Team:Tec-Monterrey/projectnotebook august/day03

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Latest revision as of 00:35, 18 October 2011

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iGEM

 

 


03/August/2011
• Sergio gave us some recommendations about bacteria induction.
• Min inoculum 13L, 8G, 2M, 22P, sac and 24C to have an stock of cells and minipreps of construct B
• Inoculum of DH5-α, sac, pET12a to verify the restriction site of EcoRI.
• IPTG induction of 6 different strains: C41, C43, BW27783, XLIBlue, BL21 and Rosseta gami.
• We pick 2 colonies of each strain for overnight
• Minipreps of yesterday’s stock
• Digestion with EcoRI and PstI forward electrophoresis.


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