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Team:Tec-Monterrey/projectmodeling/construct3
From 2011.igem.org
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<h2>PROJECT</h2> | <h2>PROJECT</h2> | ||
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| - | + | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectoverview">overview</a></p> | |
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<p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectparts">parts</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectparts">parts</a></p> | ||
<p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">genetic frame</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">genetic frame</a></p> | ||
| - | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults">methods | + | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults/methods">methods</a></p> |
| + | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults">results</a></p> | ||
<p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human approach</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human approach</a></p> | ||
<p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectprotocols">protocols</a><p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectprotocols">protocols</a><p> | ||
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<p class="textojustif"> | <p class="textojustif"> | ||
| - | Our third construct ompA+sacC has the same promoter as the last two (P<sub>BAD</sub> promoter, <a href="http://partsregistry.org/Part:BBa_K206000"> BBa_K206000</a>, and its repressor protein araC, <a href="http://partsregistry.org/Part:BBa_I13458">BBa_I13458 </a>) We decided to use an already reported part, membrane protein ompA and a signal peptide of lpp (<a href="http://partsregistry.org/Part:BBa_K103006">BBa_K103006</a>). We decided to try out our extracellular invertase “sacC” (<a href="http://partsregistry.org/Part:BBa_K633003">BBa_K633003</a>) cloned out of <i>Zymmomonas Mobilis</i> in our university. The importance behind this part is that it’s a monomeric enzyme, able to produce both glucose and fructose from sucrose, an important approach for inverted sugar. | + | Our third construct ompA+sacC has the same promoter as the last two (P<sub>BAD</sub> promoter, <a href="http://partsregistry.org/Part:BBa_K206000">BBa_K206000</a>, and its repressor protein araC, <a href="http://partsregistry.org/Part:BBa_I13458">BBa_I13458</a>) We decided to use an already reported part, membrane protein ompA and a signal peptide of lpp (<a href="http://partsregistry.org/Part:BBa_K103006">BBa_K103006</a>). We decided to try out our extracellular invertase “sacC” (<a href="http://partsregistry.org/Part:BBa_K633003">BBa_K633003</a>) cloned out of <i>Zymmomonas Mobilis</i> in our university. The importance behind this part is that it’s a monomeric enzyme, able to produce both glucose and fructose from sucrose, an important approach for inverted sugar. |
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Latest revision as of 20:50, 20 October 2011
Our third construct ompA+sacC has the same promoter as the last two (PBAD promoter, BBa_K206000, and its repressor protein araC, BBa_I13458) We decided to use an already reported part, membrane protein ompA and a signal peptide of lpp (BBa_K103006). We decided to try out our extracellular invertase “sacC” (BBa_K633003) cloned out of Zymmomonas Mobilis in our university. The importance behind this part is that it’s a monomeric enzyme, able to produce both glucose and fructose from sucrose, an important approach for inverted sugar.
Kyono K, Yanase H, Tonomura K, Kawasaki H and Sakai T. (1995) Cloning and characterization of Zymomonas mobilis genes encoding extracellular levansucrase and invertase. Biosci Biotechnol Biochem. Vol 59 (2):289-293
Kannan R, Mukundan G, Ait-Abdelkader N, Augier-Magro V, Baratti J and Gunasekaran P. (1995) Molecular cloning and characterization of the extracellular sucrose gene (sacC) of Zymomonas mobilis” Arch. Microbiol. Vol 163(3):195-204
