Team:UC Davis

From 2011.igem.org

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<h1>Welcome</h1>
<h1>Welcome</h1>
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<p>Welcome to the home of the 2011 iGEM Team at UC Davis!<br><br>
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<p><a href="http://farm7.static.flickr.com/6159/6191041172_ae7b9bb9c9_b.jpg"><img src="http://farm7.static.flickr.com/6159/6191041172_ae7b9bb9c9_b.jpg" width="400" align="left"></a>
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The four of us have been hard at work on this year's project. Things are coming along well -- we've developed a powerful (and simple) mutagenic PCR protocol, used it on GFP, created and screened a set of LacI promoter mutants, and have selected several TetR and c1 Lambda promoter mutants for characterization.<br><br>
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Welcome to iGEM 2011 at UC Davis!<br><br>
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We are excited for the regional competition in Indianapolis, and are in the process of tidying up the wiki and finalizing our current data for presentation. If you see broken pages (especially ones that aren't fixed within 30 minutes of viewing), please feel free to email us at ucdigem2011@gmail.com.</p>
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The four of us have been hard at work on this year's project. We've fine tuned a powerful (and simple) part family generation process, and used it to expand the functionality of the LacI promoter, R0010. We also mutated GFP!<br><br>
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In addition, we developed <a href="https://2011.igem.org/Team:UC_Davis/KO3D">KO3D</a>, an interactive, cross-platform javascript plotting library for displaying scientific data on the web in 3D. <br><br>
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We had a great time at the regional competition in Indianapolis and look forward to seeing all of the other teams at the World Championship at MIT!
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<h1 id=Project>Our Project</h1>  
<h1 id=Project>Our Project</h1>  
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<p>This is the third time a team at UC Davis has competed in the iGEM Jamboree. This year, we are focusing on foundational advances for <a href="http://biobricks.org/" target="_blank">BioBrick</a> circuit synthesis. Our project revolves around the streamlining of a <a href="https://2011.igem.org/wiki/index.php?title=Team:UC_Davis/Protocols#ER-PCR">mutagenic PCR protocol</a> for the synthesis of mutant libraries from any BioBrick part, from promoters to protein coding genes.<br><br>
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<p>This is the third time a team at UC Davis has competed in the iGEM Jamboree. This year, we are focusing on foundational advances for <a href="http://biobricks.org/" target="_blank">BioBrick</a> circuit synthesis. Part families, groups of parts with a unified function and differeing characteristics, are some of the most useful parts of the registry. Our project aims to streamline the process of expanding basic parts into part families, from promoters to protein-coding genes.
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Using this protocol, we set out to create useful mutant libraries of commonly used parts, starting with the repressible LacI, TetR and Lambda c1 promoters. You can read more about our project <a href="https://2011.igem.org/Team:UC_Davis/Project">here.</a><br><br>
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<img src="http://farm7.static.flickr.com/6194/6084162068_09cbe08d11_b.jpg" width="250" align="left"></a>We set out to create useful mutant libraries of commonly used parts, starting with the repressible LacI promoter. You can read more about our project<a href="https://2011.igem.org/Team:UC_Davis/Project"> here.</a><br><br>
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We think that thorough characterization should be a priority for all parts submitted to the <a href="http://partsregistry.org" target="_blank">registry</a>. You can view information about our project on our <a href="https://2011.igem.org/Team:UC_Davis/Project">project</a> page and review our characterization process and more information on our constructions (say, for <a href="https://2011.igem.org/Team:UC_Davis/Data_LacI">LacI</a>) on our <a href="https://2011.igem.org/Team:UC_Davis/Data">data</a> page.<br><br>
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We think that thorough characterization should be a priority for all parts submitted to the <a href="http://partsregistry.org" target="_blank">registry</a>. You can view information about our project<a href="https://2011.igem.org/Team:UC_Davis/Project"> here</a>. You can also review our characterization process and <a href="https://2011.igem.org/Team:UC_Davis/Data_LacI">characterization data for LacI</a> on our <a href="https://2011.igem.org/Team:UC_Davis/Data">data</a> page.<br><br>
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The <a href="https://2011.igem.org/Team:UC_Davis/Team">team</a> page has information on the <a href="https://2011.igem.org/Team:UC_Davis/Team#Team">four undergrads</a> that make up this year's team and their advisors, as well as <a href="https://2011.igem.org/Team:UC_Davis/Team#Contact">contact information</a> for getting in touch with us.<br><br>
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Our <a href=""https://2011.igem.org/Team:UC_Davis/Safety">safety</a> page discusses the potential impact of our project on <a href=https://2011.igem.org/Team:UC_Davis/Safety#Environmental">environmental</a>, <a href="https://2011.igem.org/Team:UC_Davis/Safety#Public">public</a>, and <a href="https://2011.igem.org/Team:UC_Davis/Safety#Researcher">researcher</a> safety. It also contains our answers to the iGEM 2011 <a href="https://2011.igem.org/Team:UC_Davis/Safety#Questionnaire"> safety questionnaire.</a><br><br>
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The <a href="https://2011.igem.org/Team:UC_Davis/Notebook">notebook</a> page contains daily updates about our progress in lab, and also has a <a href="https://2011.igem.org/Team:UC_Davis/Gallery">gallery</a> where you can view pictures of our lab.<br><br>
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Credit for the various facets of our project, from <a href="https://2011.igem.org/Team:UC_Davis/Attributions#Wet">wetlab work</a> to <a href="https://2011.igem.org/Team:UC_Davis/Attributions#Wiki">graphic design</a> can be found on our <a href="https://2011.igem.org/Team:UC_Davis/Attributions">attributions</a> page, along with a list of the <a href="https://2011.igem.org/Team:UC_Davis/Attributions#opensource">awesome, free, open-source software</a> that we used to make this project happen.
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<h2>Make Your Own Part Family</h2>
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Our <a href="https://2011.igem.org/Team:UC_Davis/PartFamilies">part family expansion process</a> turns basic parts into part families. Check out how easy it is to improve your favorite BioBricks! You can also listen to Nick and Aaron discuss the process on our <a href="http://www.soundcloud.com/igem-at-uc-davis/part-families-rock">Soundcloud</a> page.
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<h2>Team</h2>
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The <a href="https://2011.igem.org/Team:UC_Davis/Team">team</a> page has information on the four undergrads that make up this year's team, their advisors, as well as contact information for getting in touch with us.<br><br><br>
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<h2>Safety</h2>
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<h1>Notebook</h1>
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Our <a href="https://2011.igem.org/Team:UC_Davis/Safety">safety</a> page discusses the potential impact of our project on environmental, public, and researcher safety. It also contains our answers to the iGEM 2011 <a href="https://2011.igem.org/Team:UC_Davis/Safety#Questionnaire"> safety questionnaire.</a><br><br>
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See what we did on a daily basis in lab. In here you'll find a detailed description of each day's work.<a href="https://2011.igem.org/Team:UC_Davis/Notebook" style="color:#33FF33"> Read more.</a><br><br></p>
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Take a look at some of the photos we've taken around the lab in our <a href="https://2011.igem.org/Team:UC_Davis/Gallery" style="color:#33FF33">gallery.</a><br><br>
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Want to know how we did our wet work?  You can see our protocols <a href="https://2011.igem.org/Team:UC_Davis/Protocols" style="color:#33FF33">here.</a><br><br></div><br><br>
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<h2>Notebook</h2>
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<h1>Attributions</h1>
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The <a href="https://2011.igem.org/Team:UC_Davis/Notebook">notebook</a> page contains daily updates about our progress in lab, and also has a <a href="https://2011.igem.org/Team:UC_Davis/Gallery">gallery</a> where you can view pictures that we've taken in our lab.<br><br><br>
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We've found that there is an inverse relationship between number of team members and amount of work.  For a breakdown of what we worked on, check out our <a href="https://2011.igem.org/Team:UC_Davis/Attributions" style="color:#33FF33">Attributions page.</a></p><br clear="all">
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<h2>Attributions</h2>
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Credit for the various facets of our project, from wetlab work to graphic design can be found on our <a href="https://2011.igem.org/Team:UC_Davis/Attributions">attributions</a> page, along with a list of the awesome, free, open-source software that we used to make this project happen.
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<h2>Safety</h2>
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For more information on how we worked safely in our lab, please visit our <a href="https://2011.igem.org/Team:UC_Davis/Safety" style="color:#33FF33">Safety page.</a>
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Latest revision as of 23:54, 28 October 2011

Our Sponsors

Start a Family

Got a favorite BioBrick? Check our our process for expanding basic parts into part families.

Criteria

View our judging criteria for iGEM 2011 here.

Welcome

Welcome to iGEM 2011 at UC Davis!

The four of us have been hard at work on this year's project. We've fine tuned a powerful (and simple) part family generation process, and used it to expand the functionality of the LacI promoter, R0010. We also mutated GFP!


In addition, we developed KO3D, an interactive, cross-platform javascript plotting library for displaying scientific data on the web in 3D.

We had a great time at the regional competition in Indianapolis and look forward to seeing all of the other teams at the World Championship at MIT!

Our Project

This is the third time a team at UC Davis has competed in the iGEM Jamboree. This year, we are focusing on foundational advances for BioBrick circuit synthesis. Part families, groups of parts with a unified function and differeing characteristics, are some of the most useful parts of the registry. Our project aims to streamline the process of expanding basic parts into part families, from promoters to protein-coding genes. We set out to create useful mutant libraries of commonly used parts, starting with the repressible LacI promoter. You can read more about our project here.

We think that thorough characterization should be a priority for all parts submitted to the registry. You can view information about our project here. You can also review our characterization process and characterization data for LacI on our data page.

Make Your Own Part Family

Our part family expansion process turns basic parts into part families. Check out how easy it is to improve your favorite BioBricks! You can also listen to Nick and Aaron discuss the process on our Soundcloud page.

Team

The team page has information on the four undergrads that make up this year's team, their advisors, as well as contact information for getting in touch with us.


Safety

Our safety page discusses the potential impact of our project on environmental, public, and researcher safety. It also contains our answers to the iGEM 2011 safety questionnaire.

Notebook

The notebook page contains daily updates about our progress in lab, and also has a gallery where you can view pictures that we've taken in our lab.




Attributions

Credit for the various facets of our project, from wetlab work to graphic design can be found on our attributions page, along with a list of the awesome, free, open-source software that we used to make this project happen.