Team:Kyoto

From 2011.igem.org

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==<span class="red_title">Summary</span>==
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We created new E.coli which hunts and eats insects.
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As such we named it Carnivorous E.coli.
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Carnivorous E.coli emits light when it is hungry. This will attract insects to it.
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Then, it secretes a viscous substance to capture the insects.
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The captured insects are digested by the chitinase in the secretion.
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Long story short, our Carnivorous E.coli can hunt!
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<!-- https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png -->
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= '''Carnivorous E.coli''' =
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We don't regard E.coli as an animal because they don't hunt or eat.
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== <html><a href="/Team:Kyoto/Hunger"><span class="red_title">Team Hunger</span></a></html> ==
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Then, if it could hunt and eat, will we think it is an animal?
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<html><div id="hunger"><a href="/Team:Kyoto/Hunger"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html>
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We will create carnivorous E.coli this year.
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Carnivorous E.coli attracts insects by emitting light, but it is a burden for ''E.coli''.
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Carnivorous E.coli can prey on insects when they are hungry.
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To reduce this burden, we use nitrogen regulatory proteins, NtrB and NtrC. They activate &sigma;<sup>54</sup> promoter when the supply of nitrogen is not enough.
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To explain our project concretely, we made an animation.
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<iframe width="560" height="350" src="https://static.igem.org/mediawiki/2011/f/f0/CarnivorousEcoli.swf" frameborder="0" allowfullscreen></iframe>
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In this animation, carnivorous E.coli takes four actions; hunger, luminescence, predation and digestion.
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== Hunger ==
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It is a burden for the E.coli to emit light.
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This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough.
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== Luminescence ==
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== <html><a href="/Team:Kyoto/Capture"><span class="green_title">Team Capture</span></a></html> ==
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<html><div id="capture"><a href="/Team:Kyoto/Capture"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html>
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There are many methods to attract bugs, for instance, using pheromone, smell, and light.
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In this study, we focused on light as a method to lure insects.
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Carnivorous E.coli will emit light and attracts bugs like glowworms by using Bioluciferase from <html><a href="https://2010.igem.org/Team:Cambridge">2010 Cambridge</a></html>.
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There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone.
There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone.
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Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from 2010 Cambridge.
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Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from
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<html><a href="https://2010.igem.org/Team:Cambridge">2010 Cambridge</a></html>.
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== Predation ==
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== <html><a href="/Team:Kyoto/Digestion"><span class="blue_title">Team Digestion</span></a></html> ==
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<html><div id="digestion"><a href="/Team:Kyoto/Digestion"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html>
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An insect body is covered with a hard integument containing mainly chitin. To decompose the integument, we used ChiA gene, which encode chitinase. In order to measure the chitinase activity of the culture supernatant, we evaluated the effects of the medium and cell growth.
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Carnivorous E.coli traps insects by mucilage.
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Inducing gum gene family, it secrets xanthan gum.
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== Digestion ==
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Digestion is accomplished by protease and chitinase.  
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==<span class="red_title">Achievement</span>==
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We will induce these enzymes and assay them.
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<ul>
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  <li>We confirmed that the wavelength of light emitted by E.coli is suitable to lure drosophilas.</li>
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  <li>We constructed new biobrick which produces chitinase.</li>
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  <li>We constructed new biobrick that is controlled by the concentration of glutamine.</li>
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  <li>We established new method for measurement of promoter activity with RPU.</li>
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  <li>We held <html><a href="https://2011.igem.org/Summer_events/2011_JAPAN_Meetup">the Japanese iGEM meeting</a></html> at Kyoto University.
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</ul>
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<!--- The Mission, Experiments --->
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==<html><a href="https://2011.igem.org/Team:Kyoto/HumanPractice"><span class="green_title">Human Practice</span></a></html>==
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As synthetic biology is a comparatively new way of understanding life, we plan to develop this area much further. However, this area entails problems of bioethics and so we must consider these problems as well. Unfortunately, Japanese high school students do not have many chances to come across these topics. We hope to offer high school students more opportunities to think on them. That's why we targeted them and performed these activities.
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!align="center"|[[Team:Kyoto|Home]]
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!align="center"|[[Team:Kyoto/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Kyoto Official Team Profile]
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!align="center"|[[Team:Kyoto/Project|Project]]
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!align="center"|[[Team:Kyoto/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Kyoto/Modeling|Modeling]]
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!align="center"|[[Team:Kyoto/Notebook|Notebook]]
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!align="center"|[[Team:Kyoto/Safety|Safety]]
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!align="center"|[[Team:Kyoto/Attributions|Attributions]]
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==<span class="yellow_title">Sponsors</span>==
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[[File:京大ロゴ.jpg | link=http://www.kyoto-u.ac.jp/|]]
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[[File:CosmoBio.jpg | link=http://www.cosmobio.co.jp |]]
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Latest revision as of 03:58, 6 October 2011

Contents

Summary

We created new E.coli which hunts and eats insects. As such we named it Carnivorous E.coli. Carnivorous E.coli emits light when it is hungry. This will attract insects to it. Then, it secretes a viscous substance to capture the insects. The captured insects are digested by the chitinase in the secretion. Long story short, our Carnivorous E.coli can hunt!

Team Hunger

Carnivorous E.coli attracts insects by emitting light, but it is a burden for E.coli. To reduce this burden, we use nitrogen regulatory proteins, NtrB and NtrC. They activate σ54 promoter when the supply of nitrogen is not enough.

Team Capture

There are many methods to attract bugs, for instance, using pheromone, smell, and light. In this study, we focused on light as a method to lure insects. Carnivorous E.coli will emit light and attracts bugs like glowworms by using Bioluciferase from 2010 Cambridge.

Team Digestion

An insect body is covered with a hard integument containing mainly chitin. To decompose the integument, we used ChiA gene, which encode chitinase. In order to measure the chitinase activity of the culture supernatant, we evaluated the effects of the medium and cell growth.

Achievement

  • We confirmed that the wavelength of light emitted by E.coli is suitable to lure drosophilas.
  • We constructed new biobrick which produces chitinase.
  • We constructed new biobrick that is controlled by the concentration of glutamine.
  • We established new method for measurement of promoter activity with RPU.
  • We held the Japanese iGEM meeting at Kyoto University.

Human Practice

As synthetic biology is a comparatively new way of understanding life, we plan to develop this area much further. However, this area entails problems of bioethics and so we must consider these problems as well. Unfortunately, Japanese high school students do not have many chances to come across these topics. We hope to offer high school students more opportunities to think on them. That's why we targeted them and performed these activities.

Sponsors

京大ロゴ.jpg CosmoBio.jpg

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