"
Template:Protocols
From 2011.igem.org
| Line 9: | Line 9: | ||
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/1/19/Ubcsmilie.jpg" > | <img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/1/19/Ubcsmilie.jpg" > | ||
<img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/b/bb/Ubcigemhearttips.jpg" > | <img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/b/bb/Ubcigemhearttips.jpg" > | ||
| - | + | <img align="left" style="margin-bottom:10px; padding:0;" src="https://static.igem.org/mediawiki/2011/4/42/Ubcigembeetlemini2.jpg" > | |
Revision as of 23:45, 14 October 2011
iGEM Tips for Success
Things to think about when designing your project and experiments, as well as general safety rules.
Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.
Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.
Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.
Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.
Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.
Yeast-Fungi Co-culture Experiments
Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.
