Team:Grenoble/Projet/Intro

From 2011.igem.org

(Difference between revisions)
(Created page with "{{:Team:Grenoble/Templates/Team:Grenoble_Rawhide}} <html> <div class="left"> <h2> Grenoble Project</h2> <div class="blocbackground"> <img src="https://static.igem.org/mediawiki/2011/...")
 
(81 intermediate revisions not shown)
Line 1: Line 1:
 +
{{:Team:Grenoble/Templates/Current}}
 +
 +
<!--
{{:Team:Grenoble/Templates/Team:Grenoble_Rawhide}}
{{:Team:Grenoble/Templates/Team:Grenoble_Rawhide}}
 +
-->
<html>
<html>
-
<div class="left">
 
-
<h2> Grenoble Project</h2>
 
-
<div class="blocbackground">
 
-
<img src="https://static.igem.org/mediawiki/2011/3/38/Jb.png" class="icon"/>
 
-
<h2> Abstract<span>JB</span></h2>
 
-
<p>
+
<!--
-
Only ranges of pollutant concentration have been measured in the iGEM competition until now. Our goal is to create a simple and accurate, ready-to-use bioquantifier for heavy metals. Simple enough for an easy visual checking and a routine use in the labs. Our system is inspired from Gardner's work on toggle switch biosystem and from former iGem teams' works on quorum sensing communication.</p>
+
<img src="http://www.clickartists.org/clicksite/html/images/square.jpg"/>
-
<p>
+
 
-
Our purpose is to add a pollutant to a device containing a bacterial layer on an engineered medium. This engineered medium is an IPTG gradient.Add of that pollutant to our system induces a geographical division of the plate into two areas : one where the IPTG concentration prevails, and the other where the pollutant concentration prevails. Toggle switch is a biological network which allows to lock our biosystem in a specific state. We use it here in association with complementary quorum sensing genes : A sender and a receiver. Thus, at the interface between both, the reception of quorum sensing molecules by the receptors will induce the coloration of the bacteria. Finally we will obtain a colored line from which we can get the unknown concentration of pollutant.</p>
+
Si vous éditez la page commencez par décommenter ces lignes, publier, PUIS commencer à faire ce que vous avez à faire et quand vous avez fini de publier remettez en commentaire.
-
<p>
+
 
-
Up to now the quantification of heavy metals requires complex physical and chemical protocols. We propose a new way of quantifying heavy metals, much easier than these ones. A first step to a pure water for everyone!</p>
+
Ne laissez pas le carré trop longtemps si vous n'éditez pas, chaque fois reprenez ce qui est sur internet plutôt que ce que vous avez sur votre PC
-
</div>
+
-->
 +
 
 +
 
 +
<div class="left">
 +
 +
<div class="body">
 +
 +
 +
 +
<div class="blocbackground">
 +
 +
<h1>The Project, Mercuro-Coli</h1>
 +
 +
<h2>Introduction</h2>
 +
 +
<p>
 +
With industrial growth, wastes are accumulating and the presence of pollutant and toxic compounds is becoming an international concern.
 +
</p>
 +
<p>
 +
In this context, we present Mercuro-Coli, an easy-to-use biosensor for the in situ detection and quantification of mercury into polluted water. Intended to fieldwork applications, the device should be very easy to handle:
 +
</p>
 +
 +
<center>
 +
<a href="https://static.igem.org/mediawiki/2011/5/58/Dispositif.png"><img src="https://static.igem.org/mediawiki/2011/5/58/Dispositif.png" width=600px/></a>
 +
</center>
 +
<div class="legend">
 +
Easy to use in three steps!
 +
</div>
 +
 +
<ul>
 +
<li>
 +
Unpack the plate.
 +
</li>
 +
<li>
 +
Deposit the polluted water sample.
 +
</li>
 +
<li>
 +
If mercury is present, a red stripe appear, its position indicates the amount of pollutant.
 +
</li>
 +
</ul>
 +
 +
</div>
 +
 +
<div class="blocbackground">
 +
 +
<h2>Specifications</h2>
 +
 +
<p>
 +
At the beginning of the project, we defined a certain number of specifications for the device:
 +
</p>
 +
 +
<ul>
 +
<li>
 +
<strong>Use a single bacterial strain: <i>Escherichia coli</i> BW 25 113, containing the whole genetic network</strong>
 +
</li>
 +
<li>
 +
<strong>Form a biofilm on the plate with <i>E. coli</i> bacteria containing the designed genetic circuit.</strong><br/>
 +
This point was revised according to <a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Quorum#Simulation" title="Optimization of the device thanks to modelling">modeling results.</a> A device with channels containing bacteria instead of a biofilm has been chosen.  
 +
</li>
 +
</ul>
 +
 +
<center>
 +
<a href="https://static.igem.org/mediawiki/2011/c/c5/1_type_bacteria.png"><img align="middle" src="https://static.igem.org/mediawiki/2011/c/c5/1_type_bacteria.png" width=600px/></a>
 +
</center>
 +
<div class="legend">
 +
Plate covered by the bacteria.
 +
</div>
 +
 +
<ul>
 +
<li><strong>Build a comparative measurement system:</strong></li>
 +
</ul>
 +
 +
<table class="nobordure">
 +
<tr>
 +
<td><a href="https://static.igem.org/mediawiki/2011/0/07/Plaque_mer_iptg.png"><img src="https://static.igem.org/mediawiki/2011/0/07/Plaque_mer_iptg.png"  height="350px"></a></td>
 +
<td>
 +
<p>
 +
The principle of the measurement is based on the comparison between an unknown mercury concentration and a known IPTG concentration. In practice, the quantification scale is made by the prior application of a concentration gradient of IPTG on the plate.
 +
</p>
 +
</td>
 +
</tr>
 +
</table>
 +
 +
<p>
 +
Two different bacterial behaviors arise from the uniform addition of polluted sample on the plate. They depend on the predominant concentration:
 +
</p>
 +
 +
<table class="nobordure">
 +
<tr>
 +
<td>
 +
<ul>
 +
<li>
 +
On the left side, where the mercury concentration is prevailing, bacteria will behave as <strong>senders</strong>. They have the ability to release quorum sensing molecules (AHL) in the external medium, thanks to the expression of the CinI protein.
 +
</li>
 +
<li>
 +
On the right, where the IPTG concentration is dominant, bacteria will behave as <strong>receivers</strong>. They express CinR protein, a cytoplasmic receptor for quorum sensing.
 +
</li>
 +
</ul>
 +
</td>
 +
<td>
 +
<center>
 +
<a href="https://2011.igem.org/Team:Grenoble/Projet/Design/quorum" title="Click for more details on the relation between the two bahaviours and the quorum sensing"><img src="https://static.igem.org/mediawiki/2011/0/00/Receiving_secr_bacteria.png"  width="450px"></a>
 +
</center>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p>
 +
In summary, two areas with distinctive bacterial behavior appear on the plate. They are separated by one boundary that approaches one side of the plate or the other, depending on the mercury level. For example, in the case of a sample with a low mercury concentration, the boundary will appear closer to the left side.
 +
</p>
 +
</td>
 +
<td>
 +
<a href="https://static.igem.org/mediawiki/2011/d/d8/Border_movement.png"><img src="https://static.igem.org/mediawiki/2011/d/d8/Border_movement.png"  width="450px"></a>
 +
</td>
 +
</tr>
 +
</table>
 +
 +
<p>
 +
The key point is therefore the localization of the boundary, which we need to visualize
 +
</p>
 +
 +
<ul>
 +
<li><strong>A visual result:</strong></li>
 +
</ul>
 +
 +
<center>
 +
<table class="nobordure">
 +
<tr>
 +
<td>
 +
<a href="https://2011.igem.org/Team:Grenoble/Projet/Design/quorum#coloration" title="click for more details on the appearance of the red stripe"><img src="https://static.igem.org/mediawiki/2011/0/02/Coloration.png"  width="400px"></a>
 +
</td>
 +
<td>
 +
<a href="https://2011.igem.org/Team:Grenoble/Projet/Design/quorum#coloration" title="click for more details on the appearance of the red stripe"><img src="https://static.igem.org/mediawiki/2011/c/c2/Complex.png"  width="340px"></a>
 +
</td>
 +
</tr>
 +
</table>
 +
</center>
 +
 +
<p>
 +
At the boundary,Quorum Sensing molecule, AHL, released by sending bacteria will be uptaken by the front of receiving bacteria. The complex formed by AHL molecules and CinR proteins will then induce the coloration of the front receiving bacteria.
 +
</p>
 +
 +
<center>
 +
<a href="https://2011.igem.org/Team:Grenoble/Projet/Design" title="Click here"><img src="https://static.igem.org/mediawiki/2011/1/1b/Bouton_general_biologie.png"/></a>
 +
<div class="legend">
 +
Let's go further to understand how such evolution of this system is possible with only one strain ?
 +
</div>
 +
</center>
 +
</div>
 +
 +
</div>
 +
 +
</div>
 +
 +
<script>
 +
document.getElementById('submenu').innerHTML = '<h3><span class="vert">Le Projet:</span> Bio</h3><ul><li><a href="https://2011.igem.org/Team:Grenoble/Projet/Intro">Introduction</a></li><li><a href="https://2011.igem.org/Team:Grenoble/Projet/Device">The device</a></li><li><a href="https://2011.igem.org/Team:Grenoble/Projet/Design">The genetic circuit:</a></li><ol><li><a href="https://2011.igem.org/Team:Grenoble/Projet/Design/toggle">The toggle switch</a></li><li><a href="https://2011.igem.org/Team:Grenoble/Projet/Design/quorum">The quorum sensing</a></li><li><a href="https://2011.igem.org/Team:Grenoble/Projet/Design/quorum#color">The coloration</a></li><li><a href="https://2011.igem.org/Team:Grenoble/Projet/regulation">Post-transcriptional regulation</a></li></ol><li><a href="https://2011.igem.org/Team:Grenoble/Projet/Modelling">Modelling</a></li><li><a href="https://2011.igem.org/Team:Grenoble/Projet/Results">Results</a></li><li><a href="https://2011.igem.org/Team:Grenoble/Projet/Biobricks">Biobricks</a></li></ul>'
 +
</script>
 +
 
</html>
</html>
 +
 +
{{:Team:Grenoble/Design/pied}}

Latest revision as of 23:03, 28 October 2011

Grenoble 2011, Mercuro-Coli iGEM


The Project, Mercuro-Coli

Introduction

With industrial growth, wastes are accumulating and the presence of pollutant and toxic compounds is becoming an international concern.

In this context, we present Mercuro-Coli, an easy-to-use biosensor for the in situ detection and quantification of mercury into polluted water. Intended to fieldwork applications, the device should be very easy to handle:

Easy to use in three steps!
  • Unpack the plate.
  • Deposit the polluted water sample.
  • If mercury is present, a red stripe appear, its position indicates the amount of pollutant.

Specifications

At the beginning of the project, we defined a certain number of specifications for the device:

  • Use a single bacterial strain: Escherichia coli BW 25 113, containing the whole genetic network
  • Form a biofilm on the plate with E. coli bacteria containing the designed genetic circuit.
    This point was revised according to modeling results. A device with channels containing bacteria instead of a biofilm has been chosen.
Plate covered by the bacteria.
  • Build a comparative measurement system:

The principle of the measurement is based on the comparison between an unknown mercury concentration and a known IPTG concentration. In practice, the quantification scale is made by the prior application of a concentration gradient of IPTG on the plate.

Two different bacterial behaviors arise from the uniform addition of polluted sample on the plate. They depend on the predominant concentration:

  • On the left side, where the mercury concentration is prevailing, bacteria will behave as senders. They have the ability to release quorum sensing molecules (AHL) in the external medium, thanks to the expression of the CinI protein.
  • On the right, where the IPTG concentration is dominant, bacteria will behave as receivers. They express CinR protein, a cytoplasmic receptor for quorum sensing.

In summary, two areas with distinctive bacterial behavior appear on the plate. They are separated by one boundary that approaches one side of the plate or the other, depending on the mercury level. For example, in the case of a sample with a low mercury concentration, the boundary will appear closer to the left side.

The key point is therefore the localization of the boundary, which we need to visualize

  • A visual result:

At the boundary,Quorum Sensing molecule, AHL, released by sending bacteria will be uptaken by the front of receiving bacteria. The complex formed by AHL molecules and CinR proteins will then induce the coloration of the front receiving bacteria.

Let's go further to understand how such evolution of this system is possible with only one strain ?

logo iGEM
iGEM 2011 Main Page


Contact Us:

Clic here !

Retrieved from "http://2011.igem.org/Team:Grenoble/Projet/Intro"