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Team:SJTU-BioX-Shanghai/Project/Subproject1/Results
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[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_2|'''tRNA Modulator + Reporter for Quantitative Analysis ''']] | [[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_2|'''tRNA Modulator + Reporter for Quantitative Analysis ''']] | ||
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'''Action:''' when rare tRNA<sup>Arg</sup> is over expressed, the amount of tRNA<sup>Arg</sup> in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the modified luciferase's mRNA. | '''Action:''' when rare tRNA<sup>Arg</sup> is over expressed, the amount of tRNA<sup>Arg</sup> in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the modified luciferase's mRNA. | ||
*'''Results Conclusion''' | *'''Results Conclusion''' | ||
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===aaRS Modulator System=== | ===aaRS Modulator System=== | ||
Revision as of 20:46, 5 October 2011
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ResultsAction: Combinations of Modulators and ReportersSelect a Modulator and a Reporter, then click
tRNA Modulator System
tRNA Modulator + Reporter for Qualitative Analysis Action: when rare tRNAArg is over expressed, the amount of tRNAArg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA. tRNA Modulator + Reporter for Quantitative Analysis Action: when rare tRNAArg is over expressed, the amount of tRNAArg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the modified luciferase's mRNA.
aaRS Modulator System
aaRS Modulator + Reporter for Qualitative Analysis Action: When TDRS is expressed under IPTG induction, it can charge tRNAAsp-AGG with Asp. tRNAAsp-AGG then recognizes the rare codon AGG on RFP's mRNA and add Asp to the polypeptide chain. RFP is expressed. aaRS Modulator + Reporter for Quantitative analysis Action: When TDRS is expressed under IPTG induction, it can charge tRNAAsp-AGG with Asp. tRNAAsp-AGG then recognizes the rare codon AGG on the modified luciferase's mRNA and add Asp to the polypeptide chain. Modified luciferase is expressed.
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