Team:WHU-China/Notebook

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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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<style type="text/css">
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This is a template page. READ THESE INSTRUCTIONS.
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#protocol
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#bottom
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{
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position:absolute;
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z-index:1;
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#daytoday
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height:400px;
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width:1000px;
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<body>
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<div id="content1">
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<div id="protocol">
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<img src="/wiki/images/6/6f/Whu-Protocols.png"/>
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<img src="https://static.igem.org/mediawiki/2011/c/c6/Whu-protologo1.png" style="position:absolute;left:20px;top:40px;width:300px;height:360px;">
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<div style="position:absolute;top:60px;right:30px;width:620px;font-size:15px;">
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•          Dealing with biobricks</br>
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•          Preparation of competent cells (Using Calcium Chloride)</br>
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•          Plasmid Extraction（Using Plasmid Mini Kit）</br>
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•          Transformation (heat-shock method)</br>
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•          Cleavage with two restriction enzymes</br>
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•          Get Extraction</br>
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•          Identification of DNA by Electrophoresis</br>
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•          Ligation</br>
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•          PCR of the colony</br>
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•          Identification of DNA by Sequencing</br>
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•          Others............
</div>
</div>
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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<a href="https://2011.igem.org/Team:WHU-China/Notebook/Protocols"><img style="position:absolute;top:380px;right:100px;width:80px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a>
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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</div>
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
 
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</div>
 
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</html>
 
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<div id="daytoday">
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<img src="/wiki/images/8/83/Whu-Progress.png"/>
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<img src="/wiki/images/0/02/Whu-Progress.jpg" style="position:absolute;left:20px;top:53px;width:400px;height:320px;">
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<div style="position:absolute;top:60px;right:30px;width:520px;font-size:15px;">
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The forth layer assembly and assembled CHL1-3:</br>
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&nbsp;&nbsp;&nbsp;&nbsp;8/26 extract plasmid: 009-13,009-3. Enzyme digest 009-13,009- 3. Result shows that it is not cut completely .PCR 20 + 2 positive control.Transformation: 2 M-13 J-2 M-20 F, PSB, 2 K-16 MIV, CHL1-CHL3 (DH5 α and TrpR defects strains)</br>
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&nbsp;&nbsp;&nbsp;&nbsp;8/27 extract plasmid: 8H, 2M-14 H-2M. enzyme digest 009-3, 8H, 2 M-14 H-2 M. Ligate 009-2-3.Identification of the transformation of PCR</br> &nbsp;&nbsp;&nbsp;&nbsp;8/26 ligation results. Cut CHL1 religate CHL1-CHL3, CHL1-CHL4.Enzyme cut PCB, 23 L, RLS1.Transformation: CHL1-CHL3 (DH5 α and TrpR defects plant), CHL1-CHL4 (DH5 α and TrpR defects plant), 009-2-3, controls.</br>
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&nbsp;&nbsp;&nbsp;&nbsp;8/28 repeat 27th enzymes digest,ligated 009-2-3. Transformation: 18 M-RLS1, 18 O-RLS1, 2 G Ⅱ-RLS1, PSB-23 L, 009-2-3, CHL1-CHL3 (TrpR defects plant), CHL1-CHL4 (TrpR defects strains) controls. Reserve  RLSF-2, PSB-1.....
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{|align="justify"
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</div>
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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<a href="https://2011.igem.org/Team:WHU-China/Notebook/Daytoday"><img style="position:absolute;top:380px;right:100px;width:80px;height:30px" src="/wiki/images/1/14/Whu-More.png "></a>
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|[[Image:WHU-China_logo.png|200px|right|frame]]
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</div>
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|-
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|
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:WHU-China_team.png|right|frame|Your team picture]]
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|-
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|align="center"|[[Team:WHU-China | Team Example]]
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|}
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<!--- The Mission, Experiments --->
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<div style="height:100px;"></div>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:WHU-China|Home]]
 
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!align="center"|[[Team:WHU-China/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=WHU-China Official Team Profile]
 
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!align="center"|[[Team:WHU-China/Project|Project]]
 
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!align="center"|[[Team:WHU-China/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:WHU-China/Modeling|Modeling]]
 
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!align="center"|[[Team:WHU-China/Notebook|Notebook]]
 
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!align="center"|[[Team:WHU-China/Safety|Safety]]
 
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!align="center"|[[Team:WHU-China/Attributions|Attributions]]
 
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|}
 
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<div id="bottom">
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==Notebook==
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<image src="/wiki/images/5/58/Whu-backgroundbottom.png"/>
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</div>
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
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<div id="contact">
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<a href="https://2011.igem.org/wiki/index.php?title=Team:WHU-China/Notebook&action=edit"><img class="whucontact" style="position:relative;left:636px;top:38px;z-index:10;" src="/wiki/images/2/2a/Whu-Home_r1_c1.png"/></a>
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<a href="Mailto:gubrian890512@gmail.com"><img class="whucontact" style="position:relative;left:660px;top:38px;z-index:10;" src="/wiki/images/9/97/Whu-Home_r1_c3.png"/></a>
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<a href="https://igem.org/Main_Page"><img class="whucontact" style="position:relative;left:676px;top:38px;z-index:10;" src="/wiki/images/0/03/Whu-Home_r1_c5.png"/></a>
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<img id="bb" src="/wiki/images/3/3c/Whu-build.jpg"/>
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</div>
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</div>
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</body>
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</html>
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{{WHUcss}}

Latest revision as of 02:17, 29 October 2011

• Dealing with biobricks
• Preparation of competent cells (Using Calcium Chloride)
• Plasmid Extraction（Using Plasmid Mini Kit）
• Transformation (heat-shock method)
• Cleavage with two restriction enzymes
• Get Extraction
• Identification of DNA by Electrophoresis
• Ligation
• PCR of the colony
• Identification of DNA by Sequencing
• Others............
The forth layer assembly and assembled CHL1-3:
    8/26 extract plasmid: 009-13,009-3. Enzyme digest 009-13,009- 3. Result shows that it is not cut completely .PCR 20 + 2 positive control.Transformation: 2 M-13 J-2 M-20 F, PSB, 2 K-16 MIV, CHL1-CHL3 (DH5 α and TrpR defects strains)
    8/27 extract plasmid: 8H, 2M-14 H-2M. enzyme digest 009-3, 8H, 2 M-14 H-2 M. Ligate 009-2-3.Identification of the transformation of PCR
    8/26 ligation results. Cut CHL1 religate CHL1-CHL3, CHL1-CHL4.Enzyme cut PCB, 23 L, RLS1.Transformation: CHL1-CHL3 (DH5 α and TrpR defects plant), CHL1-CHL4 (DH5 α and TrpR defects plant), 009-2-3, controls.
    8/28 repeat 27th enzymes digest,ligated 009-2-3. Transformation: 18 M-RLS1, 18 O-RLS1, 2 G Ⅱ-RLS1, PSB-23 L, 009-2-3, CHL1-CHL3 (TrpR defects plant), CHL1-CHL4 (TrpR defects strains) controls. Reserve RLSF-2, PSB-1.....
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