Team:Tec-Monterrey/projectnotebook june/day06

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             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">modeling</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">modeling</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/safetypage">safety</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/safetypage">safety</a></p>
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             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human practices</a></p>
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             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human practice</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook">notebook</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook">notebook</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults">results</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectresults">results</a></p>
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<a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook_june/day05">
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<a href="https://2011.igem.org/Team:Tec-Monterrey/projectnotebook_june/day03">
<img src="https://static.igem.org/mediawiki/2011/c/c8/Botonleft01.png"> </a>
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<br>
<br>
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<img src="https://static.igem.org/mediawiki/2011/8/80/Comingsoon.png">
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<p class="justtext">
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06/June/2011
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</br>
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• miniprep of 22P (103.2 NG/ µ L ) and ( 114 ng / µ L )
 +
</br>
 +
• Digestions
 +
</br>
 +
• Sterilize material
 +
</br>
 +
• Preparation of Km and Ap stock.
 +
</br>
 +
• Electrophoresis
 +
</br>
 +
• Transformation in the microwave
 +
</br>
 +
• 22 P + 2 M -> in a plate with Ap
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</br>
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• 16 K + 20 B + 14 N -> in plates with Km
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</br>
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• We chose one colony of the 22P + 2 M and put it in 10 ml LB media + 100
 +
µg / mL)
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</p>

Latest revision as of 03:38, 18 October 2011

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iGEM

 

 


06/June/2011
• miniprep of 22P (103.2 NG/ µ L ) and ( 114 ng / µ L )
• Digestions
• Sterilize material
• Preparation of Km and Ap stock.
• Electrophoresis
• Transformation in the microwave
• 22 P + 2 M -> in a plate with Ap
• 16 K + 20 B + 14 N -> in plates with Km
• We chose one colony of the 22P + 2 M and put it in 10 ml LB media + 100 µg / mL)