Team:Queens Canada/Parts/Registry

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<regulartext>
<regulartext>
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     <span class="classpurple"><a href="#overview">GPCRs      </a></span>    </regulartext>
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     <span class="classpurple"><a href="#GPCR">GPCRs      </a></span>    </regulartext>
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<div id="space2">
<div id="space2">
<regulartext>
<regulartext>
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     <span class="classpurple"><a href="#mechanism"> chemotaxis </a></span>    </regulartext>
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     <span class="classpurple"><a href="#worm"> worm regulatory </a></span>    </regulartext>
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<div id="space3">
<div id="space3">
<regulartext>
<regulartext>
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     <span class="classpurple"><a href="#protocol">  composite  </a></span>    </regulartext>
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     <span class="classpurple"><a href="#composite">  composite  </a></span>    </regulartext>
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<div id="space4">
<div id="space4">
<regulartext>
<regulartext>
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     <span class="classpurple"><a href="#applications"> complete list    </a></span>    </regulartext>
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     <span class="classpurple"><a href="#table"> complete list    </a></span>    </regulartext>
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<div id="rightcontenttext">
<div id="rightcontenttext">
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<a name="GPCR"></a>
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<h3purple> Modular GPCR Constructs </h3purple><p>
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<h3purple>GPCR Constructs & Other Modular Parts </h3purple><p>
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<regulartext><b>Regulatory:</b></regulartext><br>
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<regulartext>In approaching our chemotaxis project, several G-protein coupled receptors were cultivated from multiple mammalian sources. </regulartext><br>
<br>
<br>
-
<regulartext><b>BBa_K631035 - Str2:</b> A promoter for AWC neurons, normally used for a 7-transmembrane olfactory receptor. We can use this as a promoter to integrate non-native GPCRs into the AWC neuron membrane.</regulartext><br>
 
-
<regulartext><b>BBa_K631001 - ODR-7:</b> A promoter for a 7-transmembrane olfactory receptor in AWA olfactory neurons. This can be used for integrating non-native GPCRs. Length: 1503</regulartext><br>
 
-
<regulartext><b>BBa_K631002 - let-858:</b> A promoter for a protein essential for the development of the worm. We used this in one of our composite parts to express fluorescence in the worm. </regulartext><br>
 
-
<regulartext><b>BBa_K631003 - GPC-1:</b> A promoter for one of the gamma sub-units of the GPCR binding proteins. We used this for our fluorescence as well. Length: 2000</regulartext><br>
 
-
<regulartext><b>BBa_K631004 - GCY7:</b> A promoter for a membrane guanylyl cyclase protein. This can be used for a non-native receptor or fluorescence reporter.</regulartext><br>
 
-
<br>
 
-
<regulartext><b>Coding:</b></regulartext><br>
 
-
<br>
 
-
<regulartext><b>  BBa_K631039 - nahD:</b> The coding sequence for the nahD protein, from P. peutida, capable of degrading napthalene. </regulartext><br>
 
-
<regulartext><b>  BBa_K631005 - NPY1R_Mus:</b>The coding sequence for the NPY1R protein. This is a GPCR from <i>Mus musculus</i>.</regulartext><br>
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<div id="photos">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/1/1d/Queens_CanadaArrow.png" >
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/1/1d/Queens_CanadaArrow.png" >
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/1/1d/Queens_CanadaArrow.png" >
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/1/1d/Queens_CanadaArrow.png" >
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/1/1d/Queens_CanadaArrow.png" >
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/1/1d/Queens_CanadaArrow.png" >
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</div>
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<regulartext><b> BBa_K631006 - NPY1R_Rat:</b>The coding sequence for the NPY1R protein from <i>Rattus norvegius</i>. This is also a GPCR .</regulartext><br>
 
-
<regulartext><b>BBa_K631007 - ADBR2:</b> The coding sequence for ADBR2, a mouse GPCR that binds phenol (albuteroll). Length: 1257 </regulartext><br>
 
 +
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631005">BBa_K631005- NPY1R_Mus  </a></span><p>
 +
<regulartext>The coding sequence for the NPY1R protein. This is a GPCR from <i>Mus musculus</i>.</regulartext>
 +
</div>
-
<regulartext><b>BBa_K631009 - OX1R:</b> Coding sequence for OX1R, a mouse GPCR that binds cholorobenzoic acid. </regulartext><br>
 
-
<regulartext><b> BBa_K631010 - MARM:</b> Coding sequence for MARM1, a rat receptor that binds phenol (hydroquinone).</regulartext><br>
+
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631006">BBa_K631006 - NPY1R_Rat </a></span><p>
 +
<regulartext>The coding sequence for the NPY1R protein from <i>Rattus norvegius</i>. This is also a GPCR .</regulartext><br>
 +
</div>
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<br>
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<div id="description">
-
<regulartext><b>Reporter:</b></regulartext><br>
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<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631007">BBa_K631007 - ADBR2 </a></span><p>
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<br>
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<regulartext>The coding sequence for ADBR2, a mouse GPCR that binds phenol (albuteroll). Length: 1257 </regulartext><br>
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<regulartext><b> BBa_K631013 - RFP:</b> Red fluorescent protein for the reporter system in our worm.</regulartext><br>
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</div>
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<regulartext><b> BBa_K631036 - eCFP:UNC-54:</b> Eukaryotic cyano-fluorescent protein for the reporter system in our worm. Length: 1629.</regulartext><br>
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 +
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631009">BBa_K631009 - OX1R </a></span><p>
 +
<regulartext>Coding sequence for OX1R, a mouse GPCR that binds 4-cholorobenzoic acid. </regulartext><br>
 +
</div>
 +
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631010">BBa_K631010 - MARM1 </a></span><p>
 +
<regulartext>Coding sequence for MARM1, a rat receptor that binds phenol (hydroquinone).</regulartext><br>
</div>
</div>
 +
 +
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631039">BBa_K631039 - nahD </a></span><p>
 +
<regulartext>The coding sequence for the nahD protein, from <i>pseudomonas peutida</i>, capable of degrading napthalene. Expressing this in our worm may give it the ability to degrade naphthalene.</regulartext><br>
 +
</div>
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<div id="goright">
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<span class="classpurple"><a href="#top">back to top</a><span>
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</div>
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<div id="rightcontenttext">
<div id="rightcontenttext">
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<a name="worm"></a>
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<h3purple>Regulatory Constructs for <i> C. elegans </i></h3purple><p>
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<h3purple> Chemotaxis Constructs: Targeted Movement </h3purple><p>
 
-
<regulartext><b>  BBa_K631031 - ODR7:OX1R:UNC-54:</b> A composite part designed to place the non-native OX1R into the neurons of the worm using the ODR-7 promoter for the ability to detect cholorobenzoic acid.</regulartext><br>
 
-
<regulartext><b>BBa_K631032 - ODR7:ADBR2:UNC-54:</b> A composite part designed to place the non-native ADBR2 into the neurons of the worm using the ODR-7 promoter for the ability to detect phenol (albuteroll). Length: 3666 </regulartext><br>
 
-
<regulartext><b>BBa_K631033 - GCY7:ADBR2:UNC-54:</b> Another composite part with ADBR2, under the GCY7 promoter. </regulartext><br>
 
-
</regulartext> </regulartext>
 
 +
 +
<div id="photos">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/2/2d/Queens_CanadaPromoter.png">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/2/2d/Queens_CanadaPromoter.png">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/2/2d/Queens_CanadaPromoter.png">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/2/2d/Queens_CanadaPromoter.png">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/2/2d/Queens_CanadaPromoter.png">
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</div>
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<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631035">BBa_K631035 - Str2  </a></span><p>
 +
<regulartext> A promoter for AWC neurons, normally used for a 7-transmembrane olfactory receptor. We can use this as a promoter to integrate non-native GPCRs into the AWC neuron membrane.</regulartext><br>
</div>
</div>
 +
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631001">BBa_K631001 - ODR-7  </a></span><p>
 +
<regulartext>A promoter for a 7-transmembrane olfactory receptor in AWA olfactory neurons. This can be used for integrating non-native GPCRs. Length: 1503</regulartext><br>
 +
</div>
 +
 +
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631002">BBa_K631002 - let-858 </a></span><p>
 +
<regulartext>A promoter for a protein essential for the development of the worm. We used this in one of our composite parts to express fluorescence in the worm. </regulartext><br>
</div>
</div>
 +
 +
 +
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631003">BBa_K631003 - GPC-1  </a></span><p>
 +
<regulartext>A promoter for one of the gamma sub-units of the GPCR binding proteins. We used this for our fluorescence as well. Length: 2000</regulartext><br>
 +
</div>
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 +
 +
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631004">BBa_K631004 - GCY7  </a></span><p>
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<regulartext> A promoter for a membrane guanylyl cyclase protein. This can be used for a non-native receptor or fluorescence reporter.</regulartext><br>
 +
</div>
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<div id="goright">
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<span class="classpurple"><a href="#top">back to top</a><span>
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</div>
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</div>
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<div id="rightcontenttext">
<div id="rightcontenttext">
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<h3purple> Other Composite Constructs  </h3purple><p>
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<a name="composite"></a>
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<h3purple> Composite Constructs  </h3purple><p>
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<regulartext><b>BBa_K631037 - GPC-1:eCFP:UNC-54:</b> This is a composite part for our reporter system using cyano-fluorescent protein under the GPC-1 promoter. Length: 3787  </regulartext><br>
 
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<regulartext><b>  BBa_K631034 - let858:RFP:UNC-54:</b>This is a composite part for our reporter system using red fluorescent protein under the let858 promoter.</regulartext><br>
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<div id="photos">
 +
<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/6/6b/Queens_canadaBoxes.png">
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  <img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/6/6b/Queens_canadaBoxes.png">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/6/6b/Queens_canadaBoxes.png">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/6/6b/Queens_canadaBoxes.png">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/6/6b/Queens_canadaBoxes.png">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/6/6b/Queens_canadaBoxes.png">
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<img align="left" style="margin-bottom:8px; width:175px; padding:0;" src="https://static.igem.org/mediawiki/2011/6/6b/Queens_canadaBoxes.png">
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</div>
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<regulartext><b> BBa_K631038 - GCY-7:eCFP:UNC-54:</b> This is a composite part for our reporter system using cyano-fluorescent protein under the GCY-7 promoter.</regulartext><br>
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<div id="description">
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</regulartext> </regulartext> <p>
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<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631031">BBa_K631031 - ODR7:OX1R:UNC-54 </a></span><p>
 +
<regulartext>A composite part designed to place the non-native OX1R into the neurons of the worm using the ODR-7 promoter for the ability to detect cholorobenzoic acid.</regulartext><br>
 +
 
 +
</div>
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 +
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631032">BBa_K631032 - ODR7:ADBR2:UNC-54  </a></span><p>
 +
<regulartext>A composite part designed to place the non-native ADBR2 into the neurons of the worm using the ODR-7 promoter for the ability to detect phenol (albuteroll). Length: 3666 </regulartext><br>
 +
 
 +
</div>
-
<regulartext><b> BBa_K631039 - nahD:</b> The coding sequence for the nahD protein, from <i>pseudomonas peutida</i>, capable of degrading napthalene. Expressing this in our worm may give it the ability to degrade naphthalene.</regulartext><br>
+
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631033">BBa_K631033 - GCY7:ADBR2:UNC-54  </a></span><p>
 +
<regulartext> Another composite part with ADBR2, under the GCY7 promoter. </regulartext><br>
</regulartext> </regulartext>
</regulartext> </regulartext>
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</div>
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<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631037">BBa_K631037 - GPC-1:eCFP:UNC-54 </a></span><p>
 +
<regulartext>This is a composite part for our reporter system using cyano-fluorescent protein under the GPC-1 promoter. Length: 3787  </regulartext><br>
</div>
</div>
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<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631034">BBa_K631034 - let858:RFP:UNC-54  </a></span><p>
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<regulartext>This is a composite part for our reporter system using red fluorescent protein under the let858 promoter.</regulartext><br>
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</div>
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<div id="rightcontenttext">
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<div id="description">
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<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631038">BBa_K631038 - GCY-7:eCFP:UNC-54  </a></span><p>
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<regulartext> This is a composite part for our reporter system using cyano-fluorescent protein under the GCY-7 promoter.</regulartext><br>
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</div>
 +
<div id="description">
 +
<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631036">BBa_K631036 - eCFP:UNC-54  </a></span><p>
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<regulartext> Eukaryotic cyano-fluorescent protein for the reporter system in our worm. Length: 1629.</regulartext><br>
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</regulartext> </regulartext>
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<a name="table"></a>
<h3purple>Complete List of parts</h3purple><p>
<h3purple>Complete List of parts</h3purple><p>
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<div style="float:right;">
<groupparts>iGEM011 Queens_Canada</groupparts>
<groupparts>iGEM011 Queens_Canada</groupparts>
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Latest revision as of 23:45, 28 October 2011

GPCR Constructs & Other Modular Parts

In approaching our chemotaxis project, several G-protein coupled receptors were cultivated from multiple mammalian sources.

BBa_K631005- NPY1R_Mus

The coding sequence for the NPY1R protein. This is a GPCR from Mus musculus.

BBa_K631006 - NPY1R_Rat

The coding sequence for the NPY1R protein from Rattus norvegius. This is also a GPCR .

BBa_K631007 - ADBR2

The coding sequence for ADBR2, a mouse GPCR that binds phenol (albuteroll). Length: 1257

BBa_K631009 - OX1R

Coding sequence for OX1R, a mouse GPCR that binds 4-cholorobenzoic acid.

BBa_K631010 - MARM1

Coding sequence for MARM1, a rat receptor that binds phenol (hydroquinone).

BBa_K631039 - nahD

The coding sequence for the nahD protein, from pseudomonas peutida, capable of degrading napthalene. Expressing this in our worm may give it the ability to degrade naphthalene.

Regulatory Constructs for C. elegans

BBa_K631035 - Str2

A promoter for AWC neurons, normally used for a 7-transmembrane olfactory receptor. We can use this as a promoter to integrate non-native GPCRs into the AWC neuron membrane.

BBa_K631001 - ODR-7

A promoter for a 7-transmembrane olfactory receptor in AWA olfactory neurons. This can be used for integrating non-native GPCRs. Length: 1503

BBa_K631002 - let-858

A promoter for a protein essential for the development of the worm. We used this in one of our composite parts to express fluorescence in the worm.

BBa_K631003 - GPC-1

A promoter for one of the gamma sub-units of the GPCR binding proteins. We used this for our fluorescence as well. Length: 2000

BBa_K631004 - GCY7

A promoter for a membrane guanylyl cyclase protein. This can be used for a non-native receptor or fluorescence reporter.

Composite Constructs

BBa_K631031 - ODR7:OX1R:UNC-54

A composite part designed to place the non-native OX1R into the neurons of the worm using the ODR-7 promoter for the ability to detect cholorobenzoic acid.

BBa_K631032 - ODR7:ADBR2:UNC-54

A composite part designed to place the non-native ADBR2 into the neurons of the worm using the ODR-7 promoter for the ability to detect phenol (albuteroll). Length: 3666

BBa_K631033 - GCY7:ADBR2:UNC-54

Another composite part with ADBR2, under the GCY7 promoter.

BBa_K631037 - GPC-1:eCFP:UNC-54

This is a composite part for our reporter system using cyano-fluorescent protein under the GPC-1 promoter. Length: 3787

BBa_K631034 - let858:RFP:UNC-54

This is a composite part for our reporter system using red fluorescent protein under the let858 promoter.

BBa_K631038 - GCY-7:eCFP:UNC-54

This is a composite part for our reporter system using cyano-fluorescent protein under the GCY-7 promoter.

BBa_K631036 - eCFP:UNC-54

Eukaryotic cyano-fluorescent protein for the reporter system in our worm. Length: 1629.

Complete List of parts

<groupparts>iGEM011 Queens_Canada</groupparts>

Retrieved from "http://2011.igem.org/Team:Queens_Canada/Parts/Registry"