Team:Edinburgh/Cell Display

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Revision as of 15:43, 30 June 2011

An obvious type of bioreactor is an E. coli cell that displays the desired proteins on its outer membrane. This type of display is called cell surface display.

This works by fusing the protein of interest to a carrier protein which is naturally found on the outer membrane.

Notes

<partinfo>BBa_K265008</partinfo> is the first 211 and last 97 amino acids of ice nucleation protein (INP, coded by [http://www.uniprot.org/uniprot/O30611 inaK] gene) from Pseudomonas syringae. It seems promising as a carrier of enzymes. [http://www.sciencedirect.com/science/article/pii/S016777991000199X Van Bloois et al (2011)] speak highly of INP. Fusions are carried out at the INP C-terminal.

Berkeley 2009 Parts may be helpful: they tried several different carrier proteins. When they tried attaching cellulases, they weren't too successful - of the two quantified cellulases, one worked just as well without the carrier (Cel5b) and the other didn't work (Cel9a, as compared to negative control).

References

Van Bloois E, Winter RT, Kolmar H, Fraaije MW (2011) [http://www.sciencedirect.com/science/article/pii/S016777991000199X Decorating microbes: surface display of proteins on Escherichia coli]. Trends in Biotechnology 29(2): 79-86 (doi: 10.1016/j.tibtech.2010.11.003).

This was the old Navbox for Edinburgh; now it's obsolete...

Edinburgh 2011

Project documentation: Project - BioSandwich - Parts - Modeling - Lab Notebook - Safety - Team - Attributions

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 <partinfo>BBa_K265008</partinfo> is the first 211 and last 97 amino acids of ice nucleation protein (INP, coded by ''[http://www.uniprot.org/uniprot/O30611 inaK]'' gene) from ''Pseudomonas syringae''. It seems promising as a carrier of enzymes. [http://www.sciencedirect.com/science/article/pii/S016777991000199X Van Bloois ''et al'' (2011)] speak highly of INP. Fusions are carried out at the INP C-terminal.
-[[Team:Edinburgh/Berkeley 2009 Parts|Berkeley 2009 Parts]] may be helpful: they tried several different carrier proteins. When they tried attaching cellulases, they [https://2009.igem.org/Team:Berkeley_Wetlab/Passenger:_Cellulases weren't too successful] - of the two quantified cellulases, one worked even without the carrier (Cel5b) and the other didn't work (Cel9a, as compared to negative control).
+[[Team:Edinburgh/Berkeley 2009 Parts|Berkeley 2009 Parts]] may be helpful: they tried several different carrier proteins. When they tried attaching cellulases, they [https://2009.igem.org/Team:Berkeley_Wetlab/Passenger:_Cellulases weren't too successful] - of the two quantified cellulases, one worked just as well without the carrier (Cel5b) and the other didn't work (Cel9a, as compared to negative control).
 ==References==