Sensing molecules, AHL.
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<a href="https://static.igem.org/mediawiki/2011/3/37/TS_QS_recei.png"><img src="https://static.igem.org/mediawiki/2011/3/37/TS_QS_recei.png"  width="450px"></a>
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The Toggle Switch allows our bacterial strain to switch between one of the two different behaviors (Receiver or Secretor) depending on the predominant concentration in the external medium: IPTG or Hg.
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Whereas if MerR pathway is activated, CinR proteins are expressed in the inner bacteria. Thus, these bacteria will be able to received AHL molecule.
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We take into account two proteins repressing (CinI/CinR) each other's expression (pLac/pMerT). We create the fundaments of a toggle system that can switch when using two inducers in the medium: IPTG and Hg.
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Initially, both genes, MerR and LacI, are expressed repressing each other promoters: pLac and pMerT.
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Put together, both bacterial behaviors allow the formation of AHL/CinR complex in the inner receiving bacteria.
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On the plate where Hg concentration is the uppermost, this inducer will remove the repression exerted on pMerT promoter. So, the LacI proteins will be expressed and by repressing the promoter pLac, LacI proteins are shutting down the other way of the toggle.
 
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<a href="https://static.igem.org/mediawiki/2011/4/43/Toggle_Switch_predominance1.png"><img src="https://static.igem.org/mediawiki/2011/4/43/Toggle_Switch_predominance1.png"  width="300px"></a>
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<a href="https://static.igem.org/mediawiki/2011/3/36/Coloration_toi_toi_mon_toi.png"><img src="https://static.igem.org/mediawiki/2011/3/36/Coloration_toi_toi_mon_toi.png"  width="350px"></a>
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<a href="https://static.igem.org/mediawiki/2011/2/24/Toggle_Switch_predominance2.png"><img src="https://static.igem.org/mediawiki/2011/2/24/Toggle_Switch_predominance2.png"  width="300px"></a>
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Inversely, where IPTG concentration is the uppermost, the other way will be activated.
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We make use of this complex to visualize the boundary between the two different bacterial behavior areas.
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<img height="300" src="https://static.igem.org/mediawiki/2011/3/3e/QS_details_bis.png"/>
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<h2 id="color">Coloration</h2>
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<h2>Coloration</h2>
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The two populations encounter each other at the “interface”. At this location cells emit AHL closely to receivers.
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The two behaviors encounter each other at the boundary. At this location cells emit AHL closely to receivers.
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The AHL molecules enter into the receiving cells and bind receptor protein to form a complex to up-regulate the promoter pCin. Allowing the expression of the three Lycopene synthesise enzymes coding crtB, crtE, crtI. These three enzymes produce the lycopene so we can observe a red stripe at the interface between receiving and senders.  
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The AHL molecules enter into the receiving cells and bind receptor protein to form a complex which up-regulates the promoter pCin. Thus, the expression of the three Lycopene synthesise enzymes is allowed. These three enzymes (crtB, crtE, crtI) produce the lycopene pigment so we can observe a red stripe at the interface between receiving and senders.  
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<img height="300" src="https://static.igem.org/mediawiki/2011/1/1b/Details_lycopene.png"/>
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Both of these parts of the genetic network are modelled in the <a href="https://2011.igem.org/Team:Grenoble/Projet/Modelling/Deterministic#Our_EquationsQS">same module</a>.
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Coupled with the toggle switch module, this model gives us the behavior of <a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Toggle#QS">quorum sensing production and complexation</a>.
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From which we can deduce some <a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Device#Optimization">optimizations</a> for our device.
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Latest revision as of 02:31, 29 October 2011

Grenoble 2011, Mercuro-Coli iGEM


The Quorum Sensing

By coupling Toggle Switch with Quorum Sensing genes, the switch in a pathway defines a specific behavior: receiving or sending bacteria.

If LacI pathway is activated, CinI proteins are expressed providing to the bacteria the ability to release in the medium Quorum Sensing molecules, AHL.

Whereas if MerR pathway is activated, CinR proteins are expressed in the inner bacteria. Thus, these bacteria will be able to received AHL molecule.



Put together, both bacterial behaviors allow the formation of AHL/CinR complex in the inner receiving bacteria.



We make use of this complex to visualize the boundary between the two different bacterial behavior areas.

Coloration

The two behaviors encounter each other at the boundary. At this location cells emit AHL closely to receivers.

The AHL molecules enter into the receiving cells and bind receptor protein to form a complex which up-regulates the promoter pCin. Thus, the expression of the three Lycopene synthesise enzymes is allowed. These three enzymes (crtB, crtE, crtI) produce the lycopene pigment so we can observe a red stripe at the interface between receiving and senders.

Both of these parts of the genetic network are modelled in the same module. Coupled with the toggle switch module, this model gives us the behavior of quorum sensing production and complexation. From which we can deduce some optimizations for our device.

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