Team:Kyoto/Protocol

From 2011.igem.org

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='''Protocol'''=
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Listed below our protocol for this project.<br>
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We hope you find them useful!!
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= '''Protocol''' =
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<html><a name="cloning"></a></html>
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=== Medium for drosophila ===
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[[File:Cloning.PNG|link=https://2011.igem.org/Team:Kyoto/Cloning|Cloning]]<br>
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::{| class="wikitable"
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[[File:Medium.PNG|link=https://2011.igem.org/Team:Kyoto/Medium|Medium]]<br>
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|+ [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Medium for drosophila]
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[[File:Measurement.PNG|link=https://2011.igem.org/Team:Kyoto/Measurement|Measurement]]
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! width="300" |Materials
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! width="300" |Methods
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* water : 500mL
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* dry yeast : 20g
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* corn flour : 45g
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* glucose : 50g
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* agarose : 3.5~5g
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* propionic acid : 1.5mL
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* 10% p-hydroxybenzoate in 70% Eternol : 5g
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# Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
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# Stir corn flour and glucose with the remaining water.
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# Stir 1 and 2, then autoclave it again.
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# after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■
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Latest revision as of 02:26, 6 October 2011

Protocol

Listed below our protocol for this project.
We hope you find them useful!!

Cloning
Medium
Measurement

Retrieved from "http://2011.igem.org/Team:Kyoto/Protocol"