Team:Kyoto

From 2011.igem.org

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<font size=5>Carnivorous E.coli</font>
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We don't regard E.coli as an animal because they don't hunt or eat.
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==<span class="red_title">Summary</span>==
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Then, if it could hunt and eat, will we think it is an animal?
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We created new E.coli which hunts and eats insects.
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We will create carnivorous E.coli this year.
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As such we named it Carnivorous E.coli.
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Carnivorous E.coli can prey on insects when they are hungry.
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Carnivorous E.coli emits light when it is hungry. This will attract insects to it.
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To explain our project concretely, we made an animation.
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Then, it secretes a viscous substance to capture the insects.
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The captured insects are digested by the chitinase in the secretion.
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Long story short, our Carnivorous E.coli can hunt!
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<!-- https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png -->
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<div id="project_panel">
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    <div>
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<html>
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== <html><a href="/Team:Kyoto/Hunger"><span class="red_title">Team Hunger</span></a></html> ==
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<center>
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<html><div id="hunger"><a href="/Team:Kyoto/Hunger"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html>
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<iframe width="560" height="350" src="https://static.igem.org/mediawiki/2011/f/f0/CarnivorousEcoli.swf" frameborder="0" allowfullscreen></iframe>
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Carnivorous E.coli attracts insects by emitting light, but it is a burden for ''E.coli''.
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</center>
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To reduce this burden, we use nitrogen regulatory proteins, NtrB and NtrC. They activate &sigma;<sup>54</sup> promoter when the supply of nitrogen is not enough.
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</html>
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    </div>
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    <div>
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In this animation, carnivorous E.coli takes four actions; hunger, luminescence, predation and digestion.
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== <html><a href="/Team:Kyoto/Capture"><span class="green_title">Team Capture</span></a></html> ==
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<html><div id="capture"><a href="/Team:Kyoto/Capture"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html>
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There are many methods to attract bugs, for instance, using pheromone, smell, and light.
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<font size=4 > Hunger </font>
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In this study, we focused on light as a method to lure insects.
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Carnivorous E.coli will emit light and attracts bugs like glowworms by using Bioluciferase from <html><a href="https://2010.igem.org/Team:Cambridge">2010 Cambridge</a></html>.
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It is a burden for the E.coli to emit light.
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This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough.
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<font size=4> Luminescence </font>
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There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone.
There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone.
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Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from 2010 Cambridge.
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Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from
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<html><a href="https://2010.igem.org/Team:Cambridge">2010 Cambridge</a></html>.
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-->
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    </div>
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<font size=4> Predation </font>
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== <html><a href="/Team:Kyoto/Digestion"><span class="blue_title">Team Digestion</span></a></html> ==
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<html><div id="digestion"><a href="/Team:Kyoto/Digestion"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html>
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An insect body is covered with a hard integument containing mainly chitin. To decompose the integument, we used ChiA gene, which encode chitinase. In order to measure the chitinase activity of the culture supernatant, we evaluated the effects of the medium and cell growth.
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    </div>
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Carnivorous E.coli traps insects by mucilage.
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#project_panel div {
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Inducing gum gene family, it secrets xanthan gum.
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#hunger, #capture, #digestion {
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#hunger img {margin-left: -2px;}
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#capture img {margin-left: -254px;}
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#digestion img {margin-left: -506px;}
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<font size=4> Digestion </font>
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<div id="under_box">
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Digestion is accomplished by protease and chitinase.  
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==<span class="red_title">Achievement</span>==
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We will induce these enzymes and assay them.
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<ul>
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  <li>We confirmed that the wavelength of light emitted by E.coli is suitable to lure drosophilas.</li>
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  <li>We constructed new biobrick which produces chitinase.</li>
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  <li>We constructed new biobrick that is controlled by the concentration of glutamine.</li>
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  <li>We established new method for measurement of promoter activity with RPU.</li>
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  <li>We held <html><a href="https://2011.igem.org/Summer_events/2011_JAPAN_Meetup">the Japanese iGEM meeting</a></html> at Kyoto University.
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</ul>
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    </div>
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    <div>
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==<html><a href="https://2011.igem.org/Team:Kyoto/HumanPractice"><span class="green_title">Human Practice</span></a></html>==
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As synthetic biology is a comparatively new way of understanding life, we plan to develop this area much further. However, this area entails problems of bioethics and so we must consider these problems as well. Unfortunately, Japanese high school students do not have many chances to come across these topics. We hope to offer high school students more opportunities to think on them. That's why we targeted them and performed these activities.
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    </div>
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    <div>
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==<span class="yellow_title">Sponsors</span>==
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[[File:京大ロゴ.jpg | link=http://www.kyoto-u.ac.jp/|]]
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[[File:CosmoBio.jpg | link=http://www.cosmobio.co.jp |]]
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    </div>
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==Safety==
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*Please use this page to answer the safety questions posed on the [[Safety | safety page]].
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*We are still planning in detail about the experiments. These proposals are not the final ones.
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===Safety of Project===
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Q1: Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or environmental safety?
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A1: Flies are dealt in the same experimental condition with the modified E.coli. <br>
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We will collaborate with the laboratory staff of the university in order to decrease the accidental release of the flies to highlight other risk factors.
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===Safety Rules===
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Q2: Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, did you document these issues in the Registry? how did you manage to handle the safety issue? How could other teams learn from your experience? Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?
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A2: We currently don't think they will raise any safety issues. And we obey the rules of Laboratory of Science Communication and Bioethics, which belongs to the Kyoto University Graduate School of Bioscience in charge of making all the biological researches carried out in our institution transparent to the public.
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===Ideas for the Future===
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Q3: Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
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A3: It is really tough to answer to this question..we'll continue discussing on this fundamental problem..
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Kyoto|Home]]
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!align="center"|[[Team:Kyoto/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Kyoto Official Team Profile]
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!align="center"|[[Team:Kyoto/Project|Project]]
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!align="center"|[[Team:Kyoto/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Kyoto/Modeling|Modeling]]
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!align="center"|[[Team:Kyoto/Notebook|Notebook]]
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!align="center"|[[Team:Kyoto/Safety|Safety]]
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!align="center"|[[Team:Kyoto/Attributions|Attributions]]
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|}
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Latest revision as of 03:58, 6 October 2011

Contents

Summary

We created new E.coli which hunts and eats insects. As such we named it Carnivorous E.coli. Carnivorous E.coli emits light when it is hungry. This will attract insects to it. Then, it secretes a viscous substance to capture the insects. The captured insects are digested by the chitinase in the secretion. Long story short, our Carnivorous E.coli can hunt!

Team Hunger

Carnivorous E.coli attracts insects by emitting light, but it is a burden for E.coli. To reduce this burden, we use nitrogen regulatory proteins, NtrB and NtrC. They activate σ54 promoter when the supply of nitrogen is not enough.

Team Capture

There are many methods to attract bugs, for instance, using pheromone, smell, and light. In this study, we focused on light as a method to lure insects. Carnivorous E.coli will emit light and attracts bugs like glowworms by using Bioluciferase from 2010 Cambridge.

Team Digestion

An insect body is covered with a hard integument containing mainly chitin. To decompose the integument, we used ChiA gene, which encode chitinase. In order to measure the chitinase activity of the culture supernatant, we evaluated the effects of the medium and cell growth.

Achievement

  • We confirmed that the wavelength of light emitted by E.coli is suitable to lure drosophilas.
  • We constructed new biobrick which produces chitinase.
  • We constructed new biobrick that is controlled by the concentration of glutamine.
  • We established new method for measurement of promoter activity with RPU.
  • We held the Japanese iGEM meeting at Kyoto University.

Human Practice

As synthetic biology is a comparatively new way of understanding life, we plan to develop this area much further. However, this area entails problems of bioethics and so we must consider these problems as well. Unfortunately, Japanese high school students do not have many chances to come across these topics. We hope to offer high school students more opportunities to think on them. That's why we targeted them and performed these activities.

Sponsors

京大ロゴ.jpg CosmoBio.jpg

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