We have added a new section for the "Plasmid backbones for advanced users" of the Parts' Registry: Genome Integration plasmids, where you could find all the plasmids of the miniTn7 BioBrick tool kit. Enjoy!

Data for our characterized new parts

1. pUC18Sfi-miniTn7BB-Gm plasmid (BBa_K510000). This plasmid harbors the basic miniTn7 construct and may be used for integration of BioBrick parts and devices in single copy into the genomes of non-enteric bacteria, in which it is non-replicative, at the conserved attTn7 site.

2. pUC18R6KT-miniTn7BB-Gm plasmid (BBa_K510012): This plasmid harbors the basic miniTn7 construct and may be used for transposition of BioBrick parts and devices into the genome of bacteria not expressing the pir gene (including Enterobacteria), thanks to is R6K replication origin.

3. attTn7 or Tn7 insertion site (BBa_K510022). Recognition and insertion site for the Tn7 transposon and its synthetic derivatives. Developed to promote target-specific Tn7 transposition in hosts not bearing a functional Tn7 insertion site.

We have also submitted these new parts:

1. pUC18SfiI-miniTn7BB-Cm (BBa_K510001). This construct is the result of replacing the gentamycin resistance cassette in pUC18SfiI-miniTn7BB-Gm by a chloramphenicol resistance cassette obtained from pBS1C3 by PCR amplification.

2. pUC18SfiI-miniTn7BB-Km (BBa_K510002): This construct is the result of replacing the gentamycin resistance cassette of pUC18Sfi-miniTn7-Gm by a kanamycin resistance cassette amplified from pSB4K5.

3. pUC18R6KT-miniTn7BB-Cm (BBa_K510013). This construct is the result of replacing the gentamycin resistance cassette in pUC18R6KT-miniTn7BB-Gm by a chloramphenicol resistance cassette obtained from pBS1C3 by PCR amplification with these primers

4. pUC18R6KT-miniTn7BB-Km (BBa_K510014). This construct is the result of replacing the gentamycin resistance cassette in pUC18R6KT-miniTn7BB-Gm by a kanamycin resistance cassette obtained from pBS4K5.

5. pUC18R6KT-miniTn7BB-Gm-RBS+RFP (BBa_K510015). MiniTn7-based reporter for promoter characterization. Promoter insertion at prefix restriction sites results in a transcriptional fusion to RFP. Characterization can be performed in single copy by integrating the miniTn7 element in the genome of the appropriate organism.

6. pUC18R6KT-miniTn7BB-Gm-pBad/araC (BBa_K510016). MiniTn7-based expression vector. Insertion of genes at suffix restriction sites allows arabinose-inducible transcription. Characterization can be performed in single copy by integrating the miniTn7 element in the genome of the appropriate organism.

7. pUC18R6KT-miniTn7BB-Gm-tetR (BBa_K510017). MiniTn7-based expression vector. Insertion of genes at suffix restriction sites allows oxytetracycline-inducible transcription. Characterization can be performed in single copy by integrating the miniTn7 element in the genome of the appropriate organism.

8. pUC18R6KT-miniTn7BB-Gm-lacZ+GFP (BBa_K510018). MiniTn7-based reporter for promoter characterization. Promoter insertion at prefix restriction sites results in a transcriptional fusion to lacZ alpha fragment and GFP. Characterization can be performed in single copy by integrating the miniTn7 element in the genome of the appropriate organism.

9. pUC18R6KT-miniTn7BB-Gm-RFP (BBa_K510020). MiniTn7-based vector for fluorescent labeling of bacterial strains. Single copy labeling with constitutively-produced RFP is achieved by integrating the miniTn7 element in the genome of the appropriate organism.

10. pUC18R6KT-miniTn7BB-Gm-Lux (BBa_K510021): MiniTn7-based vector for biomoluminescent labeling of bacterial strains. Single copy labeling with LuxAB is achieved by integrating the miniTn7 element in the genome of the appropriate organism.