Team:HKUST-Hong Kong/data.html
From 2011.igem.org
(Difference between revisions)
(14 intermediate revisions not shown) | |||
Line 8: | Line 8: | ||
</style> | </style> | ||
- | |||
- | |||
<style type="text/css"> | <style type="text/css"> | ||
<!-- | <!-- | ||
- | A:link { COLOR: black | + | A:link { COLOR: black; font-weight: normal } |
- | A:visited { COLOR: black; | + | A:visited { COLOR: black; font-weight: normal } |
+ | |||
+ | A:active { COLOR: black;} | ||
+ | |||
+ | A:hover { COLOR: black; font-weight: normal ; text-decoration:underline} | ||
- | |||
- | |||
--> | --> | ||
p{ | p{ | ||
- | margin: 20px 20px | + | font-family: Georgia, Helvetica, Arial, New Times Roman; |
+ | font-size:14; | ||
+ | line-height: 18pt; | ||
+ | font-weight:normal; | ||
+ | margin: 0px 20px 0px 20px; | ||
+ | text-align: justify; | ||
} | } | ||
+ | |||
table{ | table{ | ||
margin: 0px 0px 0px 0px | margin: 0px 0px 0px 0px | ||
} | } | ||
</style> | </style> | ||
- | |||
- | |||
- | |||
</head> | </head> | ||
- | |||
- | |||
- | |||
<table style="border-collapse: collapse" width=963> | <table style="border-collapse: collapse" width=963> | ||
<TR bgcolor="#9CC3B1"><td> | <TR bgcolor="#9CC3B1"><td> | ||
- | + | ||
<p align="center"><font face="Verdana, Arial, Helvetica, sans-serif" size="7" color="white"> | <p align="center"><font face="Verdana, Arial, Helvetica, sans-serif" size="7" color="white"> | ||
- | <br>Data Page< | + | <br>Data Page</font></p> |
+ | |||
+ | |||
+ | <br> | ||
Line 53: | Line 56: | ||
<TR bgcolor="white"><td> | <TR bgcolor="white"><td> | ||
- | <center><img src=https://static.igem.org/mediawiki/2011/ | + | <center> |
+ | <a href=https://static.igem.org/mediawiki/2011/6/64/Ust_Merge.jpg> | ||
+ | <img src=https://static.igem.org/mediawiki/2011/6/64/Ust_Merge.jpg width=950 height=1096> | ||
+ | </a> | ||
+ | <br> | ||
+ | <font size=2 color=gray>Click to enlarge</font> | ||
+ | </center> | ||
Line 59: | Line 68: | ||
<blockquote> | <blockquote> | ||
- | 1. <a href=http://partsregistry.org/Part:BBa_K524000><font color=blue>BBa_K524000</font></a> | + | 1. <a href=http://partsregistry.org/Part:BBa_K524000><font color=blue>BBa_K524000</font></a> – oriR101 & repA101-ts: This heat- sensitive origin of replication has been shown to be functional at 30°C. Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.<br><br> |
</blockquote> | </blockquote> | ||
Line 65: | Line 74: | ||
<blockquote> | <blockquote> | ||
- | 1. <a href=http://partsregistry.org/Part:BBa_K524001><font color=blue>BBa_K524001</font></a> | + | 1. <a href=http://partsregistry.org/Part:BBa_K524001><font color=blue>BBa_K524001</font></a> – pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.<br><br> |
- | 2. <a href=http://partsregistry.org/Part:BBa_K524002><font color=blue>BBa_K524002</font></a> | + | 2. <a href=http://partsregistry.org/Part:BBa_K524002><font color=blue>BBa_K524002</font></a> – pLac + RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.<br><br> |
- | 3. <a href=http://partsregistry.org/Part:BBa_K524003><font color=blue>BBa_K524003</font></a> – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD+ | + | 3. <a href=http://partsregistry.org/Part:BBa_K524003><font color=blue>BBa_K524003</font></a> – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD<sup>+</sup> synthetase.<br><br> |
- | 4. <a href=http://partsregistry.org/Part:BBa_K524004><font color=blue>BBa_K524004</font></a> | + | 4. <a href=http://partsregistry.org/Part:BBa_K524004><font color=blue>BBa_K524004</font></a> – pir gene: the pir gene encodes the autogenously regulated pi protein.<br><br> |
5. <a href=http://partsregistry.org/Part:BBa_K524005><font color=blue>BBa_K524005</font></a> – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.<br><br> | 5. <a href=http://partsregistry.org/Part:BBa_K524005><font color=blue>BBa_K524005</font></a> – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.<br><br> | ||
- | 6. <a href=http://partsregistry.org/Part:BBa_K524006><font color=blue>BBa_K524006</font></a> | + | 6. <a href=http://partsregistry.org/Part:BBa_K524006><font color=blue>BBa_K524006</font></a> – RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.<br><br> |
- | 7. <a href=http://partsregistry.org/Part:BBa_K524007><font color=blue>BBa_K524007</font></a> | + | 7. <a href=http://partsregistry.org/Part:BBa_K524007><font color=blue>BBa_K524007</font></a> – RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.<br><br> |
8. <a href=http://partsregistry.org/Part:BBa_K524100><font color=blue>BBa_K524100</font></a> – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.<br><br> | 8. <a href=http://partsregistry.org/Part:BBa_K524100><font color=blue>BBa_K524100</font></a> – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.<br><br> | ||
Line 87: | Line 96: | ||
</font> | </font> | ||
- | |||
- | </ | + | |
- | + | </TH> | |
- | < | + | |
+ | |||
+ | |||
+ | </TD> | ||
+ | </TR> | ||
+ | |||
+ | </table> | ||
+ | |||
Line 106: | Line 121: | ||
<tr> | <tr> | ||
<td width="100px" height="150px"; bgcolor="#980000" > | <td width="100px" height="150px"; bgcolor="#980000" > | ||
- | <p align="center | + | <p align="center"> |
- | <a href=https://2011.igem.org/Team:HKUST-Hong_Kong target=_top><font | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong" target=_top> |
+ | <b><font color="#FFE1E1" size=3>Home</font></b> | ||
</p> | </p> | ||
</td> | </td> | ||
- | <td width=" | + | <td width="382px" bgcolor="#CCFF99" valign="baseline"> |
- | <p align=" | + | <p align="center" valign="baseline"> |
+ | <b><font color="green">Our Project</font></b></p> | ||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a><font color="green"> | </font> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br></p> | ||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="green">Experiments and Results</font></b></p> | ||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/asm.html" target=_top>Strain Construction</a><font color="green"> | </font> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/mic.html" target=_top>Culture Tests</a><font color="green"> | </font> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/modeling.html" target=_top>Modeling</a><br></p> | ||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="green">Miscellaneous</font></b></p> | ||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/notebook.html" target=_top>Notebook</a></p> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
</td> | </td> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
+ | <td width="302px" bgcolor="#D09C00" valign="baseline"> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="#FFF4D0">iGEM Resources</font></b></p> | ||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a></p> | ||
+ | |||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="#FFF4D0">The Team</font></b></p> | ||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/team.html" target=_top>iGEM Member List</a><font color="#FFF4D0"> | </font> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/contribution.html" target=_top>Contributions</a><br></p> | ||
+ | |||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="#FFF4D0">Achievements</font></b></p> | ||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements<font color="#FFF4D0"> | </font> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br></p> | ||
+ | |||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="#FFF4D0">BioBricks</font></b></p> | ||
+ | |||
+ | |||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/characterization.html" target=_top>Master List & Characterization Data</a><br></p> | ||
- | |||
- | |||
</td> | </td> | ||
- | <td width=" | + | <td width="180px"bgcolor="#980000"valign="baseline"> |
- | <p align=" | + | <p align="center" valign="baseline"> |
- | <b> | + | <b><font color="#FFE0E0">Human Practice</font></b></p> |
- | <font | + | |
- | </font> | + | <p align="center" valign="baseline"> |
- | </b> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop</a><font color="white"> | </font> |
- | </p> | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a><br></p> |
- | <p align=" | + | |
- | <a href="workshop.html" target=_top>Workshop</a> | + | |
- | <font | + | |
- | <a href="survey.html" target=_top>Survey</a> | + | |
- | |||
</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
- | |||
- | |||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 18:22, 28 October 2011
|
![]() Click to enlarge
1. BBa_K524000 – oriR101 & repA101-ts: This heat- sensitive origin of replication has been shown to be functional at 30°C. Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.
1. BBa_K524001 – pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010. |
Our Project Experiments and Results
Strain Construction |
Culture Tests |
Modeling Miscellaneous |
iGEM Resources The Team
iGEM Member List |
Contributions Achievements
Medal Requirements |
BioSafety BioBricks |
Human Practice |