Team:uOttawa

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Yeast has become a model organism due to its homology with mammalian organisms and its ease of genetic manipulation. For this reason, we believe it is important to develop tools that improve our ability to use yeast as a standard BioBrick organism. To this end, we aim to submit several BioBricks optimized to work in the simple eukaryote S. cerevisiae as well as build a novel yeast chassis that is capable of the robust characterization of these Biobricks. In addition, we are developing a new assembly standard that will greatly improve upon the existing standards for BioBrick assembly and allow for rapid integration into S. cerevisiae.
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!align="center"|[[Team:uOttawa|Home]]
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!align="center"|[[Team:uOttawa/Team|Team]]
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<div class="main_view" style="margin-bottom:15px; margin-top:20px;">
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=uOttawa Official Team Profile]
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!align="center"|[[Team:uOttawa/Project|Project]]
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!align="center"|[[Team:uOttawa/Parts|Parts Submitted to the Registry]]
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            <a href="/Team:uOttawa/Team"><img border="0" src="https://static.igem.org/mediawiki/2011/9/94/Uo_team.png" alt="Sponsors" /></a>
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!align="center"|[[Team:uOttawa/Modeling|Modeling]]
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!align="center"|[[Team:uOttawa/Notebook|Notebook]]
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!align="center"|[[Team:uOttawa/Safety|Safety]]
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            <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=uOttawa" target="_blank"><img border="0" src="https://static.igem.org/mediawiki/2011/f/f4/Uo_parts-submitted.png" alt="Sponsors" /></a>
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!align="center"|[[Team:uOttawa/Attributions|Attributions]]
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            <a href="/Team:uOttawa/Game"><img border="0" src="https://static.igem.org/mediawiki/2011/0/0c/Uo_gears-of-evolution.png" alt="Sponsors" /></a>
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            <a href="/Team:uOttawa/Sponsors"><img border="0" src="https://static.igem.org/mediawiki/2011/7/7d/Uo_sponsors.png" alt="Sponsors" /></a>
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<p> <i>Saccharomyces cerevisiae</i> (budding or brewer's yeast) has emerged as an important micro-organism in biomedical research and industry. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in <i>S. cerevisiae</i>. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have also submitted several BioBricks optimized for use in <i>S. cerevisiae</i>. Finally, we succeeded in developing BrickMason Assembly, a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols. So raise a glass to your favourite single-celled eukaryote, and let yeast leaven your spirits.</p>
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<html><a href="/Team:uOttawa/Team"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/8/88/Team_uo.png') no-repeat !important;"><span>Team</span></h2></a></html><p>This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.</p>
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<html><a href="/Team:uOttawa/NewAssemblyProtocol"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/c/cd/BM3.png') no-repeat !important;"><span>BrickMason Assembly</span></h2></a></html><p>Here we describe a convenient, efficient and cost-effective assembly method known as BrickMason assembly. Click on the image above to check out our animation and see how it works!</p>
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<html><a href="/Team:uOttawa/Results"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/5/51/Results_uo.png') no-repeat !important;"><span>Results</span></h2></a></html><p>Here we successfully demonstrate that BrickMason assembly can be used to assemble 6 brick constructs in just one days work. In addition, we were able to characterize 3 new BioBricks.</p>
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<html><a target="_blank" href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=uOttawa"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/2/2d/Parts_uo.png') no-repeat !important;"><span>Parts Submitted</span></h2></a></html><p>We submitted 8 new BioBricks to the registry this year.</p>
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<html><a href="/Team:uOttawa/Game"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/0/0d/Human_uo.png') no-repeat !important;"><span>Gears of Evolution</span></h2></a></html><p>The uOttawa team has designed a 10 level game intended to teach and inform high school students about synthetic biology. This game is fully playable on our wiki, the iGEM community page and the Openwetware site Community Bricks. Click on the image above to play it and please give us feedback!</p>
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<html><a href="/Team:uOttawa/Sponsors"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/1/1f/Sponsors_uo.png') no-repeat !important;"><span>Sponsors</span></h2></a></html><p>The uOttawa team would like to thank our sponsors for their generous support this year. Our sponsors graciously supplied us with lab equipment, reagents and funds necessary for the team to compete.</p>
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Latest revision as of 07:52, 27 October 2011


Sponsors Sponsors Sponsors Sponsors Sponsors Sponsors
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Saccharomyces cerevisiae (budding or brewer's yeast) has emerged as an important micro-organism in biomedical research and industry. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in S. cerevisiae. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have also submitted several BioBricks optimized for use in S. cerevisiae. Finally, we succeeded in developing BrickMason Assembly, a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols. So raise a glass to your favourite single-celled eukaryote, and let yeast leaven your spirits.

Team

This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.

BrickMason Assembly

Here we describe a convenient, efficient and cost-effective assembly method known as BrickMason assembly. Click on the image above to check out our animation and see how it works!

Results

Here we successfully demonstrate that BrickMason assembly can be used to assemble 6 brick constructs in just one days work. In addition, we were able to characterize 3 new BioBricks.

Parts Submitted

We submitted 8 new BioBricks to the registry this year.

Gears of Evolution

The uOttawa team has designed a 10 level game intended to teach and inform high school students about synthetic biology. This game is fully playable on our wiki, the iGEM community page and the Openwetware site Community Bricks. Click on the image above to play it and please give us feedback!

Sponsors

The uOttawa team would like to thank our sponsors for their generous support this year. Our sponsors graciously supplied us with lab equipment, reagents and funds necessary for the team to compete.

Retrieved from "http://2011.igem.org/Team:uOttawa"