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Team:XMU-China/Project/Description
From 2011.igem.org
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We have developed a series of devices which program a bacteria population to maintain at different cell densities. We have designed and characterized the genetic circuit to establish a bacterial ‘population-control’ device in ''E. coli'' based on the well-known quorum-sensing system from ''Vibrio fischeri'', which autonomously regulates the density of an ''E. coli'' population. The cell density however is influenced by the expression levels of a killer gene (''ccdB'') in our device. As such, we have successfully controlled the expression levels of ''ccdB'' by site-directed mutagenesis of a ''luxR'' promoter (''lux pr'') and error-prone PCR of gene ''luxR'', and finally we have built a database for a series of mutation sites corresponding to different cell densities. An artificial neural network has then been built to model and predict the cell density of an ''E. coli'' population. This work can serve as a foundation for future advances involving fermentation industry and information processing. | We have developed a series of devices which program a bacteria population to maintain at different cell densities. We have designed and characterized the genetic circuit to establish a bacterial ‘population-control’ device in ''E. coli'' based on the well-known quorum-sensing system from ''Vibrio fischeri'', which autonomously regulates the density of an ''E. coli'' population. The cell density however is influenced by the expression levels of a killer gene (''ccdB'') in our device. As such, we have successfully controlled the expression levels of ''ccdB'' by site-directed mutagenesis of a ''luxR'' promoter (''lux pr'') and error-prone PCR of gene ''luxR'', and finally we have built a database for a series of mutation sites corresponding to different cell densities. An artificial neural network has then been built to model and predict the cell density of an ''E. coli'' population. This work can serve as a foundation for future advances involving fermentation industry and information processing. | ||
| - | + | [[Image:XMU_China_133.jpg|left|Figure 1 The bacteria population-control device|frame|Figure 1 The bacteria population-control device.]] | |
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| - | Figure 1 The bacteria population-control device. | + | |
This circuit incorporates a mechanism for programmed death in respond to changes in the environment, and allows us to probe the design principles of its more complex natural counterparts. | This circuit incorporates a mechanism for programmed death in respond to changes in the environment, and allows us to probe the design principles of its more complex natural counterparts. | ||
Revision as of 01:05, 6 October 2011
Project Description
i-ccdB: intelligent Control of Cell Density in Bacteria
We have developed a series of devices which program a bacteria population to maintain at different cell densities. We have designed and characterized the genetic circuit to establish a bacterial ‘population-control’ device in E. coli based on the well-known quorum-sensing system from Vibrio fischeri, which autonomously regulates the density of an E. coli population. The cell density however is influenced by the expression levels of a killer gene (ccdB) in our device. As such, we have successfully controlled the expression levels of ccdB by site-directed mutagenesis of a luxR promoter (lux pr) and error-prone PCR of gene luxR, and finally we have built a database for a series of mutation sites corresponding to different cell densities. An artificial neural network has then been built to model and predict the cell density of an E. coli population. This work can serve as a foundation for future advances involving fermentation industry and information processing.
This circuit incorporates a mechanism for programmed death in respond to changes in the environment, and allows us to probe the design principles of its more complex natural counterparts.
