Team:Tec-Monterrey/projectnotebook june/day06
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<p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">modeling</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">modeling</a></p> | ||
<p><a href="https://2011.igem.org/Team:Tec-Monterrey/safetypage">safety</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/safetypage">safety</a></p> | ||
- | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human | + | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human practice</a></p> |
<p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook">notebook</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook">notebook</a></p> | ||
<p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults">results</a></p> | <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults">results</a></p> | ||
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- | <a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook_june/ | + | <a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook_june/day03"> |
<img src="https://static.igem.org/mediawiki/2011/c/c8/Botonleft01.png"> </a> | <img src="https://static.igem.org/mediawiki/2011/c/c8/Botonleft01.png"> </a> | ||
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<br> | <br> | ||
- | < | + | <p class="justtext"> |
+ | 06/June/2011 | ||
+ | </br> | ||
+ | • miniprep of 22P (103.2 NG/ µ L ) and ( 114 ng / µ L ) | ||
+ | </br> | ||
+ | • Digestions | ||
+ | </br> | ||
+ | • Sterilize material | ||
+ | </br> | ||
+ | • Preparation of Km and Ap stock. | ||
+ | </br> | ||
+ | • Electrophoresis | ||
+ | </br> | ||
+ | • Transformation in the microwave | ||
+ | </br> | ||
+ | • 22 P + 2 M -> in a plate with Ap | ||
+ | </br> | ||
+ | • 16 K + 20 B + 14 N -> in plates with Km | ||
+ | </br> | ||
+ | • We chose one colony of the 22P + 2 M and put it in 10 ml LB media + 100 | ||
+ | µg / mL) | ||
+ | </p> | ||