Team:uOttawa

From 2011.igem.org

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Latest revision as of 07:52, 27 October 2011

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Saccharomyces cerevisiae (budding or brewer's yeast) has emerged as an important micro-organism in biomedical research and industry. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in S. cerevisiae. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have also submitted several BioBricks optimized for use in S. cerevisiae. Finally, we succeeded in developing BrickMason Assembly, a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols. So raise a glass to your favourite single-celled eukaryote, and let yeast leaven your spirits.

Team

This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.

BrickMason Assembly

Here we describe a convenient, efficient and cost-effective assembly method known as BrickMason assembly. Click on the image above to check out our animation and see how it works!

Results

Here we successfully demonstrate that BrickMason assembly can be used to assemble 6 brick constructs in just one days work. In addition, we were able to characterize 3 new BioBricks.

Parts Submitted

We submitted 8 new BioBricks to the registry this year.

Gears of Evolution

The uOttawa team has designed a 10 level game intended to teach and inform high school students about synthetic biology. This game is fully playable on our wiki, the iGEM community page and the Openwetware site Community Bricks. Click on the image above to play it and please give us feedback!

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-<p> <i>Saccharomyces cerevisiae</i> (budding or brewer's yeast) has emerged as an important micro-organism in biomedical research and industry. Not only is this yeast an important model organism due to its homology to higher eukaryotes, but it sees wide commercial application as well. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in <i>S. cerevisiae</i>. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have also submitted several BioBricks optimized for use in <i>S. cerevisiae</i>. Finally, we succeeded in developing a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols, not just for those manipulating yeast but for the whole BioBrick community. So raise a glass to your favourite single-celled eukaryote, and let yeast leaven your spirits.</p>
+<p> <i>Saccharomyces cerevisiae</i> (budding or brewer's yeast) has emerged as an important micro-organism in biomedical research and industry. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in <i>S. cerevisiae</i>. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have also submitted several BioBricks optimized for use in <i>S. cerevisiae</i>. Finally, we succeeded in developing BrickMason Assembly, a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols. So raise a glass to your favourite single-celled eukaryote, and let yeast leaven your spirits.</p>
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-				<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/8/88/Team_uo.png') no-repeat !important;"><span>Team</span></h2></html><p>This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.</p>
+				<html><a href="/Team:uOttawa/Team"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/8/88/Team_uo.png') no-repeat !important;"><span>Team</span></h2></a></html><p>This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.</p>
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-				<html><a href="/Team:uOttawa/NewAssemblyProtocol"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/b/b9/Project_uo.png') no-repeat !important;"><span>BrickMason</span></h2></a></html><p>Characterization and standardizing of biological parts is a continuing problem for synthetic biologists. This year we decided to focus on developing a more efficient assembly method as well as optimizing characterization of BioBricks in S. cerevisiae.</p>
+				<html><a href="/Team:uOttawa/NewAssemblyProtocol"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/c/cd/BM3.png') no-repeat !important;"><span>BrickMason Assembly</span></h2></a></html><p>Here we describe a convenient, efficient and cost-effective assembly method known as BrickMason assembly. Click on the image above to check out our animation and see how it works!</p>
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-				<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/5/51/Results_uo.png') no-repeat !important;"><span>Results</span></h2></html><p>We were able to successfully create a new assembly method as well as characterize a number of transcription factors.</p>
+				<html><a href="/Team:uOttawa/Results"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/5/51/Results_uo.png') no-repeat !important;"><span>Results</span></h2></a></html><p>Here we successfully demonstrate that BrickMason assembly can be used to assemble 6 brick constructs in just one days work. In addition, we were able to characterize 3 new BioBricks.</p>
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-				<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/2/2d/Parts_uo.png') no-repeat !important;"><span>Parts Submitted</span></h2></html><p>We submitted 8 new biobricks to the registry this year.</p>
+				<html><a target="_blank" href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=uOttawa"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/2/2d/Parts_uo.png') no-repeat !important;"><span>Parts Submitted</span></h2></a></html><p>We submitted 8 new BioBricks to the registry this year.</p>
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-				<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/0/0d/Human_uo.png') no-repeat !important;"><span>Human Practices</span></h2></html><p>In order for the field of Synthetic Biology to grow, more youth outreach is needed. The uOttawa team created a game to teach simple synbio theory and has arranged high school tutorials for the fall. Also check out our essay on the public view on Synthetic Biology and ways of improving it.</p>
+				<html><a href="/Team:uOttawa/Game"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/0/0d/Human_uo.png') no-repeat !important;"><span>Gears of Evolution</span></h2></a></html><p>The uOttawa team has designed a 10 level game intended to teach and inform high school students about synthetic biology. This game is fully playable on our wiki, the iGEM community page and the Openwetware site Community Bricks. Click on the image above to play it and please give us feedback!</p>
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-				<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/1/1f/Sponsors_uo.png') no-repeat !important;"><span>Sponsors</span></h2></html><p>The uOttawa team would like to thank our sponsors for their generous support this year. Our sponsors graciously supplied us with lab equipment, reagents and funds necessary for the team to compete.</p>
+				<html><a href="/Team:uOttawa/Sponsors"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/1/1f/Sponsors_uo.png') no-repeat !important;"><span>Sponsors</span></h2></a></html><p>The uOttawa team would like to thank our sponsors for their generous support this year. Our sponsors graciously supplied us with lab equipment, reagents and funds necessary for the team to compete.</p>
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