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Team:Edinburgh/Cell Display
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An obvious type of bioreactor is an ''E. coli'' cell that displays the desired proteins on its outer membrane. This type of display is called '''cell surface display'''. | An obvious type of bioreactor is an ''E. coli'' cell that displays the desired proteins on its outer membrane. This type of display is called '''cell surface display'''. | ||
| + | This works by fusing the protein of interest to | ||
==Notes== | ==Notes== | ||
| - | <partinfo>BBa_K265008</partinfo> is the | + | <partinfo>BBa_K265008</partinfo> is the first 211 and last 97 amino acids of ice nucleation protein (INP, coded by ''[http://www.uniprot.org/uniprot/O30611 inaK]'' gene) from ''Pseudomonas syringae''. It seems promising as a carrier of enzymes. [http://www.sciencedirect.com/science/article/pii/S016777991000199X Van Bloois ''et al'' (2011)] speak highly of INP. |
[[Team:Edinburgh/Berkeley 2009 Parts|Berkeley 2009 Parts]] may be helpful: they tried several different carrier proteins. | [[Team:Edinburgh/Berkeley 2009 Parts|Berkeley 2009 Parts]] may be helpful: they tried several different carrier proteins. | ||
Revision as of 13:42, 30 June 2011
An obvious type of bioreactor is an E. coli cell that displays the desired proteins on its outer membrane. This type of display is called cell surface display.
This works by fusing the protein of interest to
Notes
<partinfo>BBa_K265008</partinfo> is the first 211 and last 97 amino acids of ice nucleation protein (INP, coded by [http://www.uniprot.org/uniprot/O30611 inaK] gene) from Pseudomonas syringae. It seems promising as a carrier of enzymes. [http://www.sciencedirect.com/science/article/pii/S016777991000199X Van Bloois et al (2011)] speak highly of INP.
Berkeley 2009 Parts may be helpful: they tried several different carrier proteins.
References
- Van Bloois E, Winter RT, Kolmar H, Fraaije MW (2011) [http://www.sciencedirect.com/science/article/pii/S016777991000199X Decorating microbes: surface display of proteins on Escherichia coli]. Trends in Biotechnology 29(2): 79-86 (doi: 10.1016/j.tibtech.2010.11.003).
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