Team:Tec-Monterrey/projectmodeling/construct3

From 2011.igem.org

(Difference between revisions)
 
(12 intermediate revisions not shown)
Line 63: Line 63:
body {
body {
font: 12px Tahoma, Geneva, sans-serif;
font: 12px Tahoma, Geneva, sans-serif;
-
background-image: url(https://static.igem.org/mediawiki/igem.org/a/aa/Posiblefondo01.png);
+
background-image: url(https://static.igem.org/mediawiki/2011/4/43/Lefondoleposter.png);
         background-repeat:no-repeat;
         background-repeat:no-repeat;
       background-size: 100%;
       background-size: 100%;
Line 516: Line 516:
   <h2>PROJECT</h2>
   <h2>PROJECT</h2>
     <div class="panelcontent" style="">
     <div class="panelcontent" style="">
-
   
+
        <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectoverview">overview</a></p>
-
        <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectoverview">overview</a></p>
+
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectparts">parts</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectparts">parts</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">genetic frame</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">genetic frame</a></p>
-
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/safetypage">safety</a></p>
+
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults/methods">methods</a></p>
-
            <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human practice</a></p>
+
-
            <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook">notebook</a></p>
+
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults">results</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults">results</a></p>
 +
            <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human approach</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectprotocols">protocols</a><p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectprotocols">protocols</a><p>
 +
            <p><a href="https://2011.igem.org/Team:Tec-Monterrey/safetypage">safety</a></p>
 +
            <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook">notebook</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/sampledata">sample data</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/sampledata">sample data</a></p>
           </div>
           </div>
Line 556: Line 556:
     </div>
     </div>
  </div>
  </div>
-
       </li>
+
       </li> </ul>
-
 
+
-
</ul>
+
    
    
</div>
</div>
Line 567: Line 565:
   
   
<a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">  
<a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">  
-
<img src="https://static.igem.org/mediawiki/2011/8/8f/Construct01.png"></a>
+
<img src="https://static.igem.org/mediawiki/2011/e/e9/Construct01_a.png"></a>
<a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling/construct2" >
<a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling/construct2" >
-
<img src="https://static.igem.org/mediawiki/2011/f/fe/Construct02.png
+
<img src="https://static.igem.org/mediawiki/2011/4/47/Construct02_a.png"></a>
-
"></a>
+
<a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling/construct3" >
<a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling/construct3" >
Line 592: Line 589:
<div class="frame" id="frame1" style="background-color:#e5e5e5;">
<div class="frame" id="frame1" style="background-color:#e5e5e5;">
<br>
<br>
-
      
+
<center>
 +
     <p><img src="https://static.igem.org/mediawiki/2011/9/91/Partes02.png" alt="photo3" name="photo3" width="400" id="photo3" /><br />
 +
      </p>
 +
</center> 
 +
 
 +
 
 +
<br><br>
 +
    <p class="textojustif">
 +
Our third construct ompA+sacC has the same promoter as the last two (P<sub>BAD</sub> promoter, <a href="http://partsregistry.org/Part:BBa_K206000">BBa_K206000</a>, and its repressor protein araC, <a href="http://partsregistry.org/Part:BBa_I13458">BBa_I13458</a>) We decided to use an already reported part, membrane protein ompA and a signal peptide of lpp (<a href="http://partsregistry.org/Part:BBa_K103006">BBa_K103006</a>). We decided to try out our extracellular invertase “sacC” (<a href="http://partsregistry.org/Part:BBa_K633003">BBa_K633003</a>) cloned out of <i>Zymmomonas Mobilis</i> in our university. The importance behind this part is that it’s a monomeric enzyme, able to produce both glucose and fructose from sucrose, an important approach for inverted sugar.
 +
<br>
 +
<br>
 +
 
 +
 
 +
 
 +
<center><img src="https://static.igem.org/mediawiki/2011/6/66/Referencesimg.png" alt="" name="" width="200" height="50" id="tgo"></center>
 +
<br>
 +
<p class="textojustif">
-
Our third Construct has the same promoter as the last two (Pbad promoter, BBa_K206000, and its repressor enzyme AraC, BBa_I13458) We decided to use an already reported part, membrane protein ompA and its signal peptide lpp (BBa_K103006) We decided to try out our extracellular invertase “Sacc” (BBa_K633003) cloned out of Zymmomonas Mobilis in our university. The importance behind this part is that it’s a monomeric enzyme, able to produce both glucose and fructose from sucrose, an important approach for high fructose syrups.
+
Kyono K, Yanase H, Tonomura K, Kawasaki H and Sakai T. (1995) Cloning and characterization of <i>Zymomonas mobilis</i> genes encoding extracellular levansucrase and invertase. Biosci Biotechnol Biochem. Vol 59 (2):289-293
<br>
<br>
-
Kyono,K., Yanase,H., Tonomura,K., Kawasaki,H. and Sakai,T.”Cloning and characterization of Zymomonas mobilis genes encoding extracellular levansucrase and invertase” Biosci. Biotechnol. Biochem. 59 (2), 289-293 (1995)
 
<br>
<br>
-
Kannan,R., Mukundan,G., Ait-Abdelkader,N., Augier-Magro,V., Baratti,J. and Gunasekaran,P.”Molecular cloning and characterization of the extracellular sucrose gene (sacC) of Zymomonas mobilis” Arch. Microbiol. 163 (3), 195-204 (1995)
+
Kannan R, Mukundan G, Ait-Abdelkader N, Augier-Magro V, Baratti J and Gunasekaran P. (1995) Molecular cloning and characterization of the extracellular sucrose gene (sacC) of <i>Zymomonas mobilis</i>” Arch. Microbiol. Vol 163(3):195-204  
<br>
<br>

Latest revision as of 20:50, 20 October 2011

wiki

iGEM

 

 


photo3



Our third construct ompA+sacC has the same promoter as the last two (PBAD promoter, BBa_K206000, and its repressor protein araC, BBa_I13458) We decided to use an already reported part, membrane protein ompA and a signal peptide of lpp (BBa_K103006). We decided to try out our extracellular invertase “sacC” (BBa_K633003) cloned out of Zymmomonas Mobilis in our university. The importance behind this part is that it’s a monomeric enzyme, able to produce both glucose and fructose from sucrose, an important approach for inverted sugar.


Kyono K, Yanase H, Tonomura K, Kawasaki H and Sakai T. (1995) Cloning and characterization of Zymomonas mobilis genes encoding extracellular levansucrase and invertase. Biosci Biotechnol Biochem. Vol 59 (2):289-293

Kannan R, Mukundan G, Ait-Abdelkader N, Augier-Magro V, Baratti J and Gunasekaran P. (1995) Molecular cloning and characterization of the extracellular sucrose gene (sacC) of Zymomonas mobilis” Arch. Microbiol. Vol 163(3):195-204




Retrieved from "http://2011.igem.org/Team:Tec-Monterrey/projectmodeling/construct3"