Team:UIUC-Illinois

From 2011.igem.org

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          <div class="title">E.chiver - Dynamic Efficient Bacteria</div>
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      <div class="title"><center>E.chiver - Dynamically Efficient Bacteria:</center></div>
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           <div class="desc"><center>Creating a "Bacterial Filing Cabinet"</center></div>
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        <div id="quick-feed-read-more"><center>1 &nbsp; 2 &nbsp; 3 &nbsp; 4 &nbsp; 5</center></div>
 
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         <div class="title">What is eChiver?</div>
         <div class="title">What is eChiver?</div>
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         <div class="desc"><img src="images/t1-about.jpg" align="left" />This is a quick description, or will be a quick description about the future iFile Project. An ecoli cell that has an all new way of changing the way we think about the function of a cell. Imagine a cell that was fully adaptable to different conditions that would maxamize productivity if needed, but would not waste energy when a cell didn't need to. Consider it the eColi cell version 2.0. The higher the number, the better right? Well, welcome to the iFile Project.</div>
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         <div class="desc"><img src="https://static.igem.org/mediawiki/2011/e/e5/Uiuc_Echiver.jpg" width="112" height="162" align="left" />Our project, E. chiver, drew inspiration from the commonly used CRIM system, a series of plasmids that allows the user to integrate constructs into lambdoid phage sites common to many bacterial chromosomes. Our E. chiver system adds several elements yielding new applications. Our team designed two E. chiver constructs utilizing Lambda and P21 machinery. Each can in theory be used to shuttle a plasmid construct between two forms: a single chromosomal insert and a high copy number plasmid. In their current designs the systems must function separately, but possible routes have been identified by our team to make the co-functioning of these systems possible. We can see elements of our project being used in drug delivery systems as a method to keep a gene of interest dormant unless in the correct condition/location, and with further exploration into the co-functioning routes it may be used to create a ‘bacterial filing cabinet’.</div>
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        <img src="https://static.igem.org/mediawiki/2011/8/8d/Uiuc_lion_dynamic.jpg" align="left" />
         <div class="core-content-block-t2-left-title">Dynamic</div>
         <div class="core-content-block-t2-left-title">Dynamic</div>
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         <div class="desc"><img src="images/t1-about.jpg" align="left" />Responding to stimuli on the fly with rapid changes in state and function.</div>
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         <div class="desc">Responding to stimuli on the fly with rapid changes in state and function.</div>
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        <img src="https://static.igem.org/mediawiki/2011/4/48/Uiuc_lion_efficient.jpg" align="left" />
         <div class="core-content-block-t2-left-title">Efficient</div>
         <div class="core-content-block-t2-left-title">Efficient</div>
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         <div class="desc"><img src="images/t1-about.jpg" align="left" />While maximizing efficiency in both the active and passive states.</div>
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         <div class="desc">While maximizing efficiency in both the active and passive states.</div>
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        <img src="https://static.igem.org/mediawiki/2011/b/b1/Uiuc_lion_multipurpose.jpg" align="left" />
         <div class="core-content-block-t2-left-title">Multi-purpose</div>
         <div class="core-content-block-t2-left-title">Multi-purpose</div>
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         <div class="desc"><img src="images/t1-about.jpg" align="left" />A variety of possible stimuli that each induce a different response.</div>
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         <div class="desc">A variety of possible stimuli that each induce a different response.</div>
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        <div id="sponsors-nano"><a href="http://mntl.illinois.edu/"><img src="https://static.igem.org/mediawiki/2011/c/ca/Uiuc2011_sponsor_micro_nano_lab.jpg" alt="Micro and Nanotechnology Laboratory" /></a></div>
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          <div id="sponsors-nano"><a href="http://mntl.illinois.edu/"><img src="https://static.igem.org/mediawiki/2011/c/ca/Uiuc2011_sponsor_micro_nano_lab.jpg" alt="Micro and Nanotechnology Laboratory" /></a></div>
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         <div id="sponsors-labcon"><a href="http://www.labcon.com/"><img src="https://static.igem.org/mediawiki/2011/e/e8/Uiuc2011_sponsor_labcon.jpg" alt="Labcon North America" /></a></div>
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          <div id="sponsors-idt"><a href="http://www.idtdna.com/"><img src="https://static.igem.org/mediawiki/2011/c/c1/Uiuc2011_sponsor_idt.jpg" alt="Integrated DNA Technologies" /></a></div>
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          <div id="sponsors-genscript"><a href="http://www.genscript.com/"><img src="https://static.igem.org/mediawiki/2011/a/a1/Uiuc2011_sponsor_genscript.jpg" alt="GenScript" /></a></div>
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         <div id="sponsors-usscien"><a href="http://www.usascientific.com/"><img src="https://static.igem.org/mediawiki/2011/8/8f/Uiuc2011_sponsor_usa_scientific.jpg" alt="USA Scientific" /></a></div>
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         <div id="sponsors-abe"><a href="http://abe.illinois.edu/"><img src="https://static.igem.org/mediawiki/2011/8/84/Uiuc2011_sponsor_abe.jpg" alt="Department of Agricultural and Biological Engineering" /></a></div>
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         <div id="sponsors-midsci"><a href="http://www.midsci.com/"><img src="https://static.igem.org/mediawiki/2011/8/82/Uiuc2011_sponsor_midsci.jpg" alt="MidSci" /></a></div>
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         <div id="sponsors-abe"><a href="http://www.igb.uiuc.edu/"><img src="https://static.igem.org/mediawiki/2011/7/7c/Uiuc2011_sponsor_igb.jpg" alt="The Institute for Genomic Biology" /></a></div>
   
   
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<center><a href="https://www.vwrsp.com/"><font color="white">VWR</font></a> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; <a href="http://www.fishersci.com/"><font color="white">Fisher Scientific</font></a> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;  <a href="http://www.labcon.com/"><font color="white"> labcon </font></a></center>
   
   
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Latest revision as of 02:02, 14 October 2011

University of Illinois iGEM Team
Echiver_logo
E.chiver - Dynamically Efficient Bacteria:
Creating a "Bacterial Filing Cabinet"
University of Illinois - International Genetically Engineered Machine
What is eChiver?
Our project, E. chiver, drew inspiration from the commonly used CRIM system, a series of plasmids that allows the user to integrate constructs into lambdoid phage sites common to many bacterial chromosomes. Our E. chiver system adds several elements yielding new applications. Our team designed two E. chiver constructs utilizing Lambda and P21 machinery. Each can in theory be used to shuttle a plasmid construct between two forms: a single chromosomal insert and a high copy number plasmid. In their current designs the systems must function separately, but possible routes have been identified by our team to make the co-functioning of these systems possible. We can see elements of our project being used in drug delivery systems as a method to keep a gene of interest dormant unless in the correct condition/location, and with further exploration into the co-functioning routes it may be used to create a ‘bacterial filing cabinet’.
Who We Are
Amanda Chang
"A watched gel never runs"
Dynamic
Responding to stimuli on the fly with rapid changes in state and function.
Efficient
While maximizing efficiency in both the active and passive states.
Multi-purpose
A variety of possible stimuli that each induce a different response.
Sponsors
Micro and Nanotechnology Laboratory
Integrated DNA Technologies
GenScript
USA Scientific
Department of Agricultural and Biological Engineering
The Institute for Genomic Biology
VWR           Fisher Scientific           labcon

Retrieved from "http://2011.igem.org/Team:UIUC-Illinois"