Team:Amsterdam

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<meta name="description" content="iGEM team Amsterdam 2011. In this wiki you can find information on our progress in the iGEM competition. Our labjournal, recent activities, biobricks and much more." />
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<h1>iGEM team Amsterdam 2011                           <g:plusone></g:plusone></h1>
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<h1>Welcome                           <g:plusone></g:plusone></h1>
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Welcome to the wiki of team iGEM Amsterdam 2011! On this site, you can find out everything about the team that represents Amsterdam in this year's edition of the iGEM competition, as well as our project: <a href="https://2011.igem.org/Team:Amsterdam/Project/Description"><b>icE. coli</b></a>. You will find answers to questions like...<br><br>
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Welcome to the <a href="https://2011.igem.org/Main_Page">iGEM</a> 2011 Amsterdam wiki!
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Our <a href="https://2011.igem.org/Team:Amsterdam/Team/Students">team</a> consists of students from the <a href="http://www.english.uva.nl/start.cfm">University of Amsterdam</a> and the <a href="http://www.vu.nl/en/index.asp"> VU University Amsterdam</a>.
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On this site you can find everything that has to do with our project: <a href="https://2011.igem.org/Team:Amsterdam/Project/Description"><b>icE. coli</b></a>. You will also find information on:<br><br>
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<ul><li>Who are the <a href="https://2011.igem.org/Team:Amsterdam/Team/Students">team members</a>?</li>
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<ul><li>Our <a href="https://2011.igem.org/Team:Amsterdam/Human_Outreach/Overview">human outreach</a>
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<li>What are they <a href="https://2011.igem.org/Team:Amsterdam/Project/Description">working</a> on?</li>
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<li>Our <a href="https://2011.igem.org/Team:Amsterdam/Sponsors/Companies">sponsors</a>
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<li>Who's <a href="https://2011.igem.org/Team:Amsterdam/Sponsors">paying</a> for all this?</li></ul>
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<li>The <a href="https://2011.igem.org/Team:Amsterdam/Notebook/Overview">day-to-day labwork</a><br>
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... and many more!<br><br>
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<li>... and lots of other information relating to our project.</li></ul><br>
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<b>Want a quick no-nonsense tour of our project? Visit the <a href=https://2011.igem.org/Team:Amsterdam/Project/Data>data page</a>.</b>
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Please take a look around, and be sure to <A HREF="mailto:igemamsterdam@gmail.com">contact us</a> with any questions you are left with!
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<br><br>
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Please take a look around, and be sure to <A HREF="mailto:igemamsterdam@gmail.com">contact us</a> with any questions!
<br><br>
<br><br>
<h1>Abstract</h1>
<h1>Abstract</h1>
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<i>Escherichia coli</i>'s optimal growth temperature is 37°C. When decreasing the temperature the growth rate of the bacterium decreases rapidly. Once it reaches 7 °C, growth of wild type '<i>E. coli</i> is impossible.<br>
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<i>Escherichia coli's</i> optimal growth temperature is 37°C. Growth rate decreases drastically at lower temperatures, and completely halts below 7°C. Our project aims to increase <i>E. coli’s</i> cold tolerance, by expressing several chaperone proteins normally found in psychrophillic bacteria. Chaperone proteins regulate structure of biological molecules, like proteins and RNA, in some cases safeguarding their function at low temperatures. By expressing psychrophillic chaperones, we aim to enhance <i>E. coli's</i> cold resistance in two ways. Growth rate is increased at temperatures below 37°C, possibly even enabling growth at 0°C, and <i>E. coli’s</i> ability to survive freezing environments is enhanced. Expression of psychrophile chaperones is achieved through BioBricks, combining promoters and ribosome binding sites characterised by previous iGEM teams with newly synthesized coding regions. Our team has successfully expressed two psychrophile chaperones in <i>E. coli</i>. We demonstrate CspC enhances the survival rate of frozen <i>E. coli</i>, and investigate putative novel functions of Cpn10. <br><br>
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<h1>Medal requirements</h1>
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<td class="title">Bronze</td>
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<td class="middletitle">Silver</td>
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<td class="title">Gold</td>
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<td><a href="https://igem.org/Team.cgi?id=538"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Team registration</a></td>
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<td class="middle"><a href="https://2011.igem.org/Team:Amsterdam/Labwork/Characterization"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Working BioBricks</a></td>
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<td><a href="https://2011.igem.org/Team:Amsterdam/Human_Outreach/Collaboration"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Collaboration</a></td>
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<td><a href="https://igem.org/2011_Judging_Form?id=538"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Complete judging form</a></td>
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<td class="middle"><a href="https://2011.igem.org/Team:Amsterdam/Labwork/Characterisation"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">BioBrick characterization</a></td>
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<td><a href="https://2011.igem.org/Team:Amsterdam/Human_Outreach/Overview"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Human outreach</a></td>
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<td><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Team Wiki</td>
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<td><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Present at iGEM Jamboree</td>
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<td><a href="https://2011.igem.org/Team:Amsterdam/Biobricks/Overview#Registry_Submissions"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">New BioBricks submitted</td>
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In our project, we will try to increase the cold tolerance of <i>E. coli</i>, allowing it to grow faster at temperatures below 37°C. At the end of our project, we expect to be able to culture our bacteria at very low temperatures, possibly even at or below freezing point.<br>
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<h1>News</h1>
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The approach we use involves heterologous expression of proteins in the DH5α <i>E. coli</i> strain. Out of different (combinations of) synthesized genes and parts submitted by previous iGEM teams, we will create cold resistance BioBricks – also known as CryoBricks. <br>
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These CryoBricks will be valuable to both fundamental and applied research. One of the many applications is replacing antibiotic resistance with cold resistance as a selection property in plasmid backbones. They can also be useful in various projects of other iGEM teams, and may open new avenues for heterologous expression of heat-sensitive proteins.
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Would you like to contact us? <A HREF="mailto:igemamsterdam@gmail.com">E-Mail</A>
Would you like to contact us? <A HREF="mailto:igemamsterdam@gmail.com">E-Mail</A>
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<a href="http://twitter.com/igemamsterdam" class="twitter-follow-button" data-show-count="yes">Follow @igemamsterdam</a>
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Would you like to help us?
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<a href="http://www.indiegogo.com/iGEM-Amsterdam-icE-coli">Donate here!</a><br>
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<a href="http://www.indiegogo.com/iGEM-Amsterdam-icE-coli"> <img src="https://static.igem.org/mediawiki/2011/5/56/Indiegogo_logo3.jpg" style= "max-width:250px;" /> </a>
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Latest revision as of 21:20, 7 October 2011

Welcome

Welcome to the iGEM 2011 Amsterdam wiki! Our team consists of students from the University of Amsterdam and the VU University Amsterdam. On this site you can find everything that has to do with our project: icE. coli. You will also find information on:


Want a quick no-nonsense tour of our project? Visit the data page.

Please take a look around, and be sure to contact us with any questions!

Abstract

Escherichia coli's optimal growth temperature is 37°C. Growth rate decreases drastically at lower temperatures, and completely halts below 7°C. Our project aims to increase E. coli’s cold tolerance, by expressing several chaperone proteins normally found in psychrophillic bacteria. Chaperone proteins regulate structure of biological molecules, like proteins and RNA, in some cases safeguarding their function at low temperatures. By expressing psychrophillic chaperones, we aim to enhance E. coli's cold resistance in two ways. Growth rate is increased at temperatures below 37°C, possibly even enabling growth at 0°C, and E. coli’s ability to survive freezing environments is enhanced. Expression of psychrophile chaperones is achieved through BioBricks, combining promoters and ribosome binding sites characterised by previous iGEM teams with newly synthesized coding regions. Our team has successfully expressed two psychrophile chaperones in E. coli. We demonstrate CspC enhances the survival rate of frozen E. coli, and investigate putative novel functions of Cpn10.

Medal requirements


News

22-09-2011

Wikifreeze

We have finished our website and are now getting ready for an entire week focussing on the presentation, poster and all the other preparations. In a couple of hours all the European wiki's will be frozen, so we will not be able to change anything till the 3th of October. Luckily for us we have confirmed that our icE.coli will survive this wiki freeze. Hopefully we will see you all in Amsterdam next week.

21-09-2011

Media attention by Bionieuws

We got media attention by the magizine for biologists: Bionieuws. Every two weeks, new and exciting bionews is published. This time, Bionieuws has an article dedicated to the iGEM competition, iGEM team Amsterdam and the project IcE. coli.

20-09-2011

Interview with Robbert Dijkgraaf

Today two of our teammembers went to the UvA for an interview with Robbert Dijkgraaf. Dijkgraaf is a professor in Mathematical Physics at the University of Amsterdam and president of the Royal Netherlands Academy of Arts and Sciences. We talked to him about Synthetic Biology: the dangers and the impact on society, but also about what scientists can do to involve the public in the debate. More information on this interview can be found here

18-09-2011

Media attention by C2W and Elsevier Science

Again, we got media attention by two scientific magazines: C2W and Elsevier Science. Read the article of C2W here and that of Elsevier Science here .

16-09-2011

Last day of biobrickbuilding

Today was the last day to finish the constructs. All our parts have been sent to the iGEM headquarters and the last growth experiments have started. Next week we will focus on collecting the last results and finishing the wiki before the wikifreeze next wednesday.

13-09-2011

Media attention by Ad Valvas

One of our university papers, Ad Valvas, has interviewed us about our project. In the article, members of iGEM team Amsterdam tells the story about our 'cool' bacteria and human outreach projects. Click here to read their story.

01-09-2011

Meeting of Young Minds in Delft

To help the political youth parties with their debate, the iGEM team in Delft, in cooperation with the Rathenau institute, has organized an entire to discuss ethics and iGEM. It was a very interesting day. We'd like to thank Delft for organizing this succesful day. More information on this day can be read here

29-08-2011

Ethics discussion with Dwars

Today, two of our teammates traveled to Utrecht to be present at a meeting of DWARS, the youth department of the political party Groen Links ('Green Left'). The meeting was held to discuss synthetic biology. We started by giving a small presentation on synthetic biology, iGEM and the ethical side of our research. After presenting this information, we participated in a stimulating debate. Overall it was a very fruitful evening and we think that both sides came in touch with interesting new perspectives. More information on this discussion can be found here.

26-08-2011

First succesful assemblies!

After many weeks of trial and error, the first assemblies have succeeded! Armed with fresh construction intermediates, the race is on to make and characterize our CryoBricks!

22-08-2011

Introduction day at faculty VU

Today, the introduction days at the VU started. The freshmen were shown around campus and were introduced to several departments and committees. Our iGEM team arranged to be part of this day in order to have the opportunity to introduce our project to the freshman. Read more...

15-08-2011

Team Amsterdam among favourites

Team Amsterdam is one of the teams to watch out for during the competition, says Christian Lund, a medicine student from Denmark. He participated in last year’s iGEM competition and is now following the progress of the different teams. Read more...

10-08-2011

We now have a newssection. This way you can keep up with the interesting things that happen during our project.



Would you like to contact us? E-Mail



Would you like to help us? Donate here!

Updates

  • 22/09/11 - Wikifreeze: All of our pages have been finalized!
  • 08/09/11 - The BioBricks pages are undergoing a major overhaul!
  • 06/09/11 - Over the last weeks all pages have received updates. Be sure to check them all!
  • 10/08/11 - The Description has been updated!
  • 27/07/11 - The Notebook page has been restructured!
  • 26/07/11 - The Students page has been updated!
  • 19/07/11 - Several meetings and lab journal entries placed onto notebook
  • 13/07/11 - The Project Description and Safety pages have been updated!
  • 08/07/11 - Several meetings placed onto notebook
  • 06/07/11 - Added several lab journal entries
  • 05/07/11 - Updated several bios and notebook
  • 29/06/11 - Several meetings placed onto notebook
  • 28/06/11 - Many small layout changes made
  • 27/06/11 - Several meetings placed onto notebook
  • 24/06/11 - Added notebook calendar
  • 23/06/11 - Fixed sponsor template
  • 21/06/11 - Remaining protocols added
  • 20/06/11 - First protocols added
  • 17/06/11 - Photos added at lab environment
  • 15/06/11 - Created footer
  • 15/06/11 - Created all pages
  • 12/06/11 - Created first version of header
  • 02/06/11 - Temporary wiki online

Locations of visitors to this page

Retrieved from "http://2011.igem.org/Team:Amsterdam"