Team:ETH Zurich

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|style="font-size:2em; height: 30px" class="overview" valign="top"|Smoke Sensors
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|align="center"|[[File:Design2.png|380px|center|link=Team:ETH_Zurich/Overview/CircuitDesign]] '''Sensor Design 1:''' Acetaldehyde Sensor
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[[File:Alternative_Xylene_system.png|380px|center|link=Team:ETH_Zurich/Overview/CircuitDesign]] '''Sensor Design 2:''' Xylene Sensor
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|style="font-size:2em; height: 30px" class="overview"|Abstract
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|valign="top" style="height:100%"|'''SmoColi is a bacterio-quantifier of smoke concentration that can be used as a passive smoke detector. Cigarette smoke contains a lot of different toxic and carcinogenic substances, most of which are volatile, e.g. acetaldehyde or xylene. Therefore we can use them as an airborne information carrier. The SmoColi cells are immobilized in an agarose-filled microfluidic device. The test solution is fed to one end of a microfluidic channel, in which a concentration gradient is established by diffusion and synthetic cellular degradation. The cells are engineered to sense a certain molecule. Some sensors were integrated as found in nature, others had to by synthetically re-designed, e.g. the fungal acetaldehyde-responsive transactivator. The sensor is linked to a band-pass filter that drives GFP expression. This allows establishment of an input-concentration-dependent, moving fluorescent band displaying quantitative information of the input. Finally, if the input concentration exceeds the threshold of malignance, a quorum-sensing-based mCherry alarm system springs into action, turning the entire device red.'''
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|style="font-size:2em; text-align: center" class="overview"|[[Team:ETH_Zurich/Achievements/Data_page|Data Page]]
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|style="font-size:2em; text-align: center" class="overview"|[[Team:ETH_Zurich/Biology/Detector|Network Elements]]
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|style="font-size:2em; text-align: center" class="overview"|[[Team:ETH_Zurich/Process/Validation|Microfluidics Evaluation]]
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|style="font-size:2em; text-align: center" class="overview"|[[Team:ETH_Zurich/Modeling/Combined|Combined Model]]
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|style="font-size:2em; height: 30px" class="overview" valign="top"|Team
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|valign="top"|[[File:GroupETH_irena_brightened_sky_darkened_auto_corrected.jpg|246px|center|The SmoColi Team|link=Team:ETH_Zurich/Team]]
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|style="font-size:2em; height: 30px" class="overview" valign="top"|Signal Processing by SmoColi
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|valign="top" style="text-align: center"|[[File:ETHZ-Overview_Information_Processing.png|600px|center|Overview of our system|link=Team:ETH_Zurich/Overview/CircuitDesign]]
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'''Overview of the information processing steps in SmoColi'''
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|style="font-size:2em; height: 30px" class="overview"|Our Sponsors
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<html>
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<a href="http://www.basf.ch" target="_blank"></html>[[File:ETHZ-BASF.png|220px|link=]]<html>
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</a>&nbsp;&nbsp;
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<a href="http://www.ethz.ch" target="_blank"></html>[[File:ETH_Zurich_Logo.png|220px|link=]]<html>
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</a>&nbsp;&nbsp;
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<a href="http://www.lonza.com" target="_blank"></html>[[File:ETHZ-Lonza.png|220px|link=]]<html>
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</a>&nbsp;&nbsp;
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<a href="http://www.merckserono.com" target="_blank"></html>[[File:ETHZ-Merck_Serono.png|250px|link=]]<html></a>&nbsp;&nbsp;
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<a href="http://www.novartis.ch" target="_blank"></html>[[File:ETHZ-Novartis.png|220px|link=]]<html>
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</a>&nbsp;&nbsp;
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<a href="http://www.roche.ch" target="_blank"></html>[[File:ETHZ-Roche.png|220px|link=]]<html>
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</a>&nbsp;&nbsp;
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<a href="http://www.syngenta.com" target="_blank"></html>[[File:ETHZ-Syngenta.png|220px|link=]]<html>
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</a>&nbsp;&nbsp;
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<a href="http://www.dsm.com" target="_blank"></html>[[File:DSM MasterLogo.png|220px|link=]]<html>
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= SmoColi - Abstract =
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'''We create a bacterio-quantifier of smoke. SmoColi cells are engineered to sense toxic substances found in cigarette smoke (for example acetaldehyde or xylene). They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red. Due to its modularity, our SmoColi system can be used in fact as a quantifier for a range of substances, as long as the sensor is adapted. To show that our system can be activated by different signals, as a proof of principle, we modified the circuit so that it can be induced by arabinose.'''
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Latest revision as of 01:31, 29 October 2011

Can you feel the smoke tonight?
 

Contents

SmoColi - Abstract

We create a bacterio-quantifier of smoke. SmoColi cells are engineered to sense toxic substances found in cigarette smoke (for example acetaldehyde or xylene). They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red. Due to its modularity, our SmoColi system can be used in fact as a quantifier for a range of substances, as long as the sensor is adapted. To show that our system can be activated by different signals, as a proof of principle, we modified the circuit so that it can be induced by arabinose.

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