From 2011.igem.org

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Latest revision as of 18:41, 28 October 2011

Notebook - September

Lab Notes

July August
September
October

Protocol

Brainstorm

Lab Notes - October

Biobrick Group

Week13

Day

Note

Sept.26th Monday

•Miniprep: Rush-3,-4,-12
•Run the gel of digestion test results:
RFP+YFP+1K3-7,-16

•PCR amplify: Rush-3,-4,-12
•Sample and test the intensity of fluorescence once or twice an hour during the growth of E.coli

Sept.27th Tuesday

•Ligate and transform:
RFP+YFP+CFP+1C3 RFP+YFP+1C3

•PCR amplify:20H
•Cut: Rush Bone,22B

Sept.28th Wednesday

•Cultivate: DH5αwith Device CFP,RFP,YFP and without any device
•Ligate and purify:Rush+22B
•Cut and purify:
the product of the ligation

•Run the gel: Digested Rush and 22B,Lig1
•Self-ligate:Digested Lig
•Colony PCR: RFP+YFP+CFP+1C3
•Cut and purify: RFP+YFP+1K3,12I

Sept.29th Thursday

•Ligate and transform: RFP+YFP+1K3&1C3,
RFP+YFP&1C3
•Colony PCR:RFP+YFP+CFP+1C3
•Preserve the colony on the plate

•Plate the ligation results: RFP+1C3,
RFP+YFP+CFP+1C3
Colony PCR:RFP+YFP+1C3
RFP+YFP+CFP+1C3
•Cultivate the above

Sept.30th Friday

•Colony PCR: Rush-Lig

Oct.1st Saturday

•Colony PCR: Failed
•MIniprep: 10I

•Cut: 10I+22B,20J,1T3
•Ligate:10I+22B+1T3

•Cultivate:2I,2M

Oct.2nd Sunday

•Transform the ligation results:
22B+10I(1T3),20J+10I(1T3)
•Miniprep and Preserve:2M,2I

•Cut:fdhf,Vgb,2M,2I
•Purify:RFP+YFP+CFP+1C3

•Ligate and transform:
RFP+YFP+CFP&1A3k
fdhf+2M&1K3,Vgb+2I&1K3

Week14

Day

Note

Oct.3rd Monday

•Colony PCR

Oct.4th Tuesday

•Cut:RBS+20J
•Cultivate:fdhf+2M-1,-2,-3,-4
Vgb+2I-1,-222B+10I-1,-2
•Ligate and purify:RBS+20J& 1C3

•Cut:RBS+20J
•Ligate:2M+20J&10I to 1K3
•Transform:2M+20J+10I,Vgb+1C3
•Cultivate:22B+10I-1,-2

•Miniprep and cut: fdhf+2M,Vgb+2I
•Cut:fdhf+2M,20H

Oct.5th Wednesday

•Cultivate:Vgb+2I 1-6
•Ligate:fdhf+20H& 1K3

•Transform:fdhf+20H
•Cut:2M+20J

Oct.6th Thurday

Finishing the wiki

Oct.7th Friday

making poster

Oct.8th Saturday

•Miniprep and test:
Vgb,fdhf+20H+1K3 1-4

•Colony PCR

Oct.9th Sunday

•Digestion test and run the gel to test the results:
20J+10I -1,-2,-4
22B+10I -1,-2,-3,-4 Vgb+1C3
Fdhf+2M+20H -1,-2,-3,-4 Vgb+2I -1,-2,-5

•Colony PCR:fdhf+2M+20H 5-14
•Cultivate:fdhf+2M+20H 5-14
•Cut:fdhf+2M,20H

Week15

Day

Note

Oct.10th Monday

•Ligate:Fdhf+2M&20H to 1C3
•Cultivate:16L+Device C

•Cut:Vgb+2I,22B+10I
•Ligate:Vgb+2I&22B+10I to1C3

Oct.11th Tuesday

•Cultivate:20H,22B+10I-1,20J+10I-1,-4

•Miniprep and cut:
fdhf+2M+20H+1K3 5-14

Oct.12th Wednesday

•Miniprep: fdhf+20H-10,20H,22B+10I, 20J+10I-1,-4 fdhf+2I+20H,

•Cut and run the gel: Vgb+2I-5,
22B+10I,20J+10I,1C3 backbone

Oct.13th Thursday

•Cut and run the gel to test the ligate results

Oct.14th Friday

•Regional Jamboree @ HKUST

Oct.15th Saturday

•Regional Jamboree @ HKUST

Oct.16th Sunday

•Regional Jamboree @ HKUST

We are the Champion!!!

Week16

Day

Note

Oct.17th Monday

•Rest

Oct.18th Tuesday

•Rest

Oct.19th Wednesday

•Transform: Kit Plate 1 18E&18I, Kit Plate 4 7K& 7M

Oct.20th Thursday

•Transform: Kit Plate 4 7K
•Colony PCR and run the gel:23L 1-6

Oct.21st Friday

•Transform:7K
•Cultivate:7M,18I,18E,23L pSB1A3,1K3,1C3 backbone

•Cut and test the resistance: 22M+10I
•Colony PCR:7M 1-7,18E 1-6,18I 1-6

Oct. 22nd Saturday

•Cultivate:pSB1C3-5,23L-1,-2,7M-1
•Cultivate:7K,10I,22M,18E,18I
•Colony PCR:18E 1-6,18I 1-6
•Test the reagent for PCR several times

•Miniprep and digestion test: 7M,23L,1K3,1A3,1C3
•Digestion and run the gel: 7M,22M+10I-2,1K3

Oct.23rd Sunday

•Cut:7M,22M+10I
•Digestion test:18I,18E

•Ligate and transform: 7M+22M+10I& 1K3

Week17

Day

Note

Oct.24th Monday

•Transform: Kit Plate 4 8G,8I Kit Plate 1 1F
•Culture yeast & B. subtilis

•Colony PCR and run the gel: 7M+22M+10I+1K3 1-25
•Cultivate:8G,1F,8I

Oct.25th Tuesday

•Miniprep:7M+22M+10I+1K3
•Colony PCR:8I,8G,1F
•Cut:7M+22M+10I+1K3,1C3 backbone

•Cultivate:7K
•Ligate:7M+22M+10I& 1C3
•Genome DNA extraction from yeast & B. subtilis
•PCR condition pre-test for alsS & Adh2

Oct.26th Wednesday

•Cultivate:8G,8I,1F,7M,18E,18I
•Transform:7M+22M+10I+1C3 7M+22M+10I+1K3-2
•Reserve:7M+22M+10I+1K3,8G,8I, 1F,7M,18E,18I

•Miniprep:8G
•Cultivate:7M+22M+10I+1C3，2J
•Testify PCR results on gel
•Clean-up alsS PCR product
•Re-pre-test PCR condition for Adh2

Oct.27th Thursday

•PCR producing Adh2
•alsS ES double-digestion
•purification both

Oct.28th Friday

Oct. 29th Saturday

Oct.30th Sunday

Biofilm Group

3rd Oct

18 Oct

Biofilm formation of AI2+rainbofilm device without induction of AI2 for 48 hours.

19 Oct

Co-transformation of AHL and rainbofilm device.

20 Oct

Observation of rainbofilm. Maximum thickness is 14 micrometer.

22 Oct

Biofilm formation with or without AI2 (by arabinose induction of part BBa_K091191) for 48, 36, 24 hours.

24 Oct

Laser confocal observation of biofilm. Used too much water to wash away salt crystals and although thick biofilm is observed before washing but very thin biofilm is observed under laser confocal.

25 Oct

Biofilm formation with rainbofilm DH5alpha without quorum sensing for 48 hours. Colony PCR of 20 JM109 AI2-rainbofilm co-transformaion. Non has rainbofilm plasmid successfully transformed.

26 Oct

18 AI2-rainbofilm JM109 and 20 AHL-rainbofilm DH5alpha colony PCR. Number 5,6,14 of AI2 and number 5,8,11 of AHL have both plasmids.

27 Oct

Biofilm formation with AI2, AHL induced in rainbofilm DH5alpha AHL, DH5alpha AI2 and JM109 AI2.

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-				<td>•Transform: Kit Plate 4 8G,8I Kit Plate 1 1F </td>
+				<td>•Transform: Kit Plate 4 8G,8I Kit Plate 1 1F <br/>
+               •Culture yeast & B. subtilis</td>
                  <td>•Colony PCR and run the gel: 7M+22M+10I+1K3 1-25<br/>•Cultivate:8G,1F,8I</td>
 			</tr>
@@ Line 579: / Line 578: @@
 			<tr>
 				<td>•Miniprep:7M+22M+10I+1K3<br/>•Colony PCR:8I,8G,1F<br/>•Cut:7M+22M+10I+1K3,1C3 backbone</td>
-                 <td>•Cultivate:7K<br/>•Ligate:7M+22M+10I& 1C3</td>
+                 <td>•Cultivate:7K<br/>•Ligate:7M+22M+10I& 1C3<br/> •Genome DNA extraction from yeast & B. subtilis<br/> •PCR condition pre-test for alsS & Adh2</td>
 			</tr>
 		</table>
@@ Line 590: / Line 589: @@
 			<tr>
 				<td>•Cultivate:8G,8I,1F,7M,18E,18I<br/>•Transform:7M+22M+10I+1C3 7M+22M+10I+1K3-2<br/>•Reserve:7M+22M+10I+1K3,8G,8I, 1F,7M,18E,18I</td>
-                 <td>•Miniprep:8G<br/>•Cultivate:7M+22M+10I+1C3，2J</td>
+                 <td>•Miniprep:8G<br/>•Cultivate:7M+22M+10I+1C3，2J<br/>•Testify PCR results on gel<br/>•Clean-up alsS PCR product<br/>•Re-pre-test PCR condition for Adh2</td>
 			</tr>
 		</table>
@@ Line 599: / Line 598: @@
 		<td><table width="611" border="0" cellspacing="0" cellpadding="1">
 		  <tr>
-		    <td ></td>
+		    <td > •PCR producing Adh2<br/>•alsS ES double-digestion<br/>•purification both</td>
 		    </tr>
 		  </table></td>
@@ Line 656: / Line 655: @@
 </div>
 <div class="block" id="nsheet">
-<h1>4th Oct</h1>
+<h1>18 Oct</h1>
-<p></p>
+<p>Biofilm formation of AI2+rainbofilm device without induction of AI2 for 48 hours.</p>
 </div>
 <div class="block" id="nsheet">
-<h1>6th Oct</h1>
+<h1>19 Oct</h1>
-<p></p>
+<p>Co-transformation of AHL and rainbofilm device.</p>
 </div>
 <div class="block" id="nsheet">
-<h1>12th Oct</h1>
+<h1>20 Oct</h1>
-<p></p>
+<p>Observation of rainbofilm. Maximum thickness is 14 micrometer.</p>
 </div>
 <div class="block" id="nsheet">
-<h1>15th Oct</h1>
+<h1>22 Oct</h1>
-<p></p>
+<p>Biofilm formation with or without AI2 (by arabinose induction of part BBa_K091191)
+for 48, 36, 24 hours.</p>
 </div>
 <div class="block" id="nsheet">
-<h1>16th Oct</h1>
+<h1>24 Oct</h1>
-<p></p>
+<p>Laser confocal observation of biofilm. Used too much water to wash away salt
+crystals and although thick biofilm is observed before washing but very thin biofilm is
+observed under laser confocal.</p>
 </div>
 <div class="block" id="nsheet">
-<h1>17th Oct</h1>
+<h1>25 Oct</h1>
-<p></p>
+<p>Biofilm formation with rainbofilm DH5alpha without quorum sensing for 48 hours.
+Colony PCR of 20 JM109 AI2-rainbofilm co-transformaion. Non has rainbofilm plasmid
+successfully transformed.</p>
 </div>
 <div class="block" id="nsheet">
-<h1>20th Oct</h1>
+<h1>26 Oct</h1>
-<p></p>
+<p>18 AI2-rainbofilm JM109 and 20 AHL-rainbofilm DH5alpha colony PCR. Number
+,6,14 of AI2 and number 5,8,11 of AHL have both plasmids.</p>
+</div>
+<div class="block" id="nsheet">
+<h1>27 Oct</h1>
+<p>Biofilm formation with AI2, AHL induced in rainbofilm DH5alpha AHL, DH5alpha
+AI2 and JM109 AI2.</p>
 </div>
 </div>
 <script type="text/javascript">

Team:ZJU-China/October.html

From 2011.igem.org

Latest revision as of 18:41, 28 October 2011

Lab Notes

Protocol

Brainstorm

Lab Notes - October

Biobrick Group

Week13

Week14

Week15

Week16

Week17

Biofilm Group

3rd Oct

18 Oct

19 Oct

20 Oct

22 Oct

24 Oct

25 Oct

26 Oct

27 Oct