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Team:Nevada/Notebook
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Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion. | Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion. | ||
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| - | <ul>Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP | + | <ul>Rxn: |
| + | <li>1. glucose + ATP --Hx-> G-6-P + ADP</li> | ||
<li>2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH</li> | <li>2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH</li> | ||
</ul> | </ul> | ||
Revision as of 19:27, 30 June 2011
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E. Coli Cyano Enzymology Media
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E. Coli
Blah Blah Blah
Cyano
Blah Blah Blah
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
- Rxn:
- 1. glucose + ATP --Hx-> G-6-P + ADP
- 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH
Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme.
Media
